Effects of ginsenoside-Rg1 on ischemia-induced angiogenesis in the mouse hindlimb
WANG Xiao-li1, JIN Yue1, LIU Lu1, ZHANG Kai1,YANG Nai-quan2
(1. Clinical Laboratory, 2. Department of Cardiology, Huai′an Second People′s Hospital Affiliated to Xuzhou Medical University, Huai′an Jiangsu 223002, China)
Abstract：Objective: To investigate whether Rg1 could improve angiogenesis in ischemic hindlimb of mice in vivo. Methods: Mouse hindlimb ischemia model was established by ligating the right femoral artery. Animals were randomly divided into the following two groups(n=10) for intraperitoneal treatment: Rg1(10 mg·kg-1·d-1) treated group and saline as control group. The blood flow was assessed by laser Doppler imaging at 2 weeks after surgery. Ischemic muscle was harvested for immunofluorescence analysis for CD31, TUNEL assay for apoptotic activity and colorimetric determination of nitric oxide(NO)production at 2 weeks postsurgery. Results: Rg1 promotes angiogenesis and blood reperfusion recovery. Moreover, Rg1 treatment could also significantly improve secretion of NO, and inhibit cell apoptosis in ischemic tissues. Conclusion: Rg1 promoted angiogenesis in ischemic hindlimb of mice and could be considered as a potential agent for therapeutic angiogenesis.
收稿日期: 2017-12-13
通讯作者:
杨乃全(通讯作者)，博士，E-mail: qnyang2005@163.com
作者简介: 王晓丽(1981—)，女，主管检验师；
引用本文:
王晓丽1， 金跃1， 刘鹭1， 张凯1， 杨乃全2. 人参皂苷Rg1改善小鼠肢体缺血后血管新生[J]. 江苏大学学报:医学版, 2018, 28(02): 126-128,134.
WANG Xiao-li1, JIN Yue1, LIU Lu1, ZHANG Kai1,YANG Nai-quan2. Effects of ginsenoside-Rg1 on ischemia-induced angiogenesis in the mouse hindlimb
. Journal of Jiangsu University(Medicine Edition), 2018, 28(02): 126-128,134.
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2018, Vol. 28 
