Gene chip analysis of synovial macrophages in rheumatoid arthritis
TONG Ye1， WU Zhen-fang2， WANG Yu-xiang2， XU Hai-dong2
(1. Faculty of Graduates， Bengbu Medical College， Bengbu Anhui 233000； 2. Department of Orthopedics, General Hospital of Eastern Theater Command， Nanjing Jiangsu 210002， China）
Abstract：Objective: To identify key genes of rheumatoid arthritis (RA) in order to better understand the underlying pathogenesis of RA. Methods: Differential expression genes (DEGs) in RA synovial macrophages were identified by comprehensive analysis of expression profiles. To explore the potential biological role of DEGs in RA, functional annotation, proteinprotein interaction (PPI) network construction and core gene search were used. The RNA expression level of DEGs in peripheral blood of RA was validated in GSE17755 dataset. Results: Compared with the control group, 489 DEGs were identified in synovial macrophages of RA patients, including 359 upregulated genes and 130 downregulated genes. DEGs are highly enriched in inflammatory response regulation, chemokine receptor binding and tumor necrosis factor signaling pathway. STAT1,CXCL10,FPR2,ITGAM,PPBP,IRF7,CCL5,OASL,OAS3 and IRF1 are the hub genes in the PPI network of DEGs. The expression of 9 core genes except FPR2 in the GSE17755 dataset was detected and found that the expression levels of STAT1, PPBP and OASL in peripheral blood cells of RA patients were significantly upregulated, while ITGAM, IRF7, CCL5 and IRF1 were downregulated. Conclusion: STAT1, OASL and other genes may play an important role in RA diseases.
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2019, Vol. 29 
