黄芩苷对小鼠巨噬细胞干扰素基因刺激因子通路活化的影响

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摘要 目的: 研究黄芩苷对小鼠骨髓源巨噬细胞（bone marrowderived macrophages，BMDMs）中干扰素基因刺激因子（stimulator of interferon genes,STING）通路的效应。方法: 将C57BL/6小鼠骨髓细胞经巨噬细胞集落刺激因子诱导，培养制备BMDMs，将细胞分为6组：对照组（二甲基亚砜）、黄芩苷组、环鸟苷酸-腺苷酸（cGAMP）组、黄芩苷+cGAMP组、脂多糖组、黄芩苷+脂多糖组。用显微镜观察各组BMDMs的细胞形态；流式细胞术检测各组BMDMs表面CD80、CD86的表达；Griess法检测BMDMs培养上清液中一氧化氮的含量；ELISA法检测BMDMs IL-6、TNF-α和IFN-β的分泌。结果： 黄芩苷对BMDMs 的形态和生长状态无明显影响；黄芩苷抑制活化BMDMs的一氧化氮产生，负向调控BMDMs表面CD80、CD86的表达水平，抑制STING通路活化后巨噬细胞IL-6、TNF-α和IFN-β的分泌。结论： 黄芩苷抑制BMDMs中cGAMP激活的STING通路活化。

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关键词 ：黄芩苷, 巨噬细胞, 干扰素基因刺激蛋白, 环鸟苷酸-腺苷酸, 干扰素-β

Abstract：Objective: To determine the effects of Baicalin on stimulator of interferon genes （STING）pathway in murine bone marrowderived macrophages（BMDMs）. Methods: The murine bone marrow cells were used to induce BMDMs by macrophage colony stimulating factor（M-CSF）in vitro. Then, differentiated BMDMs were divided into six groups in the experiment: dimethyl sulfoxide（DMSO）control group, Baicalin group, cGAMP group, Baicalin+cGAMP group, LPS group and Baicalin+LPS group. The morphological characteristics of the BMDMs were observed under the microscope. The expression of CD80 and CD86 on BMDMs were detected by flow cytometry. The production of nitric oxide（NO）in the supernatant of BMDMs was determined through Griess method. The levels of IL6, TNFα and IFN-β in supernatant of BMDMs were analyzed using ELISA. Results: Baicalin treatment had no significant effects on the morphology and growth status of BMDMs, but it significantly inhibited nitric oxide secretion and the expression of CD80 and CD86 in BMDMs. Moreover, it also inhibited the secretion of IL6, TNFα and IFNβ in activated macrophages, through depressing the STING pathway in BMDMs. Conclusion: Baicalin restrains the activation of STING pathway which induced by cGAMP in BMDMs.

收稿日期: 2020-01-09

基金资助:国家自然科学基金资助项目（81871234，31570879）；江苏省社会发展重点项目（BE2017696）

通讯作者: 夏圣（通讯作者），教授，博士生导师，E-mail: xiasheng1519@163.com

作者简介: 高凤威（1995—），女，硕士研究生;

引用本文:

高凤威，江梦颖，张欣怡，吕云玮，姜戈晨，余宛君，刘烁，姚雪，蒋雅斓，陶泽华，邵启祥，夏圣. 黄芩苷对小鼠巨噬细胞干扰素基因刺激因子通路活化的影响[J]. 江苏大学学报:医学版, 2020, 30(05): 418-422. GAO Feng-wei， JIANG Meng-ying， ZHANG Xin-yi， Lv Yun-wei， JIANG Ge-chen， YU Wan-jun，.

Effects of Baicalin on the stimulator of interferon gene pathway activation in murine bone marrow-derived macrophages

. Journal of Jiangsu University(Medicine Edition), 2020, 30(05): 418-422.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2020/V30/I05/418

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