Compare the effect of resistance to polymyxin B between virK and mig-14 gene of Salmonella enterica serovar Typhi
(1.Department of Clinical Laboratory, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract:Objective: To compare the effect and study the association in resistance to polymyxin B between virK and mig-14 gene of Salmonella enterica serovar Typhi. Methods: Prepared the virK deleted mutant and virK/ mig-14 double deleted mutant of S. enterica serovar Typhi by homologous recombination mediated by suicide plasmid;performed the polymyxin B sensitivity assay for wild strain,mig-14 deleted mutant,virK deleted mutant,virK/ mig-14 double deleted mutant and phoP deleted mutant,then compared their resistance to polymyxin B. Results: Successfully prepared a virK mutant and virK/mig-14 double mutant. The effect of polymyxin B on mig-14 mutant,virK mutant and virK/mig-14 double mutant were nearly similar at poor acid environment from the polymyxin B sensitivity assay.Conclusion: VirK participated in the regulations of resistance to polymyxin B. The resistance to polymyxin B of virK mutant was nearly consistent with mig-14 mutant.