Abstract:Objective: To establish experimental Sjgren syndrome(ESS) model by immunizing with salivary gland protein. Methods: C57BL/6 mice were immunized with salivary gland protein to establish ESS mouse model. The saliva flow rate was detected. Serum levels of autoantibodies was detected by ELISA kit. In addition, the pathological changes in salivary gland were observed by HE straining. Results: Compared with control group, the saliva flow rate was reduced in ESS mice(P<0.05), autoantibodies against salivary gland, antiM3R antibodies and antinuclear antibodies were significantly increased(all P<0.05). ESS mice exhibited bigger cervical lymph nodes and submandibular glands, and plenty of lymphocytes were infiltrated into salivary glands. Conclusion: The ESS model was successfully established by immunizing C57BL/6 mice with salivary gland protein.
收稿日期: 2019-05-06
基金资助:
江苏大学学生科研课题项目(17A483)
通讯作者:
王胜军(通讯作者),教授,博士生导师,E-mail:sjwsj@ujs.edu.cn
作者简介: 洪悦(1993—),女,硕士研究生
引用本文:
洪悦, 周惠敏, 田洁, 芮棵, 王胜军. 小鼠实验性干燥综合征模型的建立[J]. 江苏大学学报:医学版, 2019, 29(05): 390-393.
HONG Yue, ZHOU Hui-min, TIAN Jie, RUI Ke, WANG Sheng-jun. Establishment of experimental Sjogren syndrome mouse model. Journal of Jiangsu University(Medicine Edition), 2019, 29(05): 390-393.
[1]Voulgarelis M, Tzioufas AG. Pathogenetic mechanisms in the initiation and perpetuation of Sjgren′s syndrome\[J\]. Nat Rev Rheumatol, 2010, 6(9): 529-537.[2]Maciel G, Crowson CS, Matteson EL, et al. Incidence and mortality of physiciandiagnosed primary Sjgren syndrome: time trends over a 40year period in a populationbased US cohort\[J\]. Mayo Clin Proc, 2017, 92(5): 734-743.[3]Sun L, Akiyama K, Zhang H, et al. Mesenchymal stem cell transplantation reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans\[J\]. Stem Cells, 2009, 27(6): 1421-1432.[4]Papageorgiou A, Ziogas DC, Mavragani CP, et al. Predicting the outcome of Sjgren′s syndromeassociated nonhodgkin′s lymphoma patients\[J\]. PLoS One, 2015,10(2): e0116189.[5]Gibbs RA, Weinstock GM, Metzker ML, et al. Genome sequence of the Brown Norway rat yields insights into mammalian evolution\[J\]. Nature, 2004, 428(6982): 493-521.[6]Lin X, Song JX, Shaw PC, et al. An autoimmunized mouse model recapitulates key features in the pathogenesis of Sjgren′s syndrome\[J\]. Int Immunol, 2011, 23(10): 613-624.[7]Gottenberg JE, Seror R, MiceliRichard C, et al. Serum levels of β2microglobulin and free light chains of immunoglobulins are associated with systemic disease activity in primary Sjgren′s syndrome. Data at enrollment in the prospective assess cohort\[J\]. PLoS One, 2013, 8(5): e59868.[8]Pearson JA, Wong FS, Wen L. The importance of the Non Obese Diabetic(NOD) mouse model in autoimmune diabetes\[J\]. J Autoimmun, 2016(66): 76-88.[9]Park YS, Gauna AE, Cha S. Mouse models of primary Sjogren′s syndrome\[J\]. Curr Pharm Des, 2015, 21(18): 2350-2364.[10]MaierMoore JS, Kurien BT, D′souza A, et al. Passive transfer of antibodies to the linear epitope 60 kD Ro 273-289 induces features of Sjgren′s syndrome in naive mice\[J\]. Clin Exp Immunol, 2015, 180(1): 19-27.[11]Kaminski B. Laryngological manifestations of Sjogren′s syndrome\[J\]. Reumatologia, 2019, 57(1): 37-44.