Preparation of a new magnetic bone cement and its effect on apoptosis of MG-63 cells in an alternating magnetic filed
LIU Yi-hao1,2, YU-Xiang2, ZHANG Li2, CUI Xue-wen1, HE Shu-li2
（1. Department of Orthopaedics, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Department of Physics, Capital Normal University， Beijing 100048, China)
Abstract：Objective: To prepare a new magnetic bone cement,and to test the magnetic characterizations,setting time,compressive strengths ,heatgenerating abilities, biotoxicity and the ability of killing tumor cells in alternating magnetic fields. Methods: Prepared the magnetic nanoparticles via hightemperature organic phase decomposition,described the characterization,tested its composition and magnetic properties.Four types of magnetic cement,designated 0wt%,5wt%,10wt% and 15wt% were prepared.The compressive strength and setting time of the cement samples was measured according to the standard ISO 5833 procedure.The cement samples met the criterion listed in ISO 5833 were used to test the heatgenerating abilities in an alternating magnetic filed(390 kHz,22 kA/m) and evaluate the apoptosis of the MG63 cells by flow cytometry.The biotoxicity of the selected samples was examined with CCK8 after 24 h,48 h and 72 h respectively. Results: The prepared Co0.04Fe2.96O4 was superparamagnetic.The setting time was increased with the increased content of magnetic nanoparticles.Only compressive strengths of 0wt%\[(86.4±6.3)MPa\] and 5wt%\[(77.5±6.1)MPa\] samples met the ISO 5833 procedure. The temperature of cement samples containing 5wt% magnetic nanoparticles placed in an alternating magnetic filed(390 kHz,22 kA/m) for 3 min was up to 50 ℃ which was met the need of clinical thermal therapy. Cement samples containing 5wt% magnetic nanoparticles placed in an alternating magnetic filed(390 kHz,22 kA/m) for 6 min and at 50 ℃ for 30 min can cause 80.78% and 77.78% apoptosis. What′s more,the results between control groups and cement samples containing 5wt% magnetic nanoparticles showed no statistical significance (P>0.05). Conclusion: The setting time, compressive strengths and heatgenerating abilities of cement samples containing 5wt% magnetic nanoparticles met the need of clinical thermal therapy. The cement samples containing 5wt% magnetic nanoparticles placed in an alternating magnetic filed can induce MG63 cells apoptosis effectively. Acute heat generation in a short time is more effective than maintaining at 50 ℃ in a longer time. It was valuable for improving treatment strategies. In addition, cement samples containing 5wt% magnetic nanoparticles used in our studies also possessed the excellent biocompatibility which showed good prospects for clinical application.
刘义灏1,2， 俞翔2， 张力2， 崔学文1， 贺淑莉2. 一种新型磁性骨水泥的制备及其在交变磁场中对骨肉瘤MG-63细胞凋亡的影响[J]. 江苏大学学报:医学版, 2017, 27(01): 5-9.
LIU Yi-hao1,2, YU-Xiang2, ZHANG Li2, CUI Xue-wen1, HE Shu-li2. Preparation of a new magnetic bone cement and its effect on apoptosis of MG-63 cells in an alternating magnetic filed. Journal of Jiangsu University(Medicine Edition), 2017, 27(01): 5-9.
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2017, Vol. 27 
