Abstract：Objective: To explore the level of myeloid-derived suppressor cells(MDSCs), Th17 and Treg cells in spleen cells of asthmatic mice. Methods: BALB/c male mice at age of 6-8 weeks were randomly divided into two groups: PBS group and model group. The group of asthmatic mice was established with OVA, and the ova-specific IgE in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA). HE staining and PAS staining were used to observe the pathological change in lung tissues. The levels of Th17, Treg and MDSCs in splenocyte were detected by flow cytometry. Immunohistochemical staining was used to observe the expression of Gr-1, IL-17 and Foxp3 in lung tissues of mice. Results: Compared with PBS group, airway inflammatory cells and mucus secretion were increased in the model group and serum IgE level was significantly increased. The proportion of Th17 cells in spleen cells increased and that of Treg cells decreased. The percentage of G-MDSCs subset in MDSCs was increased. Meanwhile, IL-17 and Gr-1 were highly expressed and Foxp3 was low in lung tissue of model group. Conclusion: High proportion of G-MDSCs and Th17 cells, and low proportion of Treg may be contributed to pathogenesis of asthmatic mice.
［1］Li C, Sheng A, Jia X, et al. Th17/Treg dysregulation in allergic asthmatic children is associated with elevated notch expression\[J\]. J Asthma, 2018, 55(1)：1-7.
［2］Limagne E, Euvrard R, Thibaudin M, et al. Accumulation of MDSC and Th17 cells in patients with metastatic colorectal cancer predicts the efficacy of a FOLFOXBevacizumab drug treatment regimen\[J\]. Cancer Res, 2016, 76(18)：5241-5252.
［3］Wen L, Gong P, Liang C, et al. Interplay between myeloidderived suppressor cells (MDSCs) and Th17 cells: foe or friend?\[J\]. Oncotarget, 2016, 7(23)：35490-35496.
［5］Zhou X, Hong J. Pediatric asthma management in China: current and future challenges\[J\]. Paediatr Drugs, 2018, 20(2)：105-110.
［6］Xu L, Li J, Zhang Y, et al. Regulatory effect of baicalin on the imbalance of Th17/Treg responses in mice with allergic asthma\[J\]. J Ethnopharmacol, 2017, 208：199-206.
［7］Anatriello E, Cunha M, Nogueira J, et al. Oral feeding of lactobacillus bulgaricus N45.10 inhibits the lung inflammation and airway remodeling in murine allergic asthma: relevance to the Th1/Th2 cytokines and STAT6/Tbet\[J\]. Cell Immunol, 2019, 341：103928.
［9］Arocena AR, Onofrio LI, Pellegrini AV, et al. Myeloidderived suppressor cells are key players in the resolution of inflammation during a model of acute infection\[J\]. Eur J Immunol, 2014, 44(1)：184-194.
［10］Glenn JD, Liu C, Whartenby KA. Frontline Science: Induction of experimental autoimmune encephalomyelitis mobilizes Th17promoting myeloid derived suppressor cells to the lung\[J\]. J Leukoc Biol, 2019, 105(5)：829-841.
［11］Wu H, Zhen Y, Ma Z, et al. Arginase1dependent promotion of Th17 differentiation and disease progression by MDSCs in systemic lupus erythematosus\[J\]. Sci Transl Med, 2016, 8(331)：331ra340.
［12］Wang Y, Tian J, Wang S. The potential therapeutic role of myeloidderived suppressor cells in autoimmune arthritis\[J\]. Semin Arthritis Rheum, 2016, 45(4)：490-495.
