目的: 比较6对通用引物检测9种不同型别人乳头瘤病毒（HPV）的效果，为临床提供一种快速简便的HPV检测方法。方法: 收集女性宫颈脱落细胞标本954例，使用HPV分型试剂盒进行HPVDNA检测并分型。分别使用6对通用引物（① GP5/GP6，② GP5+/GP6+，③ CPⅠ/CPⅡS，④ CPI/CPⅡG，⑤ 外：MY09/MY11，内：GP5+/GP6+，⑥ SPF1/GP6++）对HPV-DNA阳性标本进行PCR检测，比较6对通用引物检测9种不同类型HPVDNA效果。结果： 采用HPV分型试剂盒从954例标本中共检出263例HPV-DNA阳性标本，阳性率为27.57%。在阳性标本中，采用通用引物SPF1/GP6++检出的阳性例数为223例，检出率为84.79%；采用巢氏PCR引物（外：MY09/MY11，内：GP5+/GP6+）检出的阳性例数为207例，检出率为78.71%。上述两对通用引物均可以检出9种型别HPVDNA。结论： 通用引物SPF1/GP6++检测效果最佳，但引物含次黄嘌呤，成本较高；巢氏PCR引物（外：MY09/MY11，内：GP5+/GP6+）检测效果次之，且合成成本较低。在HPV研究中可以根据实际情况选用。
Abstract
Objective: To compare the detection efficacy of 6 universal primers in detecting 9 different types of human papillomavirus (HPV) and provide a quick and easy screening method for clinical studies. Methods: We collected 954 female cervical exfoliated cell samples, then detected and classified the HPVDNA with HPV typing kit. Then we used 6 pairs of universal primers （①GP5/GP6，②GP5+/GP6+，③CP-Ⅰ/CP-ⅡS，④CP-I/CP-ⅡG，⑤external：MY09/MY11，internal：GP5+/GP6+，⑥SPF1/GP6++）to detect HPVDNA positive samples by PCR and compared their detection effect in 9 types of HPV-DNA. Results: A total of 263 HPV-DNA positive samples were detected from 954 specimens with HPV typing kit， the HPV infection rate was 27.57%. In the HPVDNA positive samples, the positive cases detected by SPF1/GP6++ was 223, and the detection rate was 84.79%. But 207 positive cases were detected by nested PCR primers (external: MY09/MY11, internal: GP5+/GP6+), the corresponding detection rate was 78.71%. The two universal primers above were able to detect all 9 HPVDNA types. Conclusion: The universal primer SPF1/GP6++ has the best detection efficacy, but the synthesis cost is higher because of containing hypoxanthine. The nest PCR primer (external: MY09/MY11, inner:GP5+/GP6+) has the second highest detection efficacy, and the synthesis cost is lower. Therefore, we may select suitable universal primers according to the actual situation in HPV detection.
关键词
人乳头瘤病毒 /
通用引物 /
HPV基因型 /
PCR
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参考文献
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脚注
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基金
江苏省2018年度预防医学科研课题（Y2018108）; 镇江市社会发展项目（SH2017024）
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