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Journal of Jiangsu University(Medicine Edition)
 
2020 Vol.30 Issue.04
Published 2020-07-30
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2020 Vol. 30 (04): 1- [Abstract] ( 36 ) [HTML 1KB] [ PDF 390KB] ( 460 )
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Human umbilical cord mesenchymal stem cell-derived exosomes promote microglial polarization to M2
Human umbilical cord mesenchymal stem cell-derived exosomes promote microglial polarization to M2[J]. Journal of Jiangsu University(Medicine Edition), 2020,20(04): 277-281>')" href="#"> ZHOU Xin-ru1, JIN Qian1, ZHANG Lei-lei1, WU Pei-pei1, FU Qiang2, QIAN Hui1
Objective: To investigate the role and mechanism of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Ex) in polarized transformation of microglia, and to provide a new treatment for clinical treatment of neurodegenerative diseases related to neuroinflammation. Methods: Primary human umbilical cord mesenchymal stem cells were isolated and cultured, and the conditioned medium was collected to extract exosomes. Electron microscopy, nanoparticle tracking analysis(NTA) technology and Western blotting technology were used to characterize their particle size, morphological characteristics and surface markers. Microglial cell lines were treated with PBS, LPS (1 μg/mL), LPS (1 μg/mL)+hucMSC-Ex (100 μg/mL) for 12 h, respectively.The expression of calcium-binding protein Iba1, a specific marker, was detected by immunofluorescence in different groups; Western blotting was used to detect changes of M1 and M2 microglia markers in different groups and to screen for possible signaling mechanisms; qRT-PCR was used to detect the changes of IL-1β mRNA and IL-10 mRNA in microglial cells in different groups; ELISA was used to detect the secretion of cytokines IL-1β and IL-10 in cell conditioned medium. Results: The electron microscope showed a typical membrane “cup and plate” structure, NTA results showed that the diameter of hucMSC-Ex was（119.7±47.9）nm and Western blotting confirmed that it expressed characteristic CD9, CD63, and CD81 surface molecules; immunofluorescence results showed that calcium-binding protein Iba1 in the activated group (LPS group, LPS + hucMSCEx group), was higher than the resting group (PBS group), and the LPS + hucMSC-Ex group was higher than the LPS group; Western blotting combined with qRTPCR results showed that hucMSC-Ex may reduce the expression of M1 microglia markers iNOS and IL-1β, and increase the expression of M2 microglia markers Arg1 and IL-10 by inhibiting NF-κB activation; ELISA results showed that compared with the LPS group, the inflammatory factor IL-1β decreased and the anti-inflammatory factor IL-10 increased in the LPS+hucMSC-Ex group. Conclusion: hucMSC-Ex may promote the polarization of microglia to M2 by inhibiting NF-κB activation and reduce the inflammatory response.
2020 Vol. 30 (04): 277-281 [Abstract] ( 37 ) [HTML 1KB] [ PDF 4578KB] ( 534 )
282
The inhibition of umbilical cord mesenchymal stem cell-derived exosomes on  rat renal interstitial fibrosis
The inhibition of umbilical cord mesenchymal stem cell-derived exosomes on  rat renal interstitial fibrosis[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 282-286>')" href="#"> HU Yu-yan，YIN Lei，ZHANG Chen-xiao，QIAN Hui
Objective: To investigate the repair effect of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Ex) on renal tubular interstitial fibrosis induced by unilateral ureteral obstruction (UUO) in rats. Methods: Human umbilical cord-derived mesenchymal stem cells were isolated and cultured, and hucMSC-Ex was extracted from the culture supernatant by ultrafiltration and gradient centrifugation. A unilateral ureteral ligation method was used to construct a renal interstitial fibrosis model. All rats were randomly divided into sham operation group， UUO group and hucMSC-Ex treatment group. Two hundred-fifty μg hucMSC-Ex was injected into the tail vein after the operation of treatment group rats. Seven days later, kidney tissues were collected and the general appearance of the kidneys in each group was observed. Western blotting was used to detect the expression of protein related to renal fibrosis: TGF-β1, E-cadherin and Vimentin. The kidney tissue sections were stained with HE staining, Masson staining and immunohistochemical staining to measure rat renal pathological changes, inflammatory cell infiltration and the degree of renal fibrosis in the obstructed rats. Results: The inflammatory cell infiltration in the kidney of UUO group significantly increased, the tissue structure was severely damaged, and extensive collagen deposition was observed. After hucMSC-Ex treatment, the tissue morphology and structure of were partially recovered, collagen deposition and inflammatory cell infiltration were reduced. At the same time, the expression of α-SMA, TGF-β1 and Vimentin in the kidney of UUO group increased, while the expression of E-cadherin decreased. However, hucMSC-Ex treatment could inhibit the expression of α-SMA and TGF-β1 and reverse the epithelial-mesenchymal conversion. Conclusion: hucMSC-Ex can reduce the pathological damage of renal tissue in rats and delay the progress of renal interstitial fibrosis in rats.
2020 Vol. 30 (04): 282-286 [Abstract] ( 49 ) [HTML 1KB] [ PDF 5528KB] ( 483 )
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Regulation of oxidative stress by exosomes derived from mesenchymal stem cells protects against D-galactosamine hydrochloride/lipopolysaccharides induced acute liver injury
Regulation of oxidative stress by exosomes derived from mesenchymal stem cells protects against D-galactosamine hydrochloride/lipopolysaccharides induced acute liver injury[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 287-292>')" href="#"> ZHAO Jia-wei1,2, SHI Min2， ZHENG Jin-xu3
Objective: To explore the therapeutic effect and possible mechanism of exosomes derived from mouse mesenchymal stem cells(MSC) on Dgalactosamine hydrochloride/lipopolysaccharides induced acute liver injury. Methods: Mouse mesenchymal stem cells were extracted and detected by flow cytometry and cell differentiation experiment. The exosomes of MSC were collected and purified by ultracentrifugation and identified by transmission electron microscopy and Western blotting. Size distribution and zeta potential of exosomes were measured by NTA. The ability of exosomes to reduce the oxidative damage was detected by reactive oxygen species(ROS) fluorescence probe. CCK-8 assay was used to investigate the protective effect of exosomes on hepatocytes. The therapeutic effect of exosomes on D-GalN/LPS-induced ALI was also evaluated. Results: Primary mesenchymal stem cells expressed CD29 and CD44 but did not express CD31 and CD117. The exosomes derived from mesenchymal stem cells were nano-sized vesicles with typical cup-shaped structure, which expressed CD9 and CD63. Compared with PBS group, exosomes could reduce the level of ROS and improve the viability of L02 cells, while exosomes themselves had no obvious effect on cell viability. Compared with PBS group, exosomes could reduce liver damage and transaminase level, regulate the levels of malondialdehyde, catalase and superoxide dismutase. Conclusion: At the cellular level, exosomes derived from mesenchymal stem cells could reduce ROS level and protect hepatocyte viability. At the animal level, exosomes could down regulate the expression of oxidative stressrelated markers and promote liver tissue repair.
2020 Vol. 30 (04): 287-292 [Abstract] ( 75 ) [HTML 1KB] [ PDF 2667KB] ( 534 )
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2020 Vol. 30 (04): 293-297,301 [Abstract] ( 37 ) [HTML 1KB] [ PDF 352KB] ( 478 )
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2020 Vol. 30 (04): 298-301 [Abstract] ( 50 ) [HTML 1KB] [ PDF 333KB] ( 497 )
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Protective effect of human neural stem cell microvesicles against glutamateinduced cytotoxicity in PC12 cells
Protective effect of human neural stem cell microvesicles against glutamateinduced cytotoxicity in PC12 cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 302-306>')" href="#"> YU Jia-hong, TONG Yu, YE Kai, CHEN Tian-yan, TANG Bin, HU Jia-bo
Objective: To investigate the effect of human neural stem cell microvesicles (hNSCMVs) against glutamate-induced cytotoxicity in PC12 cells and its possible mechanism. Methods: Excitative injury model was established by treating rat adrenal medulla pheochromocytoma PC12 cells with glutamate. Experiment was divided into three groups: control group, PC12 cells without any treatment; glutamate group, PC12 cells treated with glutamate for 24 h; hNSC-MVs + glutamate group, PC12 cells were pretreated with 200 mg/L hNSC-MVs for 24 h, and then treated with glutamate for 24 h. MTT method was used to detect the survival rate of PC12 cells and Western blotting was used to determine the expression of apoptosis-related proteins, Bax and Bcl-2. Results: PC12 cells could internalize hNSC-MVs. Glutamate has a toxic effect on PC12 cells, and its half inhibitory concentration is 25 mmol/L. Compared with glutamate group, the survival rate of PC12 cells in hNSC-MVs + glutamate group was surprisingly increased(P<0.01). Compared with control group, the expression of Bax protein in glutamate group was markedly increased, and the expression of Bcl-2 protein was greatly reduced. Compared with glutamate group, the expression of Bax protein in hNSC-MVs + glutamate group was significantly down-regulated, and the expression of Bcl-2 protein was remarkably up-regulated(all P<0.01). Conclusion: hNSC-MVs may play a protective role by inhibiting glutamate-induced PC12 cell apoptosis.
2020 Vol. 30 (04): 302-306 [Abstract] ( 47 ) [HTML 1KB] [ PDF 2105KB] ( 407 )
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Human umbilical cord mesenchymal stem cells-derived extracellular vesicles promote proliferation and migration of schwann cells
Human umbilical cord mesenchymal stem cells-derived extracellular vesicles promote proliferation and migration of schwann cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 307-311>')" href="#"> ZHANG Huan-yan1,2， MA Yong-bin2， DONG Li-yang1， ZHANG Wen-zhe1，XUE fei1， SHAN Wen-qi1， WANG Xue-feng1
Objective: To investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation and migration of schwann cells. Methods: EVs was isolated and purified from supernatant of hUCMSCs by ultrahigh speed centrifugation, morphological characteristics of EVs were observed by transmission electron microscope. Western blotting was used to detect the expression of EVs marker proteins CD63 and CD9. The effects of different concentrations of hUCMSCEVs on proliferation and migration of schwann cells were tested by CCK8 and Transwell chamber experiments. Immunofluorescence assay was used to observe the fusion of EVs and primary schwann cells cell membranes. Results: hUCMSCEVs was round or oval and expressed specific molecular markers CD63 and CD9; Compared with the control group, hUCMSCEVs significantly promoted schwann cells proliferation (P<0.05) and migration (P<0.05). hUCMSCEVs fuse with cell membrane after incubation with schwann cells. Conclusion: hUCMSCEVs can significantly promote proliferation and migration of schwann cells in a concentrationdependent manner.
2020 Vol. 30 (04): 307-311 [Abstract] ( 40 ) [HTML 1KB] [ PDF 2047KB] ( 442 )
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Expression and clinical significance of pseudogenes and long non-coding RNAs in the plasma of patients with renal clear cell carcinoma
Expression and clinical significance of pseudogenes and long non-coding RNAs in the plasma of patients with renal clear cell carcinoma[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 312-319>')" href="#"> ZOU Yuan-zhang, HE Yi-chen, CHEN Bing-hai
Objective: To investigate the expression and clinical significance of pseudogenes and long non-coding RNAs (lncRNAs) in the plasma of patients with renal clear cell carcinoma (RCC). Methods: Plasma samples were collected from 50 patients with RCC and 26 healthy subjects. qRT-PCR assay was used to detect the expression level of pseudogenes and lncRNAs in the plasma samples.The relationship between differentially expressed pseudogenes and lncRNAs and the prognosis of patients was analyzed based on cBioPortal, GEPIA, oncolnc, UALCAN, and Kaplan-Meier plotter databases. Target genes were up-regulated by MPH-dCase9-mediated transcription regulation in RCC cell line 786-O. Then their expression levels were validated by qRT-PCR, and CCK-8 assay was performed to measure the cell proliferation activity after the up-regulation of target genes. StarBase database was used to predict the miRNA targets of the target genes and analyze their expression in RCC. The relationship between miRNA targets and the prognosis of patients was analyzed in the KaplanMeier plotter database. Results: The expression levels of pseudogene PLEKHA8P1, CXCR2P1, KLRAP1, FER1L4, PDXDC2P and lncRNA TLX1NB, DIO3OS in the plasma of patients with RCC were obviously higher than that of healthy subjects, while the expression of LINC00482 displayed the opposite result. Pseudogenes PLEKHA8P1, CXCR2P1, FER1L4, and lncRNA TLX1NB, LINC00482 as well as DIO3OS were mutated at different frequencies in RCC and were relevant to the overall survival of patients. Among them, CXCR2P1, FER1L4, and TLX1NB were also related to the disease-free survival of patients. In addition, high expression of PLEKHA8P1, KLRAP1, PDXDC2P, FER1L4, and DIO3OS were associated with poor prognosis of RCC patients, while the expression of LINC00482 was no significance in overall survival. In addition low expression of TLX1NB was related to poor prognosis, which was contrary to the expectation. Analysis of RCC samples from TCGA database demonstrated that FER1L4 and DIO3OS were high expression in RCC tissues when compared with normal tissues. The results of in vitro experiment indicated that FER1L4 and DIO3OS up-regulation could promote the proliferation of 786-O cells. We identified 24 and 13 predicted miRNA targets for FER1L4 and DIO3OS from StarBase database, respectively. Of which the expression of hsa-miR-556-5p and hsa-miR-660-5p were negatively correlated with FER1L4 and DIO3OS, respectively. Additionally, they were also associated with poor prognosis of patients with RCC. Conclusion: The pseudogene PLEKHA8P1, CXCR2P1, KLRAP1, FER1L4, PDXDC2P and lncRNA DIO3OS may serve as potential biological indicators for early diagnosis and prognosis of RCC, of which FER1L4 and DIO3OS may be involved in the development of RCC via regulating hsa-miR-556-5p and hsa-miR-660-5p.
2020 Vol. 30 (04): 312-319 [Abstract] ( 41 ) [HTML 1KB] [ PDF 2562KB] ( 457 )
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Effect of autophagy core protein ATG101 on the differentiation and thermogenesis of beige adipocytes
Effect of autophagy core protein ATG101 on the differentiation and thermogenesis of beige adipocytes[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 320-325>')" href="#"> MA Jing-yuan, LI Shao-bo, YANG Di, BAI Ning-ning, Miriayi ·Alimujiang, YANG Ying, HAN Jun-feng
Objective: To explore the effects of autophagy related 101 (ATG101) on the proliferation, differentiation and thermogenesis of beige adipocytes. Methods: Mouse stromal stromal cells (SVF) were used to induce differentiation into beige adipocytes. Real-time quantitative PCR and Western blotting were used to detect the expression of Atg101 mRNA and protein after induction(0, 2, 4, 6 and 8 days). Lentiviral-mediated shRNA was used to knock down Atg101 before differentiation and then induced differentiation into beige adipocytes, then real-time quantitative PCR was used to detect the expression of marker genes in mature adipocytes (6 d) and to observe the morphology of lipid droplets, as well as the expression of genes related to thermogenesis during differentiation (0, 2, 4, and 6 d). Results: The expression of ATG101 increased gradually during the natural differentiation of beige adipocytes. Compared with the negative control group, the expression of mature beige adipocyte marker genes fatty acid binding protein 4 (Fabp4), cell death-inducing DNA fragmentation factor-α-like effector A (Cidea) and  glucose transporter 4 (Glut4) was significantly reduced (all P<0.05), lipid droplet formation was impaired. At the same time, the knockdown of Atg101 significantly reduced the expression of thermogenesis genes PR domaincontaining 16 (Prdm16), Cidea and uncoupling protein 1 (Ucp1) in beige adipocytes (all P<0.05). Conclusion: ATG101 is involved in the differentiation of beige adipocytes and the downregulation of ATG101 can affect thermogenesis.
2020 Vol. 30 (04): 320-325 [Abstract] ( 63 ) [HTML 1KB] [ PDF 1213KB] ( 534 )
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Expression of miR133b-3p in the rat model of cerebral ischemia-reperfusion and its significance
Expression of miR133b-3p in the rat model of cerebral ischemia-reperfusion and its significance[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 326-329>')" href="#"> XU-Chen, LIU Jing-li
Objective: To analyze the expression of miR133b-3p and its role in the rat model of cerebral ischemiareperfusion. Methods: Twentyeight adult male SD rats were randomly divided into two groups: cerebral ischemiareperfusion group(n=14) and sham group(n=14). The occlusion model of middle cerebral artery was constructed by thread thrombus. The thread thrombus was removed 2 hours later. Only muscle was separated and blood vessels were exposed in sham group. After 24 hours, neurobehavioral score was used to evaluate the severity of cerebral infarction; TTC staining was used to measure the volume of cerebral infarction; TUNEL staining was used to observe the number of apoptotic cells in paraffin section of brain tissue; real-time fluorescence quantitative PCR was used to analyze the expression of miR133b-3p. Bioinformatics analysis was used to find the target gene related to apoptosis, and the interaction between miR133b3p and its target gene was verified by double luciferase system. Results: Compared with the sham group, the neurobehavioral score of cerebral ischemiareperfusion group increased significantly (Z=-4.365, P<0.01), the percentage of cerebral infarction volume increased, the number of apoptotic cells increased markedly (t=-4.232, P<0.05), and the expression of miR133b-3p decreased greatly (P<0.01). Bioinformatical analysis found that the possible target of miR133b-3p related to apoptosis was bcl2l2. The double luciferase target verification report indicated that the relative fluorescence intensity of bcl2l2 wild type decreased to some extent after miR133b3p transfection. Conclusion: The expression of miR133b-3p is significantly decreased in the rat model of cerebral ischemia-reperfusion injury, and bcl2l2 may be the downstream target gene.
2020 Vol. 30 (04): 326-329 [Abstract] ( 31 ) [HTML 1KB] [ PDF 635KB] ( 472 )
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Silencing DLL3 inhibits proliferation and migration of small cell lung cancer cell line H592
Silencing DLL3 inhibits proliferation and migration of small cell lung cancer cell line H592[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 330-335>')" href="#"> ZHOU Jing1, GUO Li2
Objective: To explore the effect of the silencing DLL3 (delta-like ligand 3) on the proliferation and migration of small cell lung cancer(SCLC).Methods: Clinical information of 50 patients with SCLC was collected from Shanxi Province Tumor Hospital, and the expression of DLL3 in cancer tissues was detected by immunohistochemical staining. The mRNA levels of DLL3 in H592 of SCLC cells were detected by real-time fluorescence quantitative PCR. si-RNA was used to silence the expression of DLL3 and MUC-1 genes in H592 cells, respectively. The expression of Mucins-1 (MUC-1) was detected by Western blotting after DLL3 silencing. Cell proliferation, apoptosis and migration were detected by CCK-8, flow cytometry and Transwell assay, respectively. Results: The positive expression of DLL3 in SCLC was 72%. The expression of DLL3 in carcinoma tissues was significantly higher than that in paracancer tissues (P<0.01). DLL3 was highly expressed in H592 cells (P<0.01). After the silencing of DLL3, the proliferation and migration ability of H592 cells were reduced and the apoptotic ability was enhanced. In addition, the intracellular expression level of MUC-1 protein was decreased. Pearson correlation analysis showed that there was a significant positive correlation between the expression levels of DLL3 and MUC-1 (r=0.83, P<0.01). After the silencing of MUC-1, the proliferation and migration ability of H592 cells were reduced and the apoptotic ability was enhanced. Conclusion: DLL3 and MUC-1 were highly expressed in SCLC, and silencing of DLL3 and MUC-1 can inhibit the proliferation and migration of cancer cells and promote apoptosis.
2020 Vol. 30 (04): 330-335 [Abstract] ( 56 ) [HTML 1KB] [ PDF 3473KB] ( 498 )
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#br# Effect of tetrandrine on human colorectal cancer HCT116 cell line and its potential mechanism
#br# Effect of tetrandrine on human colorectal cancer HCT116 cell line and its potential mechanism[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 336-341>')" href="#"> WANG Yue-xia1, ZHANG Zhi-jin2, ZHANG Yu-hao2, FU Jun2, QIN Xian-ju2
Objective: To explore the effect of tetrandrine (TET) on human colorectal cancer HCT116 cell line and its potential mechanism. Methods: CCK8 assay was used to assess cells in viability and its 50% inhibitory concentration following treatment with TET. HCT116 cells were treated with different concentrations of TET (0, 2.5, 5 and 10 μmol/L), and cell colony formation assay was used to detect cell proliferation. Cell apoptosis was determined by using flow cytometry, mitochondrial membrane potential (MMP) assay and Hoechst 33342 fluorescent staining, and cell migration was determined by migration assay and Wound healing assay. The protein expression level of epithelial-mesenchymal transition marker protein and PI3K/AKT/NF-κB signal pathway after TET treatment was determined by Western blotting. Results: The activity of HCT116 cells decreased with the increase of TET concentration, and the 50% inhibition concentration of TET was 9.441 μmol/L. Compared with 0 μmol/L group, the number of colony forming cells in 2.5, 5 and 10 μmol/L TET groups decreased (all P<0.05), and the proportion of cells in G0/G1 phase increased, the proportion of cells in S phase and G2/M phase decreased in 5 and 10 μmol/L TET groups(all P<0.05). Compared with 0 μmol/L group, 2.5, 5 and 10 μmol/L TET group had more apoptosis and less migration ability (all P<0.05), and cells appeared round in 10 μmol/L TET group. Compared with 0 μmol/L group, the expression of Ecadherin was increased in 2.5, 5 and 10 μmol/L TET groups， while N-cadherin and Vimentin expression were decreased, and the phosphorylation of AKT and NFκB (p65), the key molecules in PI3K/AKT/NF-κB signaling pathway was inhibited(all P<0.05). Conclusion: TET may reverse the epithelial-mesenchymal transformation of HCT116 cells by inhibiting PI3K/Akt/NFκB signal pathway, and then reduce the ability of cell proliferation and migration and induce apoptosis.
2020 Vol. 30 (04): 336-341 [Abstract] ( 58 ) [HTML 1KB] [ PDF 5127KB] ( 446 )
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Bioinformatics analysis of differential methylation of DNA and actin alpha 2 in nasal epithelium cells of asthma patients
Bioinformatics analysis of differential methylation of DNA and actin alpha 2 in nasal epithelium cells of asthma patients[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 342-346>')" href="#"> ZHANG Li-sha, QIAN Fen-hong
Objective: To investigate the relationship between asthma and the differences of DNA methylation in nasal epithelium cells and to detect the methylation level of actin alpha 2(ACTA2) in asthma patients. Methods: Nasal epithelium cell samples from 6 asthma patients and 5 healthy adults were collected for the detection of methylation levels of whole genome by Illumina Infinium MethylationEPIC BeadChip. ACTA2 gene was screened out after the analysis of Gene Ontology function. Then, samples which were collected from 12 asthma patients and 12 healthy adults were detected by bisulfite genomic sequencing PCR to analyse the methylation level of ACTA2 promoter. Results: Illumina BeadChip illustrated that methylation changes were detected in all euchromosomes, most of which occured in gene body or intergenic region, only 10%-20% found in promoter. Meanwhile, hypermethylation and hypomethylation changes were found in the same chromosome; methylation level of ACTA2 promoter was higher in asthma patients than healthy adults(P=0.001). Conclusion: Asthma patients showed genomewide methylation level in nasal epithelium cells; and higher methylation level of ACTA2 promoter.
2020 Vol. 30 (04): 342-346 [Abstract] ( 68 ) [HTML 1KB] [ PDF 1348KB] ( 461 )
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Relationship between serum uric acid and glomerular filtration rate in patients with impaired fasting glucose
Relationship between serum uric acid and glomerular filtration rate in patients with impaired fasting glucose[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 347-350>')" href="#"> SHI Li1,2, LIU Xue-kui2,3, GONG Ying2, SANG Yi-quan2, LIANG Jun2,3
Objective: To investigate the correlation between serum uric acid and glomerular filtration rate (GFR) in patients with impaired fasting glucose（IFG） in community population, and to evaluate the effect of interaction between fasting blood glucose and serum uric acid on GFR. Methods: The subjects were 11 078 people who had physical examination in Xuzhou Central Hospital in 2016. They were divided into normal blood glucose group (3.8-6.0 mmol/L) and IFG group (6.1-7.0 mmol/L). The blood pressure, BMI, blood lipid and GGT were compared between the two groups. The estimated glomerular filtration rate (eGFR) was estimated using the MDRD formula corrected by the data of Chinese patients with chronic kidney disease. The correlation between serum uric acid and eGFR in patients with IFG was analyzed by univariate linear regression. Results: With the increase of serum uric acid, the eGFR of IFG patients showed a decreasing trend with the trend P value<0.001. After adjusting for sex, age, BMI, blood pressure, blood lipid and other indicators, the eGFR still decreased with the increase of serum uric acid, and the trend was statistically significant(P<0.001). There was an interaction between fasting blood glucose level and serum uric acid on the changes of eGFR（P=0.035）. Conclusion: Serum uric acid level is an independent risk factor of eGFR in IFG patients, and can interact with fasting blood glucose level to further reduce eGFR.
2020 Vol. 30 (04): 347-350 [Abstract] ( 71 ) [HTML 1KB] [ PDF 314KB] ( 490 )
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The feasibility of CT-based radiomic nomogram for predicting histological classification of gastric cancer with different prognosis
The feasibility of CT-based radiomic nomogram for predicting histological classification of gastric cancer with different prognosis[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(04): 351-356>')" href="#"> CHEN Jian1,2, HUANG Hai-xia2, LU Chao3, WANG Xiao-xiao3, DING Yi1, SHAN Xiu-hong3
Objective: To explore the application value of CTbased radiomic nomogram in predicting histological classification of gastric cancer with different prognosis. Methods: A total of 570 patients with gastric cancer were analyzed retrospectively，and divided into poor prognosis group and good prognosis group. All the samples were randomly divided into training cohort and validation cohort in the proportion of 2 ∶1. Standardized CT images were segmented by radiologists with ITKSNAP software manually.The clinical features with the greatest correlation with histological classification of gastric cancer were screened by univariate analysis, and used as parameters to construct the clinical feature model. Features are extracted from all segmented images, and minimum Redundancy Maximum Relevance (mRMR), Least Absolute Shrinkage and Selection Operator (LASSO) regression and stepwise regression were used to screen the redundant radiomic features. The effective characteristics were used to establish radiomic signature.Finally, the clinical features closely related to histological classification were fitted, and radiomic nomogram was constructed.The performance of each model was evaluated by the area under ROC curve (AUC). Results: A total of 985 features were extracted from all the segmentation region, which included first order statistics , shape features, texture features, wavelet features and other filtering feature. After the screening of redundant features, five key features were obtained, which were used as the parameters of radiomic signature. Three clinical characteristics (age, sex, CTM) were selected as the parameters of the clinical feature model. Compared with other two models (clinical feature model and radiomic signature), the classification performance of radiomic nomogram is the best.The AUC of radiomic nomogram in training cohort and validation cohort was 0.726 2 (95%CI:0.676-0.776) and 0.707 0(95%CI:0.632-0.781). Conclusion: CT-based radiomic nomogram has certain potentiality in noninvasive predicting different prognostis related to histological classification of gastric cancer before operation.
2020 Vol. 30 (04): 351-356 [Abstract] ( 61 ) [HTML 1KB] [ PDF 627KB] ( 461 )
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2020 Vol. 30 (04): 357-360 [Abstract] ( 36 ) [HTML 1KB] [ PDF 314KB] ( 614 )
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2020 Vol. 30 (04): 361-363 [Abstract] ( 26 ) [HTML 1KB] [ PDF 279KB] ( 530 )
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2020 Vol. 30 (04): 364-368 [Abstract] ( 40 ) [HTML 1KB] [ PDF 340KB] ( 525 )
江苏大学学报:医学版
　

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