Abstract：Objective: To investigate the protective effects of clodronate-liposome on cerebral damage induced by severe acute pancreatitis(SAP) in rats. Methods: Clodronateliposome was prepared by means of thin film. The models of SAP were performed by prinking injection of 5% sodium taurocholate (2 mL/kg) into the pancreatic capsule. A total of 48 male healthy SD rats were randomly divided into the sham group (Sham), SAP+liposome group (LB), SAP+ clodronate-liposome group (LC). LB group were injected with liposome (4 mL/kg) while LC group were injected with clodronateliposomes (4 mL/kg) in tail vein immediately after the SAP model performed. The change of amylase (AMS) in serum and the level of IL-6, TNF-α were obtained at 2 h and 6 h after operation, respectively. Pathologic alteration of pancreas was observed and pathological score of pancreatic tissue was also estimated. The water and Evan′s blue content of brain tissues, the content of myelin basic protein(MBP) in cerebrospinal fluid were detected. Results: Compared with Sham group, the serum levels of AMS, IL-6, TNF-α and pancreatic tissue pathological score were significant elevated at 2 and 6 hours in LB group（P<0.01）. In LC group, the serum levels of AMS, IL-6, TNF-α and pancreatic tissue pathological score were significant decreased at 2 and 6 hours compared with LB group（P<0.01）. Compared with Sham group, the water and Evan′s blue content of brain tissues, the content of MBP in cerebrospinal fluid were significant elevated at 2 and 6 hours in LB group（P<0.01）. In LC group, the water and Evan′s blue content of brain tissues, the content of MBP in cerebrospinal fluid were significant decreased at 2 and 6 hours compared with LB group（P<0.01）. Conclusion: Monocytes/macrophages can be depleted by phagocytosis of liposome encapsulated clodronate, which may contribute to protection of clodronate-liposome against cerebral damage caused by SAP in rats.
刘源, 李涛, 党胜春. 氯膦酸二钠脂质体对重症急性胰腺炎大鼠脑损伤的保护作用[J]. 江苏大学学报:医学版, 2016, 26(03): 219-.
LIU Yuan, Li-Tao, Dang-Sheng-Chun. Protective effects of clodronateliposome on cerebral damage induced by severe acute pancreatitis in rats. Journal of Jiangsu University(Medicine Edition), 2016, 26(03): 219-.
2016, Vol. 26 
