白细胞介素17体外抑制小鼠髓源抑制性细胞的凋亡

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摘要目的: 体外观察白细胞介素17（interleukin17，IL-17）对小鼠髓源抑制性细胞（myeloidderived suppressor cells, MDSCs）凋亡的影响。方法: 通过皮下注射Lewis细胞构建荷瘤小鼠模型，采用免疫磁珠分选技术（MACS）分离脾脏MDSCs，常规培养。经IL-17 处理24 h后，采用流式细胞术检测MDSCs凋亡率；经IL17处理6 h后通过蛋白质印迹法检测细胞Bcl-2的表达。结果： IL-17处理组活细胞数量为（9.547±0.962 6）×104，明显高于对照组活细胞数［（4.123±0.259 8）×104，P<0.01］；IL-17处理组活细胞比例为（59.55±2.831）%，明显高于对照组［（45.33±1.556）%，P<0.05］；对照组晚期凋亡细胞比例为（22.87±1.349）%，明显高于IL-17处理组［（13.70±1.003）%，P<0.01］。蛋白质印迹检测结果显示，IL-17处理后的MDSCs抗凋亡蛋白Bcl2表达水平明显上调（P<0.05）。结论： IL-17体外可明显抑制MDSCs的凋亡。

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关键词 ：白细胞介素17, 髓源抑制性细胞, 凋亡, Bcl-2

Abstract：Objective: To observe the apoptosis of myeloidderived suppressor cells (MDSCs) that is treated by IL17 and explore the effect of IL17 on apoptosis of MDSCs. Methods: Lewis lung carcinoma (LLC) tumor bearing models were constructed with subcutaneous injection of Lewis cells. MDSCs were isolated from spleen in tumorbearing mice and cultured in RPMI 1640 culture media. After treating MDSCs with IL17 for 24 h, apoptosis of MDSCs were detected by Annexin V and PI. Furthermore,Western blotting was also used to detect the expression of antiapoptotic protein Bcl2 after treating MDSCs with IL17 for 6 h. Results: The counts of viable cells of MDSCs were（4.123±0.259 8）×104 in control group and （9.547±0.962 6）×104 in IL17 treatment group (P<0.01); percentage of viable cells of MDSCs was (45.33±1.556)% in control group and (59.55±2.831)% in IL17 treatment group (P<0.05). The later apoptosis of MDSCs were (22.87±1.349)% in control group and (13.70±1.003)% in IL17 treatment group(P<0.01). Results of Western blotting showed that the expression level of antiapoptotic protein Bcl2 significantly increased after IL17 treatment(P<0.05). Conclusion: IL17 may inhibit the apoptosis of MDSCs in vitro significantly.

收稿日期: 2016-06-07

基金资助:

国家自然科学基金资助项目（(31470881,31270947)

通讯作者: 王胜军（通讯作者），教授，博士生导师，E-mail:sjwjs@ujs.edu.cn

作者简介: 王娟（1991—），女，硕士研究生；

引用本文:

王娟，田洁，田新宇，王运刚，许化溪，王胜军. 白细胞介素17体外抑制小鼠髓源抑制性细胞的凋亡[J]. 江苏大学学报:医学版, 2017, 27(01): 24-26. WANG Juan, TIAN Jie, TIAN Xin-yu, WANG Yun-gang, XU Hua-xi, WANG Sheng-jun. Suppressive effect of IL-17 on apoptosis of myeloid-derived suppressor cells. Journal of Jiangsu University(Medicine Edition), 2017, 27(01): 24-26.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2017/V27/I01/24

[1\]Chiba T, Marusawa H, Ushijima T. Inflammationassociated cancer development in digestive organs: mechanisms and roles for genetic and epigenetic modulation\[J\].Gastroenterology,2012,143(3): 550-563.
\[2\]Ma Y,Han W,Yang L,et al. The regulation of miRNAs in inflammationrelated carcinogenesis\[J\]. Curr Pharm Des, 2015, 21(21):3023-3031.
\[3\]Melani C,Chiodoni C,Forni G,et al. Myeloid cell expansion elicited by the progression of spontaneous mammary carcinomas in cerbB2 transgenic BALB/c mice suppresses immune reactivity\[J\]. Blood,2003,102(6):2138-2145.
\[4\]Voron T, Marcheteau E, Pernot S, et al. Control of the immune response by proangiogenic factors\[J\]. Front Oncol,2014, 4:70.
\[5\]Kitamura T, Fujishita T, Loetscher P, et al. Inactivation of chemokine (CC motif) receptor 1(CCR1) suppresses colon cancer liver metastasis by blocking accumulation of immature myeloid cells in a mouse model\[J\]. Proc Natl Acad Sci U S A,2010,107(29):13063-13068.
\[6\]Condamine T, Ramachandran I, Youn JI, et al. Regulation of tumor metastasis by myeloidderived suppressor cells\[J\]. Annu Rev Med,2015,66:97-110.
\[7\]Korn T, Bettelli E, Oukka M, et al. IL17 and Th17 cells\[J\]. Annu Rev Immunol,2009,27: 485-517.
\[8\]Bettelli E,Oukka M, Kuchroo VK. TH17 cells in the circle of immunity and autoimmunity\[J\]. Nat Immunol, 2007, 8(4): 345-350.
\[9\]Kenna TJ,Davidson SI,Duan R,et al.Enrichment of circulating interleukin17secreting interleukin23 receptorpositive γ/δ T cells in patients with active ankylosing spondylitis\[J\].Arthritis Rheum,2012, 64(5):1420-1429.
\[10\]Ruutu M, Thomas G, Steck R,et al. βglucan triggers spondylarthritis and Crohn′s diseaselike ileitis in SKG mice\[J\].Arthritis Rheum, 2012,64(7):2211-2222.
\[11\]Ma S, Cheng Q, Cai Y, et al. IL17A produced by γδ T cells promotes tumor growth in hepatocellular carcinoma\[J\]. Cancer Res,2014，74(7):1969-1982.
\[12\]Chen X, Xie Q, Cheng X, et al. Role of interleukin17 in lymphangiogenesis in nonsmallcell lung cancer: enhanced production of vascular endothelial growth factor C in nonsmallcell lung carcinoma cells\[J\]. Cancer Sci,2010,101(11):2384-2390.