Abstract：Objective: To investigate the effects of knockdown of Caveolin1 on the cell proliferation, apoptosis and reactive oxygen species (ROS) production in thyroid follicular cells Nthyori 31. Methods: Nthyori 31 cells were divided into the experimental group and negative control group, Caveolin1 knockdown virus supernatant and negative control virus supernatant were transfected, respectively, and the untransfected Nthyori 31 cells were used as the blank group. After 48 h infection, adding puromycin into the media to select positively infected cells, the expression of Caveolin1 was determined by qRTPCR and Western blotting. The cell proliferation was detected by MTT, the apoptosis rate was detected by flow cytometry, and the ROS level was detected by fluorescent probe DCFHDA. Results: The qRTPCR and Western blotting results showed that the lentiviral shRNA had a specific knockdown effect on the Cav1 mRNA and protein levels in Nthyori 31 cells (both P<0.01). Compared with the control group, Caveolin1 knockdown enhanced apoptosis, ROS production, and inhibited the proliferation of thyroid follicular cells (both P<0.01). Conclusion: Decreased Caveolin1 expression enhanced apoptosis and ROS production, inhibited the proliferation of thyroid follicular cells, which could play a role in the damage of thyroid follicular cells.
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2019, Vol. 29 
