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| An improvement approach of identification of small RNAs in prokaryote |
| JI Xiao-lan1， QU Yi2， HAN Yan-ping2，et al |
| 1.Department of Biochemistry, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013；2.State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China |
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Abstract Objective: To construct an improvement approach of identification small RNAs(sRNAs) in prokaryote. Methods: Taking Yersinia pestis as a model, constructed cDNA library by combining the RACE(rapid amplification of cDNA ends) technique with advanced RNA size selecting protocol and diminishment of rRNAs, and finally verified the results using Northern blot. Results: Compared with currently methods, the new constructed method was inclined to gain authentic sRNAs with full length and rich abundance. Conclusion: An improved method of cDNA library construction was prepared successfully, by which more fulllength prokaryotic sRNAs can be acquired.
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2012, Vol. 22 
