（1. School of Medicine， Jiangsu University， Zhenjiang Jiangsu 212013； 2. Department of Hematology， the Affiliated Peoples Hospital of Jiangsu University， Zhenjiang Jiangsu 212001，China）
Abstract：Objective: To investigate the expression and clinical significance of unconventional prefoldin RPB5 interactor(URI) in gastric cancer tissues as well as the effect of URI on the apoptosis of gastric cancer cells treated with cisplatin. Methods: Immunohistochemistry was used to detect the expression of URI in tissue microarray of gastric cancer. MGC803 and SGC7901 gastric cancer cells were cultivated in vitro. Hiperfect transfection reagents were used to transfect URI siRNA interference fragment into the two strains of gastric cancer cells respectively. The expression of URI in gastric cancer cells was detected by qRTPCR and Western blotting. Gastric cancer cells was divided into blank group, URI siRNA group and cisplatin induced untransfected group, scrambled group, URI siRNA group. Flow cytometry was used to detect the apoptosis of gastric cancer cells treated with cisplatin. Results: The expression of URI in cytoplasm of gastric cancer tissue was significantly higher than that in the adjacent tissues (P<0.05). The URI expression of cell nucleus in gastric cancer tissue was higher than that in paracancerous tissue, but the difference was not statistically significant. The expression of URI was not significantly correlated with the survival period and clinical stage of gastric cancer patients. The apoptosis rates of gastric cancer cells with URI knockdown were increased significantly compared to scrambled groups(P<0.01). Conclusion: As an oncoprotein, URI helped inhibitting apoptosis of gastric cancer cells and may play a role in gastric carcinogenesis.
卞慧琴1， 谷雨1，2， 陈瑾楠1， 王倩1， 吕耀娟1， 郑其平1， 谷俊侠1. URI在胃癌组织的表达及对顺铂诱导的胃癌细胞凋亡的影响[J]. 江苏大学学报:医学版, 2018, 28(05): 369-375.
BIAN Huiqin1， GU Yu1，2， CHEN Jin-nan1， WANG Qian1， LV Yao-juan1， ZHENG Qi-ping1， GU Jun-xia1. Expression of URI in gastric cancer tissues and its effect on
cisplatininduced apoptosis of gastric cancer cells. Journal of Jiangsu University(Medicine Edition), 2018, 28(05): 369-375.
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2018, Vol. 28 
