Abstract：Objective: To investigate the expression of lncRNA PIK3CDAS1 in renal cancer cells and its effect on the proliferation and apoptosis of renal cancer cells,and the genes related to the function of PIK3CDAS1 were explored. Methods: The expression of PIK3CDAS1 in normal kidney cells(HK2) and renal cancer cells (769P, 786O, A498) was detected by realtime fluorescence quantitative PCR(qRTPCR). The PIK3CDAS1 overexpression vector was transfected into renal cancer cells(769p) to construct a PIK3CDAS1 stable overexpression cell line. The effect of PIK3CDAS1 on the proliferation and apoptosis of renal cancer cells was detected by CCK8 assay and flow cytometry. The apoptosisrelated gene expression profile chip was used to screen genes that may be related to PIK3CDAS1 function. Results: The expression of PIK3CDAS1 was significantly decreased in kidney cancer cells (769P, 786O, A498) compared with normal renal cell HK2 (P<0.01 or P<0.05). The PIK3CDAS1 overexpression cell line was successfully constructed and verified by qRTPCR. The expression of PIK3CDAS1 in the experimental group was significantly higher than that of the negative control (P<0.05). The proliferation and apoptosis experiments showed that the proliferation ability of the cells in the experimental group was significantly decreased (P<0.01), and the apoptosis was significantly increased (P<0.01). The results of gene expression microarray screening showed that the genes interacting with PIK3CDAS1 and promoting apoptosis are DFFA, BCL2L1, and CASP9. Conclusion: PIK3CDAS1 may be a tumor suppressor gene and is lowly expressed in renal cancer cells. PIK3CDAS1 can inhibit the proliferation of renal cancer cells and promote apoptosis, and its proapoptotic function may be related to CASP9, DFFA, BCL2L1 genes.
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2018, Vol. 28 
