Tumor-associated macrophage impacts anti-tumor responses through activation of liver X receptors
(1.School of Clinical Medicine, Jiangsu University, Zhenjiang Jiangsu 212001; 2.Department of Endocrine, Xuhui District Central Hospital, Shanghai 200031； 3.Department of Clinical Laboratory， the First People′s Hospital of Zhengzhou， Zhengzhou Henan 450004,China）
Abstract：Objective:To investigate the connection between tumor microenvironment and liver X receptors(LXRs), and their function on the development of tumorassociated macrophage. Methods: Macrophages were sorted from tumor tissue and peritoneal lavage fluid, the expression level of LXRs, IL10 and IL12 were determined by realtime PCR. The internal relations among microenvironment , LXR receptors and TAM were explored in seperated experiments as follows: Macrophages cell line RAW264.7 were cocultured with tumor cell line TC1 ,or treated with tumor supernatant or LXR agonists , or the LXR signaling pathway in macrophages were blocked, then the phenotypes and functions of macrophages were determined. Results: Tumor microenvironment could promote the expression of LXR receptors, and induce the M2 subset phenotype of macrophages. The activation of LXR receptors could also induce the M2 phenotype, and the effect could be reversed when the LXR signaling pathway in macrophages were blocked. Conclusion: LXR could be activated by tumor microenvironment, then induce the accumulation of TAM, and activate their function on promote the growth, metastasis, and immune evasion of tumor.
引用本文:
钟晓敬1, 2, 王秀珍3, 盛宏光2. 肿瘤相关巨噬细胞肝X受体的激活对抗瘤应答的影响[J]. 江苏大学学报:医学版, 2012, 22(4): 302-306.
ZHONG Xiao-Jing-1, 2 , Wang-Xiu-Zhen-3, Sheng-Hong-Guang-2. Tumor-associated macrophage impacts anti-tumor responses through activation of liver X receptors. Journal of Jiangsu University(Medicine Edition), 2012, 22(4): 302-306.
2012, Vol. 22 
