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| Impact of natural salt powder inhalation treatment in asthmatic mice to the pathological changes of lung and blood serum levels of IL-4,IL-5 and IL-13 |
| FAN Li-juan1，XU Hua-jun2, WU Zhan-ao3， DUAN Li-rong4 |
| (1.Department of Respiratory Medicine, the No.359 Hospital of Chinese PLA, Zhenjiang Jiangsu 212001； 2.Zhenjiang Salt Chemical Company,Zhenjiang Jiangsu 212001； 3.Department of Stomatology, the No.359 Hospital of Chinese PLA, Zhenjiang Jiangsu 212001； 4.School of Medical Science and Laboratory Medicine,Jiangsu University, Zhenjiang Jiangsu 212013，China) |
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Abstract Objective: To investigate the pathological changes of lung and the influence to serum IL-4, IL-5 and IL-13 levels in asthmatic mice treated by salt powder inhalation. Methods: Forty Balb/c female mice were divided randomly into control group, model group, therapy group A, therapy group B with each group of 10 only. On day 0 and 7, the mice of model group, therapy group A and therapy group B were injected intraperitoneal with liquid sensitized(ovalbumin), while the mice of control group with saline. On day 14, the mice inhaled the aerosol of 1% ovalbumin to induce asthma for the first time. On day 15, therapy group A and therapy group B were treated with salt powder inhalation for 30 min and 60 min every day, respectively. The control group and the model group were treated with air inhalation. On day 22, 23, 24, the asthma symptoms were induced with the same method continuously. On day 25, all mice were put into death. The lungs of mice were observed by pathological method. The levels of serum IL-4, IL-5, IL-13 were tested by ELISA. Results: Compared with control group, the lungs of model group mice had bronchial constriction, mucosal epithelial necrosis, inflammatory cells infiltration with eosinophils gathered. Compared with model group, the therapy group A and therapy group B improved markedly. The levels of serum IL-4, IL-5 of model group were higher than those of the control group obviously(P＜0.01). And the level of IL-13 of model group was high too(P＜0.05). The levels of IL-4, IL-5 and IL-13 of therapy group A and therapy group B were lower than those of model group significantly(P＜0.01).Conclusion: Inhaling natural salt powder could alleviate airway inflammation of bronchial asthma mice, and reduce the levels of related cytokines IL-4, IL-5 and IL-13 in serum.
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Received: 11 August 2012
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WANG Yü-cong1, WANG Jia1, LI Guo-hai2, FENG Chi1, JIANG Yang1, CHEN Jia-xin1, WANG Wen-xin1,PENG Hao1, HOU Zhuo-ran1, XU Jia-dan1, XIAO Xü1, WANG Lei1, ZHANG Wei-ning1. Effects of poly I:C induced prenatal immune challenge and maternal deprivation on schizophreniarelated behavior in adult mice offspring[J]. Journal of Jiangsu University(Medicine Edition), 2017, 27(5): 379-384,390. |
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2012, Vol. 22 
