The role of piR-hsa-1282 in lipopolysaccharide-induced 293T cells injury
LI Yang1,2， WANG Ying1,2， WANG Yan1,2， SHI Xin-yu1,2， CHEN Jin1,2， QIAN Hui1,2
(1. Zhenjiang Key Laboratory of High Techonology Research on Exosomes Foundation and Transformation Application; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To investigate the role of piRhsa1282 in lipopolysaccharide(LPS)induced 293T cells injury. Methods: A 24 h LPStreated 293T cells was established as the acute injury model. qRTPCR was used for determing the expression level of piRhsa1282.ELISA was applied for detection of IL6 expression.Western Bloting was used to detect the expression of cleavedcaspase 3,cleavedcaspase 9 and IL1β after piRhsa1282 inhibition. Results: With stimulation of LPS for 24 h, cell viability was reduced and the expression levels of cleavedcaspase 3 and IL6 were upregulated.Meanwhile,there was a higher level of piRhsa1282. Compared with the control group,piRhsa1282 inhibition led to the downregulation of cleavedcaspase 3,cleavedcaspase 9 and IL1β. Conclusion: piRhsa1282 could be involved in LPSinduced injury through regulation of apoptosis and inflammatory response.
收稿日期: 2019-03-19
基金资助:
国家自然科学基金资助项目（81871496）；江苏大学学生科研项目（17A471）
通讯作者:
钱晖（通讯作者），教授，博士生导师，E-mail:lstmmmLst@163.com
作者简介: 李杨（1992—），女，硕士研究生
引用本文:
李杨1,2， 王颖1,2， 王妍1,2， 施鑫钰1,2， 陈瑾1,2， 钱晖1,2. piR-hsa-1282在脂多糖诱导的293T细胞急性损伤中的作用[J]. 江苏大学学报:医学版, 2019, 29(05): 386-389,393.
LI Yang1,2， WANG Ying1,2， WANG Yan1,2， SHI Xin-yu1,2， CHEN Jin1,2， QIAN Hui1,2. The role of piR-hsa-1282 in lipopolysaccharide-induced 293T cells injury . Journal of Jiangsu University(Medicine Edition), 2019, 29(05): 386-389,393.
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2019, Vol. 29 
