|
|
|
| Effect of gene CTGF transfection on the expression of MMP-2 and MMP-9 and proliferation in human cervical cancer cells |
| XIAO Wei, JIAO Xia1 QIAN Hua，et al |
| 1.Department of Pathology, 2.Department of Gynaecology, 3.Department of Oncology, Taizhou People’s Hospital, Taizhou Jiangsu 225300, China |
|
|
|
|
Abstract Objective: To investigate the effect of connective tissue growth factor (CTGF) on the proliferation in human cervical cancer cell line Hela, with a focus on the expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9), and explore the underlying mechanism for the role of CTGF in the development of cervical cancer. Methods: pcDNA3.0CTGF and pcDNA3.0 were transfected into Hela cells through lipofectamine and positive clones which were screened by G418. Fluorescence quantitive polymerase chain reaction(FQPCR) and Western blot were employed to identify mRNA and protein expression of CTGF in Hela cells, respectively. The expression of MMP-2 and MMP-9 in positive clones was detected by FQPCR and Western blot. Cell viability was assessed by dimethylthiazoldiphenyltetrazoliumbromide (MTT) method. Results: Positive clone C16 with CTGF overexpression were successfully established. Compared with nontransfected control group, the expression of MMP-2 and MMP-9 in C16 were increased significantly,and the proliferation level of C16 was increased significantly. Conclusion: CTGF transfection could effectively enhance the expression of MMP-2 and MMP-9 and the proliferation in Hela cells which suggested a potential role for CTGF in gene therapy of cervical cancer.
|
|
Received: 15 June 2012
|
|
|
|
|
|
|
2012, Vol. 22 
