基于生物信息学分析哮喘患者鼻黏膜上皮细胞DNA及α肌动蛋白2甲基化差异性表达

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Abstract Objective: To investigate the relationship between asthma and the differences of DNA methylation in nasal epithelium cells and to detect the methylation level of actin alpha 2(ACTA2) in asthma patients. Methods: Nasal epithelium cell samples from 6 asthma patients and 5 healthy adults were collected for the detection of methylation levels of whole genome by Illumina Infinium MethylationEPIC BeadChip. ACTA2 gene was screened out after the analysis of Gene Ontology function. Then, samples which were collected from 12 asthma patients and 12 healthy adults were detected by bisulfite genomic sequencing PCR to analyse the methylation level of ACTA2 promoter. Results: Illumina BeadChip illustrated that methylation changes were detected in all euchromosomes, most of which occured in gene body or intergenic region, only 10%-20% found in promoter. Meanwhile, hypermethylation and hypomethylation changes were found in the same chromosome; methylation level of ACTA2 promoter was higher in asthma patients than healthy adults(P=0.001). Conclusion: Asthma patients showed genomewide methylation level in nasal epithelium cells; and higher methylation level of ACTA2 promoter.

Received: 06 February 2020

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	ZHANG Li-sha
	QIAN Fen-hong

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ZHANG Li-sha,QIAN Fen-hong.

Bioinformatics analysis of differential methylation of DNA and actin alpha 2 in nasal epithelium cells of asthma patients

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 342-346.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/342

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