人脐带间充质干细胞源胞外囊泡促进施万细胞增殖和迁移

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Abstract Objective: To investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation and migration of schwann cells. Methods: EVs was isolated and purified from supernatant of hUCMSCs by ultrahigh speed centrifugation, morphological characteristics of EVs were observed by transmission electron microscope. Western blotting was used to detect the expression of EVs marker proteins CD63 and CD9. The effects of different concentrations of hUCMSCEVs on proliferation and migration of schwann cells were tested by CCK8 and Transwell chamber experiments. Immunofluorescence assay was used to observe the fusion of EVs and primary schwann cells cell membranes. Results: hUCMSCEVs was round or oval and expressed specific molecular markers CD63 and CD9; Compared with the control group, hUCMSCEVs significantly promoted schwann cells proliferation (P<0.05) and migration (P<0.05). hUCMSCEVs fuse with cell membrane after incubation with schwann cells. Conclusion: hUCMSCEVs can significantly promote proliferation and migration of schwann cells in a concentrationdependent manner.

Received: 15 January 2020

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	Articles by authors
	ZHANG Huan-yan1
	2
	MA Yong-bin2
	DONG Li-yang1
	ZHANG Wen-zhe1
	XUE fei1
	SHAN Wen-qi1
	WANG Xue-feng1

Cite this article:

ZHANG Huan-yan1,2,MA Yong-bin2等.

Human umbilical cord mesenchymal stem cells-derived extracellular vesicles promote proliferation and migration of schwann cells

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 307-311.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/307

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