自噬核心蛋白ATG101对米色脂肪细胞分化和产热的影响

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Abstract Objective: To explore the effects of autophagy related 101 (ATG101) on the proliferation, differentiation and thermogenesis of beige adipocytes. Methods: Mouse stromal stromal cells (SVF) were used to induce differentiation into beige adipocytes. Real-time quantitative PCR and Western blotting were used to detect the expression of Atg101 mRNA and protein after induction(0, 2, 4, 6 and 8 days). Lentiviral-mediated shRNA was used to knock down Atg101 before differentiation and then induced differentiation into beige adipocytes, then real-time quantitative PCR was used to detect the expression of marker genes in mature adipocytes (6 d) and to observe the morphology of lipid droplets, as well as the expression of genes related to thermogenesis during differentiation (0, 2, 4, and 6 d). Results: The expression of ATG101 increased gradually during the natural differentiation of beige adipocytes. Compared with the negative control group, the expression of mature beige adipocyte marker genes fatty acid binding protein 4 (Fabp4), cell death-inducing DNA fragmentation factor-α-like effector A (Cidea) and glucose transporter 4 (Glut4) was significantly reduced (all P<0.05), lipid droplet formation was impaired. At the same time, the knockdown of Atg101 significantly reduced the expression of thermogenesis genes PR domaincontaining 16 (Prdm16), Cidea and uncoupling protein 1 (Ucp1) in beige adipocytes (all P<0.05). Conclusion: ATG101 is involved in the differentiation of beige adipocytes and the downregulation of ATG101 can affect thermogenesis.

Received: 27 February 2020

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	Articles by authors
	MA Jing-yuan
	LI Shao-bo
	YANG Di
	BAI Ning-ning
	Miriayi ·Alimujiang
	YANG Ying
	HAN Jun-feng

Cite this article:

MA Jing-yuan,LI Shao-bo,YANG Di等.

Effect of autophagy core protein ATG101 on the differentiation and thermogenesis of beige adipocytes

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 320-325.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/320

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