Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2012 Vol.22 Issue.5 Published 2012-09-25

Article

	369	Umbilical cord blood MSCs transplantation myocardial repair of acute myocardial infarction in rats
		GONG Min， LI Tao， LEI Jing-wei， XIN Wei-chuan
		Objective: To observe the induction of myocardial ischemia microenvironment in vivo, and how human umbilical cord blood mesenchymal stem cells(UCBMSCs) differentiate directly to myocardiallike cells, and their effect on the heart function and revascularization in acute myocardial infarction(AMI) rat and to explore their possible mechanism. Methods: Collected health maternal cord blood cells; density gradient centrifugation and differential speed adherent was used to isolate MSCs. Thirty healthy adult SD rats was preparated to establish rat AMI model. They were divided into 2 groups: transplantation group was peripheral injected of transplanted GFPlabeled UCBMSCs, and control group injected of normal saline. Four weeks later, echocardiography figure detection and hemodynamic check was used, and heart tissue frozen section was harvested to be tested by immunofluorescence staining cbMSCs myocardial specificity protein cTnT and Connexin43 expression; and antiⅧ immunohistochemical staining, myocardial capillary density(MCD)as well. Results: Compared with the control group, 4 weeks after transplantation UCBMSCs expression of cardiacspecific proteins cTnT Connexin43, the control group had no cardiacspecific protein expression; transplantation group LVEDD, LVESD significantly reduced LVEF, LVFS increased significantly,and the hemodynamic indicators improved significantly; immunohistochemical staining showed that the transplantation group infarct border zone MCD increased significantly compared with the control group, the transplantation group(4.16± 0.2)/HP(×400), and the control group(2.29±0.3)/HP; difference between the two groups was significant(P<0.01) Conclusion：UCBMSCs transplantation was survival after rats AMI in their infarct parts and cardiomyocytelike cell differentiated; UCBMSCs transplantation significantly improved AMI cardiac function and ventricular remodeling, and promoted angiogenesis.
		2012 Vol. 22 (5): 369-373 [Abstract] ( 1207 ) [HTML 1KB] [ PDF 3444KB] ( 1914 )

	374	Impact of natural salt powder inhalation treatment in asthmatic mice to the pathological changes of lung and blood serum levels of IL-4,IL-5 and IL-13
		FAN Li-Juan, Xu-Hua-Jun, WU Zhan-Ao, Duan-Li-Rong
		Objective: To investigate the pathological changes of lung and the influence to serum IL-4, IL-5 and IL-13 levels in asthmatic mice treated by salt powder inhalation. Methods: Forty Balb/c female mice were divided randomly into control group, model group, therapy group A, therapy group B with each group of 10 only. On day 0 and 7, the mice of model group, therapy group A and therapy group B were injected intraperitoneal with liquid sensitized(ovalbumin), while the mice of control group with saline. On day 14, the mice inhaled the aerosol of 1% ovalbumin to induce asthma for the first time. On day 15, therapy group A and therapy group B were treated with salt powder inhalation for 30 min and 60 min every day, respectively. The control group and the model group were treated with air inhalation. On day 22, 23, 24, the asthma symptoms were induced with the same method continuously. On day 25, all mice were put into death. The lungs of mice were observed by pathological method. The levels of serum IL-4, IL-5, IL-13 were tested by ELISA. Results: Compared with control group, the lungs of model group mice had bronchial constriction, mucosal epithelial necrosis, inflammatory cells infiltration with eosinophils gathered. Compared with model group, the therapy group A and therapy group B improved markedly. The levels of serum IL-4, IL-5 of model group were higher than those of the control group obviously(P＜0.01). And the level of IL-13 of model group was high too(P＜0.05). The levels of IL-4, IL-5 and IL-13 of therapy group A and therapy group B were lower than those of model group significantly(P＜0.01).Conclusion: Inhaling natural salt powder could alleviate airway inflammation of bronchial asthma mice, and reduce the levels of related cytokines IL-4, IL-5 and IL-13 in serum.
		2012 Vol. 22 (5): 374-377 [Abstract] ( 1920 ) [HTML 1KB] [ PDF 3154KB] ( 2161 )

	378	Effect of α-lipoic acid on expression of transforming growth factor β1 and connective tissue growth factor in diabetic nephropathy of rats

		Objective: Transforming growth factor(TGF-β1) in diabetic nephropathy renal tissue of rats by α-lipoic acid on expression of connective tissue growth factor(CTGF), to study the delay of the role and mechanisms of renal fibrosis, to provide experimental basis for the prevention and treatment of diabetic nephropathy. Methods: Fifty healthy SD rats were divided randomly into 5 groups: normal control group，model of diabetes group，α-lipoic acid 15,30,60 mg/(kg·d) dose group. STZ single injection of 60 mg/kg in the lower left abdominal rat model of diabetes. α-lipoic acid is low, medium and high dose groups, respectively, 15,30,60 mg/(kg·d) orally for 12 weeks. Detected in each group of blood glucose, kidney weight index, and had the preparation of kidney paraffin sections, to determinate glomerular volume(MGA) and calculate the average glomerular volume(MGV). Fasting blood glucose was detected before the end of the experiment with SureStep Plus; HE staining under the light microscope was performed to observe the changes in glomerular volume, glomerular basement membrane; renal tissue TGF-β1 and CTGF expression was detected by immunohistochemical method. Results: Compared with normal group, blood glucose in diabetic rats was significantly increased; blood glucose in α-lipoic acid treatment group was significantly reduced compared with the diabetic group; In diabetic model rats, kidney weight index was significantly increased, light microscopy showed that glomerular volume of rats in diabetic model group increased significantly, mesangial widening the capillary lumen pressure, glomerular basement membrane thickening, renal tissue TGF-β1 and CTGF protein expression was decreased; diabetic model group MGA and MGV expanded significantly, compared with the treatment group there were varying degrees of narrowing. Conclusion: α-lipoic acid to play its anti-fibrosis by inhibiting the renal tissue of TGF-β1 and CTGF expression, and delaying the development of diabetic rat kidney fibrosis.
		2012 Vol. 22 (5): 378-381，386 [Abstract] ( 2156 ) [HTML 1KB] [ PDF 3713KB] ( 2441 )

	382	Effect of gene CTGF transfection on the expression of MMP-2 and MMP-9 and proliferation in human cervical cancer cells
		XIAO Wei, JIAO Xia1 QIAN Hua，et al
		Objective: To investigate the effect of connective tissue growth factor (CTGF) on the proliferation in human cervical cancer cell line Hela, with a focus on the expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9), and explore the underlying mechanism for the role of CTGF in the development of cervical cancer. Methods: pcDNA3.0CTGF and pcDNA3.0 were transfected into Hela cells through lipofectamine and positive clones which were screened by G418. Fluorescence quantitive polymerase chain reaction(FQPCR) and Western blot were employed to identify mRNA and protein expression of CTGF in Hela cells, respectively. The expression of MMP-2 and MMP-9 in positive clones was detected by FQPCR and Western blot. Cell viability was assessed by dimethylthiazoldiphenyltetrazoliumbromide (MTT) method. Results: Positive clone C16 with CTGF overexpression were successfully established. Compared with nontransfected control group, the expression of MMP-2 and MMP-9 in C16 were increased significantly,and the proliferation level of C16 was increased significantly. Conclusion: CTGF transfection could effectively enhance the expression of MMP-2 and MMP-9 and the proliferation in Hela cells which suggested a potential role for CTGF in gene therapy of cervical cancer.
		2012 Vol. 22 (5): 382-386 [Abstract] ( 1606 ) [HTML 1KB] [ PDF 3462KB] ( 1736 )

	387	An improvement approach of identification of small RNAs in prokaryote

		Objective: To construct an improvement approach of identification small RNAs(sRNAs) in prokaryote. Methods: Taking Yersinia pestis as a model, constructed cDNA library by combining the RACE(rapid amplification of cDNA ends) technique with advanced RNA size selecting protocol and diminishment of rRNAs, and finally verified the results using Northern blot. Results: Compared with currently methods, the new constructed method was inclined to gain authentic sRNAs with full length and rich abundance. Conclusion: An improved method of cDNA library construction was prepared successfully, by which more fulllength prokaryotic sRNAs can be acquired.
		2012 Vol. 22 (5): 387-390 [Abstract] ( 1504 ) [HTML 1KB] [ PDF 2789KB] ( 2803 )

	391	Expression of TLR2，TLR4 on doxorubicininjured cardiac cells in newborn rats
		SHU Jin, LIU Xin, WANG Hai-bo
		Objective: To study the expression of Tolllike receptor 2（TLR2）,Tolllike receptor 4（TLR4） on doxorubicininjured cardiac cells in newborn rats，and to discuss the possible mechanism on doxorubicininjured cardiac cells. Methods: Doxorubicin was administered to establish the injury model of primary cultured cardiomyocytes in newborn rats. Primary cultured cardiomyocytes were randomly divided into two groups: control group：myocardial cell suspension continued cultivate with serumfree medium and no drugs put into; DOX group: with doxorubicin 1 mg/L in cultured fluid.The expression of TLR2 and TLR4 protein in cardiac cells was checked with immunohistochemical after they were operated for 6 hours. The semiquantitative analysis of TLR2 and TLR4 protein was checked with Western blot after they were operated for 2 hours, 6 hours respectively. Results: The expression of TLR2 and TLR4 on the cardiac cells in DOX group are markedly increased when compared with control group(P<0.01). Conclusion: TLR2 and TLR4 may mediate and take part in the innate immune response of doxorubicininjured cardiac cells as a pattern recognition,and this was one of the cardiac cells injuring mechanism.
		2012 Vol. 22 (5): 391-393 [Abstract] ( 1294 ) [HTML 1KB] [ PDF 2584KB] ( 1977 )

	394	To develop a loop mediated isothermal amplification assay for quick detection of Listeria monocytogenes
		YAO Dong, ZHANG Ru-sheng, OU Xin-hua,et al
		Objective: To establish a loopmediated isothermal amplification(LAMP) assay for the rapid and specific detection of Listeria monocytogenes. Methods: Four primers regions on the hlyA gene of Listeria monocytogenes were designed and used for LAMP assay. Listeria monocytogenes DNA was amplified under isothermal conditions(65℃)for 60 min in water bath, then the amplified product was judged by naked eye, SYBR Green l staining, and electrophoresis analysis. To evaluate the specificity of the assay, 1 strain of Listeria monocytogenes and 10 strains of noneListeria monocytogenes were tested by LAMP and conventional PCR assay. In addition, the detection limit of LAMP was compared with that of PCR by using the Listeria monocytogenes strain，that were serially diluted and were amplified by LAMP and PCR. Results: With one strain of Listeria monocytogenes, observation with naked eyes, SYBR Green I staining and electrophoretic analysis were able to detect the products in the LAMP assay，and amplification were not observed when 10 strains of nonListeria monocytogenes were tested. The sensitivity of LAMP was higher than that of PCR assay. The detection limit of LAMP assay for Listeria monocytogenes was 3 cfu/ml and that of PCR was>300 cfu/ml. LAMP method was superior to conventional PCR for its rapid detection of Listeria monocytogenes, which can complete in 60 min. Conclusion: LAMP for rapid and specific detection of Listeria monocytogenes was established in this study.
		2012 Vol. 22 (5): 394-397 [Abstract] ( 1055 ) [HTML 1KB] [ PDF 3641KB] ( 2273 )

	398	Change of serum gammaglutamyltransferase in newlydiagnosed type 2 diabetes treated with shortterm continuous subcutaneous insulin infusion
		DONG Si-Jing, Ye Jing-Jing, Zhang Cai-Li, et al
		Objective: To investigate the effects of shortterm continuous subcutaneous insulin infusion (CSII) on serum gammaglutamyltransferase (GGT) levels in patients with newlydiagnosed type 2 diabetes mellitus, and to study the association between insulin resistance and GGT. Methods: Thirtytwo patients with newlydiagnosed type 2 diabetes mellitus were treated with CSII for 2 weeks. Oral glucose tolerance test and insulin releasing test were performed before and after intensive insulin therapy, meanwhile, blood lipid, serum GGT and other items were measured. Results: ① Two weeks of CSII treatment in type 2 diabetic patients resulted in significant reduction in fasting serum insulin［(11.60±2.89） vs(6.82±1.01)mmol/L, P<0.01］, postprandial serum insulin［(22.52±4.57）vs(13.81±3.10)mmol/L, P<0.01］ and homeostasis model assessment for insulin resistance(HOMAIR)\[2.20(1.45~3.07) vs 1.37(0.89~1.90), P<0.01\]. ② After CSII treatment for 2 weeks, the level of serum GGT was decreased significantly \[24.00(18.00~35.00) vs 18.00(16.00~27.00) U/L, P=0.005\]. ③ Serum GGT level was positively correlated with HOMAIR（r=0.418，P<0.05）. Meanwhile, changes in serum GGT levels were positively correlated with changes in HOMAIR（r=0.433，P<0.05）. ④ The multiple regression analysis showed that the HOMAIR were independent related factors in the influence of the serum GGT levels．Conclusion: In type 2 diabetic patients, elevated serum GGT levels were significantly decreased after CSII treatment. It might indicate that serum GGT plays an important role in the development of insulin resistance．
		2012 Vol. 22 (5): 398-401 [Abstract] ( 1310 ) [HTML 1KB] [ PDF 2703KB] ( 1822 )

	402	Expression and clinical significance of ARHⅠ and DAPK in Human Glioma
		HE Rui-1, 2 , Yuan Zhi-Cheng-1, Zhan Li-Ping-1, Li Qiao-Yu-1
		Objective: To investigate the expressions of aplysia ras homolog Ⅰ(ARHⅠ) and death associated protein kinase(DAPK) in normal brain tissues and glioma tissues and to analyse their clinical pathological significance. Methods: The SP immunohistochemical method was used to detect the expressions of ARHⅠ ans DAPK in 10 case of normal brain tissues and 47 case of human glioma samples. Results: The positive of ARHⅠ and DAPK were the highest in normal brain tissues.In glioma samples of different grades, the expression levels of ARHⅠ and DAPK decreased significantly with the increase of tumor malignancy. There was significant difference in the positive expression of ARHⅠ and DAPK between high grade and low grade glioma tissues.The difference was significant(P<0.05). The expression of ARHⅠ was correlated positively with the expression level of DAPK(r=0.723,P<0.05). Conclusion: The expression of ARHⅠ and DAPK were closely correlated with the clinical pathological grades. The downregulation of ARHⅠ and DAPK was involved in the initiation and progression of glioma.
		2012 Vol. 22 (5): 402-405 [Abstract] ( 1192 ) [HTML 1KB] [ PDF 3579KB] ( 1820 )

	406	Effect of shortterm continuous subcutaneous insulin infusion on the level of lactate dehydrogenase in newly diagnosed type 2 diabetic mellitus
		ZHANG Cai-Li, Dong Si-Jing, Ye Jing-Jing, et al
		Objective: To observe the level of serum lactate dehydrogenase（LDH）and to observe the effect of shortterm continuous subcutaneous insulin infusion(CSII) on LDH in newly diagnosed T2DM, and to investigate the association between insulin resistance and LDH. Methods: Thirtysix cases as NGT group and 37 newly diagnosed T2DM patients were involved in this study．The newly diagnosed T2DM patients were treated with CSII for 2 week．Before and after CSII therapy, 75 g oral glucose tolerance test(OGTT) were performed.The level of plasma glucose and insulin were examined respectively, serum LDH and other items were also measured. Results: ① Serum LDH levels in the T2DM group（177.9±43.01)U/L were higher than the NGT group(161.7±19.41） U/L (t=2.130，P<0.05). ② In the T2DM group, there was a significant decrease in fasting plasma glucose (FPG)and 2 h plasma glucose after glucose overload (2hPG) (P <0.01), a significant decrease in homeostasis model assessment for insulin resistance (HOMAIR) values \[2.00(1.35～2.61）vs 1.30(0.91～1.80) \],△INS30/△G30 and △INS120/△G120 were increased markedly\[（1.11±0.95） vs(2.25±2.14)，(1.22±1.14) vs (3.57±3.06)\](P <0.01),serum LDH concentrations were significantly decreased (t=2.397，P<0.05) after CSII treatment for 2 weeks. ③ In the T2DM group, serum LDH levels positively correlated with HOMAIR(r=0.359，P<0.05) . ④ The multiple regression analysis showed that HOMAIR was an independent related factor in the influence of the LDH concentrations. Conclusion: In the T2DM group insulin resistance and islet βcell function were improved and serum LDH concentrations were significantly decreased after two weeks CSII treatment. The levels of LDH were positively associated with insulin resistance.
		2012 Vol. 22 (5): 406-409,413 [Abstract] ( 1416 ) [HTML 1KB] [ PDF 3366KB] ( 1886 )

	410	Expression and significance of nerve growth factor in glioma and cerebrospinal fluid
		LU Xin-Yu, Lu Pei-song, Li Qiao-yu, Yang Yong
		Objective: To study the expression and clinical significance of nerve growth factor(NGF) in glioma and cerebral spinal fluid(CSF). Methods: A total of 86 cases of glioma and CSF were determined for NGF expression by immunohistochemical method(Envision) and ELISA. Ten cases of tissue samples from patients with traumatic brain injury who had received decompression surgery and 10 cases of CSF samples from patients underwent spinal anesthesia without nervous system disease were taken as the corresponding control groups. Results: In glioma and CSF cases，the NGF expression levels was higher than that in corresponding two control groups, the difference was statistically significant(P＜0.05)．There was significant difference in NGF expression between different grades of glioma(P＜0.05)；the expression of NGF increased in parallel with the increase of pathological grade of glioma(P＜0.05); and there was also significant difference in glioma incidence between the supratentorial tumour and infratentorial tumour(P＜0.05). Conclusion: NGF expression was a consistent feature of cerebral tumor, especially in glioma, and its expression was correlated with the genesis and development of glioma.
		2012 Vol. 22 (5): 410-413 [Abstract] ( 1038 ) [HTML 1KB] [ PDF 2927KB] ( 1783 )

	414	Relationship between ALDH2, ADH1B variant alleles and risk of esophageal cancer lymphatic metastasis
		FANG Na, Zhong Xi-Ming, Fan Yu
		Objective: To investigate the relationship between ADH1B rs1229984 and ALDH2 rs671 polymorphisms and the susceptibility to lymphatic metastasis in esophageal cancer in a Chinese Han population. Methods: Genotypes were determined by matrixassisted laser desorption/ionizationtime of flight mass spectrometry(MALDITOF MS) method in 85 esophageal cancer lymphatic metastasis positive patients and 270 lymphatic metastasis negative patients. The associations between ALDH2 rs671 G>A and ADH1B rs1229984 A>G genotypes and the risk of esophageal cancer lymphatic metastasis were estimated by computing the odds ratio(OR) and their 95% confidence interval(CI). Results: The ALDH2 rs671 G>A genotype frequencies were 58.8%(GG), 38.8%(GA), 2.4%(AA) in the lymphatic positive group and 59.3%(GG), 37.4%(GA), 3.3%(AA) in the lymphatic negative group, respectively(χ² =0.237, P=0.888)； logistic regression analyses revealed that ALDH2 rs671 AA genotype could reduce the risk of lymphatic metastasis(adjusted OR=0.70, 95% CI=0.14-3.45) compared with ALDH2 rs671 GG genotype, but the difference was not statistically significant. The ADH1B rs1229984 A>G genotype frequencies were 38.8%(AA), 50.6%(AG), 10.6%(GG) in the lymphatic positive group and 42.8%(AA), 41.6%(AG), 15.6%(GG) in the lymphatic negative group, respectively(χ² =2.553, P=0.279); logistic regression analyses revealed that ADH1B rs1229984 GG genotype could reduce the risk of lymphatic metastasis(adjusted OR=0.66, 95% CI=0.29-1.53) compared with ADH1B rs1229984 AA genotype, but the difference was not statistically significant. Conclusion: ALDH2 rs671 G>A and ADH1B rs1229984 A>G polymorphisms might not be associated with the risk of esophageal cancer lymph node metastasis， and awaits further investigation.
		2012 Vol. 22 (5): 414-417 [Abstract] ( 2766 ) [HTML 1KB] [ PDF 2823KB] ( 2322 )

	418	Expressions of CD123 in childhood Blineage acute lymphoblastic leukemia and the application of CD123 in minimal residual disease detection
		JI Xue-Qiang, Ji-Zheng-Hua, Shao-Hui-Jiang, et al
		Objective: Detecting the expression of CD123 in childhood Blineage acute lymphoblastic leukemia (B-ALL) and discussing the application and significance of CD123 in minimal residual disease (MRD) detection. Methods： With the normal children bone marrow lymphocytes as control, multiparameter flow cytometry was used to study the bone marrow lymphocytes immunophenotype and the expression of CD123 in 91 cases of children with B-ALL, 78 cases of which were underwent bone marrow cells chromosome cultivation and cytogenetic analysis. 65 cases of B-ALL children with CD123 positive expression were screened and monitored by CD10/CD123/CD34/CD19 in MRD detection. Results： The expression of CD123 in normal children bone marrow lymphocytes was negative, while 65 cases of 91 in B-ALL children was positive(positive rate 71.43%), and the expression level of CD123 was negatively correlated with the leukemic cells maturity. When children had a high expression of CD34, the expression of CD123 was also in a high level. The results of cytogenetic analysis showed that hyperdiploid B-ALL children had a higher level of CD123 expression, when comparing with the nonhyperdiploid cases. Fortyseven cases of B-ALL children (51.65%) had a high level of CD123 expression, and CD123 could be used as an effective marker for MRD monitoring. Conclusion： Most of B-ALL children had an expression of CD123, which was negatively correlated with the leukemic cells maturity. CD123 can be used as a maker for MRD monitoring in childhood B-ALL.
		2012 Vol. 22 (5): 418-421 [Abstract] ( 2588 ) [HTML 1KB] [ PDF 2757KB] ( 2296 )

	422	Expression and clinical significance of miR-146a in human esophageal squamous cell carcinoma
		JIN Li-Yan-1, Chen De-Yu-2, Mao Chao-Ming-2
		Objective: To investigate the expression of miR-146a in esophageal squamous cell carcinoma(ESCC) and analyze its correlation with clinicopathological features of ESCC. Methods: Stemloop RTPCR was used to detect the expression of miR-146a in 50 ESCC tissues and matched tumorfree tissues, and to analyze its correlation with clinicopathological features of ESCC. Results: The expression of miR-146a was significantly increased in ESCC tissues compared with the matched tumorfree tissues(Z=-3.162，P<0.01). Upregulation of miR-146a expression was correlated with differentiation degree, lymph node metastasis and clinical stage in ESCC(P<0.01). Conclusion: MiR-146a in ESCC tissues was significantly higher than that in matched tumorfree tissues, which may play a significant role in the occurrence and development of ESCC.
		2012 Vol. 22 (5): 422-425 [Abstract] ( 1128 ) [HTML 1KB] [ PDF 3003KB] ( 1816 )

	426	Effect of atorvastatin, alone and in combination with ezetimibe in the treatment of patients with acute coronary syndrome
		WANG Qiang, Sun-Jian-Hui
		Objective: To evaluate the efficacy and security ofatorvastatin combination with ezetimibe in patients with acute coronary syndrome(ACS). Methods: A total of 79 patients with ACS were randomized to receive atorvastatin (20 mg/d) and ezetimibe (10 mg/d, combination group, n=41) or atorvastatin (20 mg/d) alone (atorvastatin group, n=38). Levels of lipids of two groups were detected as well as ALT and CK after two weeks therapy. The cardiovascular events and related sideeffects were observed during two weeks of the treatment. Results: Compared to baseline, the levels of total cholesterol, triglyceride and low density lipoproteincholesterol were reduced after two weeks in two groups. The levels of cholesterol in combination group were significantly reduced than those in atorvastatin group (P<0.05).The incidence of cardiovascular events (recurrence of angina pectoris and acute myocardial infarction) in combination group was lower than that in atorvastatin group (P<0.05). Conclusion: The combined atorvastatin and ezetimibe therapy early after ACS had good security, and was superior to atorvastatin alone on reducing the levels of cholesterol and the incidence of cardiovascular events.
		2012 Vol. 22 (5): 426-429 [Abstract] ( 1144 ) [HTML 1KB] [ PDF 2547KB] ( 1862 )

	430	Effect of continuous positive airway pressure treatment on serum angiotensin converting enzyme levels in patients with obesityassociated obstructive sleep apnea syndrome
		SHEN Wen-Fu, Li-Hong-Miao, Wang-Qiang, et al
		Objective: To investigate the influence of continuous positive airway pressure(CPAP）treatment on the serum angiotensin converting enzyme levels in patients with obstructive sleep apnea syndrome(OSAS).Methods： Based on apnea hypopnea index(AHI) measured by polysomnography, 43 patients were diagnosed as OSAS(OSAS group) and enrolled in this study. The serum ACE levels of OSAS group were measured and compared with the subjects without OSAS(normal groups). In patients with OSAS, the relationship between levels of ACE and AHI were analyzed. Results： Compared with normal group［(38.95±21.83） U/L］, serum ACE levels in OSAS group［(23.35±15.62） U/L，P<0.05］ were significantly reduced before CPAP treatment. In OSAS group, serum ACE level after CPAP treatment［(24.55±14.89） U/L］ was similar to those before CPAP treatment(P>0.05). No relationship was observed in OSAS group between the ACE levels and AHI levels. Conclusion： The serum ACE levels in OSAS group was significantly higher than in normal group. The CPAP treatment cannot change the ACE level in OSAS patients. The serum ACE levels were not correlated with the AHI levels in OSAS patients.
		2012 Vol. 22 (5): 430-433 [Abstract] ( 918 ) [HTML 1KB] [ PDF 2805KB] ( 1600 )

	434	Clinical significance of interleukin 6, Creactive protein, neutrophil cell surface marker CD64 in the diagnostic value of neonatal infections
		DAI Biao
		Objective: To explore clinical significance of the interleukin 6 (IL-6), Creactive protein (CRP), neutrophil cell surface marker CD64 in the diagnostic value of neonatal infections.Methods: The information of 107 cases were analyzed retrospectively; they were from Yixing people′s hospital, department of neonatology, admitted in January 2005 to December 2011. They were divided into 35 cases of sepsis group, 30 cases of nonbacterial infection, 42 cases of healthy newborns. Positive rate of IL-6 and CRP, and CD64 levels were analyzed.Results: CRPpositive rate of sepsis group、 nonbacterial infection group and the healthy controlgroup was 80%, 63.3%, 23.8% respectively.There were significant differences between the septicemia group and the healthy control group, or the nonbacterial infection group and the healthy control group（all P<0.05）. IL-6 positive rate of the three groups was 85.7%, 33.3%, 9.5% respectively , and the differences were statistically significant between the sepsis group and nonbacterial infection, or sepsis group and the healthy control group (all P<0.05). The CD64 level differences among the three groups was statistically significant (H= 77.818, P<0.01). Specificity of the combined detection IL-6+CRP+CD64 were 94.6%, and the differences were statistically significant compared to IL-6, CRP, CD64, IL-6+ CD64, CRP+CD64 (all P<0.05). Conclusion: Combined detection of IL-6, CRP and CD64 will increase the specificity of bacterial infection diagnosis, and it will provide an early diagnostic marker for clinical work.
		2012 Vol. 22 (5): 434-436 [Abstract] ( 1244 ) [HTML 1KB] [ PDF 2019KB] ( 2343 )

	437
		GU Zheng-Song1, Wang Cun-Zu2 , Yuan Zhi-Cheng3, Yang Wei-Xian2

		2012 Vol. 22 (5): 437-439，443 [Abstract] ( 1286 ) [HTML 1KB] [ PDF 2680KB] ( 1751 )

	440	Association between different impaired glucose regulation and the earlystage arteriosclerosis
		ZHAO Jing-1, 2 , Liang-Jun-2, Dou Lian-Jun-2, et al
		Objective: To explore the associations of glucose exposure and carotidtofemoral pulse wave velocity (c-f PWV) in prediabetes adults. Methods: Fasting plasma glucose, 2hour oral glucose tolerance test (2h OGTT), hemoglobin A1c(HbA1c) and c-f PWV were analysed in 5 098 non-diabetes subjects. Results: We found that the differences in c-f PWV between individuals with impaired fasting glucose (IFG), impaired glucose tolerance (IGT), high HbA1c and those without these abnormalities were 0.97 m/s, 1.08 m/s and 0.92 m/s (P<0.01). In addition, our data indicated that individuals of both high HbA1c and IFG or IGT had significantly higher levels of c-f PWV compared with those who only had high HbA1c (P=0.036 and 0.03, respectively); or those only had IFG (P=0.02); or only had IGT (P=0.04). Conclusion: c-f PWV was associated with prediabetes, independent of metabolic risk factors. We found that individuals of both high HbA1c and IFG or IGT had additive effects on cf PWV, and all these alterations may contribute to development and progression of atherosclerosis.
		2012 Vol. 22 (5): 440-443 [Abstract] ( 1253 ) [HTML 1KB] [ PDF 2720KB] ( 1710 )

	444
		SHI Li-Min-1, Wang-Yan-1, Cui-Hong-Lian-2, Xu-Wen-Jiong-1, Shi-Qiu-Lei-3, et al

		2012 Vol. 22 (5): 444-447，451 [Abstract] ( 1701 ) [HTML 1KB] [ PDF 3374KB] ( 2670 )

	448	Clinical observation of levosimendan on patients with acute decompensated heart failure
		YIN Quan-Zhong, Cui-Jun-You, Zheng-Ruo-Long, et al
		Objective: To explore the clinical efficacy and safety of levosimendan on patients with acute decompensated heart failure. Methods： One hundred patients met the inclusion criteria were randomly divided into one group with levosimendan andthe other group with milrinone. Clinical efficacy, heart function, BNP and the 24 hourly urine volume, systolic pressure, LVEF, LVEDD and adverse reaction were detected before and after treatment. Results： Both efficiency and total effective rate after treatment in levosimendan group were higher than those in milrinone group (χ2=4.006 and 8.274，P<0.05). Statistical significance of BNP, the 24 hourly urine volume and LVEF was noted in two groups after treatment(P＜0.01).Serum BNP levels decreased, the total 24 hourly urine volume increased and LVEF improved in levosimendan group compared with milrinone group(P<0.01).During the treatment period, occurrences of adverse events in levosimendan group were lower than those in control group (χ2=1.301，P<0.05). Conclusion： Levosimendan was well tolerated and superior to milrinone for patients with decompensated heart failure refractory to conventional medications, because it was able to improve heart function and the symptoms of heart failure by alterring haemodynamic variables.
		2012 Vol. 22 (5): 448-451 [Abstract] ( 1109 ) [HTML 1KB] [ PDF 2689KB] ( 2302 )

	452
		LI Sheng-Ping, Hu-Jian-Ping, Zhang-Feng

		2012 Vol. 22 (5): 452-456 [Abstract] ( 1473 ) [HTML 1KB] [ PDF 3989KB] ( 2244 )

	457


		2012 Vol. 22 (5): 457-458 [Abstract] ( 1182 ) [HTML 1KB] [ PDF 1344KB] ( 1888 )

	459
		GENG Li-Hong-1, Meng-Fan-Wen-2

		2012 Vol. 22 (5): 459-460 [Abstract] ( 1045 ) [HTML 1KB] [ PDF 1369KB] ( 2201 )

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