粉防己碱对人结直肠癌HCT116细胞株生物功能的影响及其机制

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Abstract Objective: To explore the effect of tetrandrine (TET) on human colorectal cancer HCT116 cell line and its potential mechanism. Methods: CCK8 assay was used to assess cells in viability and its 50% inhibitory concentration following treatment with TET. HCT116 cells were treated with different concentrations of TET (0, 2.5, 5 and 10 μmol/L), and cell colony formation assay was used to detect cell proliferation. Cell apoptosis was determined by using flow cytometry, mitochondrial membrane potential (MMP) assay and Hoechst 33342 fluorescent staining, and cell migration was determined by migration assay and Wound healing assay. The protein expression level of epithelial-mesenchymal transition marker protein and PI3K/AKT/NF-κB signal pathway after TET treatment was determined by Western blotting. Results: The activity of HCT116 cells decreased with the increase of TET concentration, and the 50% inhibition concentration of TET was 9.441 μmol/L. Compared with 0 μmol/L group, the number of colony forming cells in 2.5, 5 and 10 μmol/L TET groups decreased (all P<0.05), and the proportion of cells in G0/G1 phase increased, the proportion of cells in S phase and G2/M phase decreased in 5 and 10 μmol/L TET groups(all P<0.05). Compared with 0 μmol/L group, 2.5, 5 and 10 μmol/L TET group had more apoptosis and less migration ability (all P<0.05), and cells appeared round in 10 μmol/L TET group. Compared with 0 μmol/L group, the expression of Ecadherin was increased in 2.5, 5 and 10 μmol/L TET groups， while N-cadherin and Vimentin expression were decreased, and the phosphorylation of AKT and NFκB (p65), the key molecules in PI3K/AKT/NF-κB signaling pathway was inhibited(all P<0.05). Conclusion: TET may reverse the epithelial-mesenchymal transformation of HCT116 cells by inhibiting PI3K/Akt/NFκB signal pathway, and then reduce the ability of cell proliferation and migration and induce apoptosis.

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	Articles by authors
	WANG Yue-xia1
	ZHANG Zhi-jin2
	ZHANG Yu-hao2
	FU Jun2
	QIN Xian-ju2

Cite this article:

WANG Yue-xia1,ZHANG Zhi-jin2,ZHANG Yu-hao2等.

#br# Effect of tetrandrine on human colorectal cancer HCT116 cell line and its potential mechanism

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 336-341.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/336

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