Abstract：Objective: To investigate the effects of malic enzyme 3(ME3) on the ability of mitochondrial fusion and fission in human glioma cells. Methods: The sh-ME3 plasmids and sh-EGFP(control plasmids ) were transfected into the glioma cell LN229. The plasmid knockdown efficiency were detected by real time PCR and Western blotting. Western blotting was used to detect the markers of apoptosis and mitochondrial fusion and fission, and membrane potential of mitochondria was detected by fluorescent probe. Results: Compared with the control group, the mRNA expression level of ME3 in the experimental group decreased by 76%(t=20.16 ,P<0.01),and the protein expression level also decreased; the mitochondrial fission related markers Fis-1 and DRP1 were increased, while the mitochondrial fusion related markers Mfn1, Mfn2, OPA1 were decreased, membrane potential of mitochondria was decreased; the apoptosis related marker cleaved-caspase 3 was increased,while Bcl-2 was decreased. Conclusion: The knockdown of ME3 promotes mitochondrial fission in glioma cells, inhibits their fusion, reduces membrane potential of mitochondria, thereby causes apoptosis.
［Key words］malic enzyme 3; glioma; mitochondrial; apoptosis
收稿日期: 2019-03-13
基金资助:
国家自然科学基金资助项目（81772694）
通讯作者:
龚爱华（通讯作者），教授，硕士生导师，E-mail:ahg5@163.com
作者简介: 程洁（1994—）,女，硕士研究生；
引用本文:
程洁1, 陈曦2, 唐宇1, 刘菲1, 龚爱华1. 干扰苹果酸酶3对人脑胶质瘤细胞线粒体分裂与融合的影响[J]. 江苏大学学报:医学版, 2019, 29(03): 185-188.
CHENG Jie-1, Chen-Xi-2, Tang-Yu-1, Liu-Fei-1, Gong-Ai-Hua-1. The roles of malic enzyme 3 knockdown in mitochondrial fusion and fission in human glioma cells. Journal of Jiangsu University(Medicine Edition), 2019, 29(03): 185-188.
2019, Vol. 29 
