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Journal of Jiangsu University(Medicine Edition)

	2014 Vol.24 Issue.3 Published 2014-06-30

Article

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		2014 Vol. 24 (3): 0- [Abstract] ( 579 ) [HTML 1KB] [ PDF 314KB] ( 1157 )

	185	Advanced glycation end products induce oxidative stress and mtochondrial dysfunction in human aortic endothelial cells
		DING Yin-Hui-1, Sun-Jing-1, Qian-Yuan-Xia-1, Li-Yun-Zi-1, Gao-Jing-1, Chen-Bin-2
		Objective: To investigate whether advanced glycation end products (AGEs) can induce cell injury and mitochondrial dysfunction in human aortic endothelial cells (HAECs). Methods: HAECs were treated with increasing concentrations (50，100 μg/mL) of AGE-bovine serum albumin (AGE-BSA) for 48 h. The proliferative inhibition of HAECs was measured by CCK-8 method. Contents of ATP and activity of superoxide dismutase (SOD) were determined by the luciferase assay and SOD kits. Reactive oxygen species (ROS) were determined by DCFH-DA staining. Mitochondrial membrane potential (MMP) was observed with JC-1 staining. Oxygen utilization was measured polarographically with a Clark oxygen electrode. Results: Compared with control group，AGE-BSA (50, 100 μg/mL) significantly reduced HAECs proliferation. AGE-BSA significantly increased the levels of ROS, while decreased the activity of SOD compared to the control group. Importantly，AGE-BSAmediated oxidative stress was followed by a collapse of mitochondrial membrane potential (MMP)，the inhibition of ATP generation，and the downregulation of oxygen utilization. Conclusion: AGEBSA induced oxidative stress in HAECs. Moreover，AGE-BSAinduced HAECs injury was related to mitochondrial dysfunction.
		2014 Vol. 24 (3): 185- [Abstract] ( 1397 ) [HTML 1KB] [ PDF 3895KB] ( 1504 )

	190	Effect of cryopreservation on biological characteristics of human adipose-derived mesenchymal stem cells
		LI Xing-Tian, Li-Xiao-Zhan, Ma-Hong, Xu-Hui, Li-Shu, Li-Yu-Mei
		Objective: To provide the experimental support for establishing a lowtemperature stem cell bank for future use, freezethawed human adiposederived mesenchymal stem cells(hADSCs) were compared with fresh hADSCs in vitro. Methods： The hADSCs were isolated from abdomen epiploica adipose tissue by collagenase digestion and subcultured in vitro. The third passage of hADSCs was cryopreserved in liquid nitrogen for 1,3,and 6 months. Cell viability, proliferation potential, expression of cellular markers and multipotential differentiation were studied after thawing using trypan blue staining, MTT method, flow cytometry analysis,Oil Red O staining, von Kossa,and RT-PCR. Results： After thawing, cell survival rate was found to descend as the time of cryopreservation prolonged. MTT revealed high capability for the proliferation. Phenotypic studies revealed that hADSCs were positive for CD29, CD44, CD73, CD90, CD105, CD166 and negative for CD45,and HLA-DR.Functional analysis demonstrated these cells could be differentiated into adipocytes and osteoblasts in lineagespecific medium.RT-PCR results proved that adipogenic and osteogenic related genes could be expressed. No significant difference(P>0.05) was noted before and after the cryopreservation in cell viability, proliferation potential, expression of cellular markers and multipotential differentiation. Conclusions： The hADSCs could maintain acceptable viability, biological characteristics, and multipotential differentiation after cryopreservation.
		2014 Vol. 24 (3): 190- [Abstract] ( 1122 ) [HTML 1KB] [ PDF 4623KB] ( 1921 )

	195	Silencing of FANCF gene by siRNA interference sensitizes lung cancer CALU1 cells to cisplatin
		CHEN Yu-Jiao-1, Li-Jian-1, Jiang-He-Guo-1, Chen-Yong-Chang-2
		Objective: To examine whether silencing of FANCF gene by siRNA interference technique could sensitize lung adenocarcinoma CALU-1 cell line to cisplatin（DDP）. Methods: Three siRNAs targeted to FANCF (FANCF-siRNAs) were designed and synthesized. After separately transfected into CALU-1 cells, the RT-PCR was used to determine the expression of FANCF mRNA, the siRNA with the highest transfection efficiency was selected. Western Blotting was carried out to detect the expression of FANCF protein, and the levels of FANCD2 protein monoubiquitination, which was defined by the radio of monoubiquitinationFANCD2 (L) and nonmonoubiquitinationFANCD2 (S), in CALU-1 cells treated with cisplatin before and after FANCF-siRNA transfection. Immunofluorescence assay was performed to determine the formation of FANCD2 nuclear foci. CCK-8 technique was used to measure the cell proliferation rate of CALU-1 cells treated with DDP pre- and post-transfection of FANCFsiRNA. Results: After transfection of FANCF-siRNA, we found that silencing of FANCF gene decreased the levels of FANCD2 monoubiquitination (L/S ratio), and nuclear foci formation of FANCD2 in CALU-1 cells. Meanwhile, silencing of FANCF gene significantly decreased the cell proliferation rate in CALU-1 cells treated with DDP ( both P< 0.05). Conclusion: Silencing of FANCF gene by FANCF-siRNA transfection could potentiate the sensitivity to cisplatin in CALU-1 cells, suggesting that FANCF gene may be a potential target in therapeutic strategies for the treatment of lung cancer. ［Key words］FANCF; FA/BRCA pathway; siRNA; cisplatin; sensitivity
		2014 Vol. 24 (3): 195- [Abstract] ( 953 ) [HTML 1KB] [ PDF 4672KB] ( 1750 )

	201	Silence of FANCD2 gene of FA/BRCA pathway reverse the resistance to cisplatin in lung cancer A549/DDP cell line
		JIANG He-Guo-1, Dai-Chun-Hua-1, Li-Jian-1, Chen-Yong-Chang-2, Lan Ting-2, Wu-Min-2
		Objective: To investigate the effect of FANCD2 gene silence on the resistance to cisplatin (DDP) in lung adenocarcinoma A549/DDP cell line. Methods: FANCD2 genes of A549/DDP cells were silenced by transfection of the designed and synthesized siRNA targeted FANCD2(FANCD2-siRNA) through Lipofectamine. CCK-8 technique was used to measure the cell proliferation rate of A549 and A549/DDP cells treated with DDP before and after siRNA transfection. Western blotting was carried out to detect the expression of monoubiquitination (L) and non-monoubiquitination (S) FANCD2 protein and L/S ratio before and after siRNA transfection. Immunofluorescence assay was performed to determine the formation of FANCD2 nuclear foci. Results: The cell proliferation rate and the levels of FANCD2 monoubiquitination (L/S ratio) were markedly higher in A549/DDP cells than in A549 cells treated with DDP (P<0.05). After transfection of FANCD2-siRNA, the levels of FANCD2 monoubiquitination, nuclear foci formation of FANCD2, and the cell proliferation rate were significantly decreased in A549 and A549/DDP cells following DDP treatment (P<0.05). Conclusion: Silence of FANCD2 gene inhibited the function of FA/BRCA pathway by decreasing the monoubiquitination level and nuclear focus formation of FANCD2, resulting in the decrease of cell proliferation, and partial reversion of chemoresistance to cisplatin in lung cancer cell of DDP-resistance.
		2014 Vol. 24 (3): 201- [Abstract] ( 1460 ) [HTML 1KB] [ PDF 4674KB] ( 2532 )

	207	Diosgenin affect human gastric cancer BGC-823 and SGC-7901 cells through MAPK pathways
		WU Yuan-Yuan-1, Cui-Guo-Xing-1, Ma-Tie-Liang-2, Ding-Wei-Liang-2, Ge-Zhi-Jun-3 , Tang-Zhi-An-4
		Objective: To discuss the role of diosgenin in the proliferation, invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells via MAPK signaling pathways. Methods: Human gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro and treated with diosgenin. Proliferation rate, cell migration and invasion were measured by MTT method via Transwell assay. And protein expression of apoptosis-associated protein (AAP) BAX, apoptosis inhibitor protein Bcl-2 and Erk1/2,JNK and p38 proteins of MAPK pathways were measured by Western Blot. Results: When BGC-823 and SGC-7901 cells were treated with diosgenin，the proliferation, migration and invasion of BGC-823 and SGC-7901 cells were significantly decreased. The apoptosis of both cells was enhanced to a certain extent. As to the correlation of gastric cancer cells and MAPK pathways，we found that p-p38 protein expression level after diosgenin treated was dramatically down-regulated; however，the expression levels of Erk1/2，JNK，p38，p-Erk1/2 and p-JNK were negligible. Conclusion: Diosgenin might affect the proliferation，invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells through p-p38 of MAPK pathways.
		2014 Vol. 24 (3): 207- [Abstract] ( 1381 ) [HTML 1KB] [ PDF 3234KB] ( 1630 )

	211	*Inhibitive effect of Clinacanthus nutans* (Burm.f.) Lindau n-butanol extracts on Heps hepatoma in mice**
		LIU Xu-1, Guo-Wen-Jie-1, Huang-Dan-Min-1, 2 , Gao-Jing-1
		Objective: To investigate the antitumor activity and life extention effect of Clinacanthus nutans (Burm.f.) Lindau n-butanol extract (CN-N) on tumor loaded mice. Methods:Mice hepatoma carcinoma cells (Heps, grown in donor mice) were transplanted subcutaneously into armpit of the ICR mice to establish the tumor xenografts model. Tumor mice were randomized into four groups: control group, cytoxan group, CN-N low dose group (3 mg·kg-1·d-1), CN-N high dose group (10 mg·kg-1·d-1). Tumor inhibition rate, body weights, immune organs index and life prolonging effect were recorded. Proliferation of tumor cells were detected by western blotting and apoptosis was examined by TUNEL assay. Results: Compared with the control group, CN-N low dose group and high dose group showed remarkable inhibition of tumor weight (t=2.261, 3.140, both P＜0.05);no significant differences on spleen and thymus index of tumor earing mice(both P＞0.05). PCNA expression levels were greatly reduced when treated with low or high concentration of CN-N. Compared with the control group, CN-N high dose group significantly extended the survival time(t=3.416, P=0.003 1). Conclusion: CN-N exhibited good antitumor activity and significantly prolonged the survival time of the tumor-bearing mice.
		2014 Vol. 24 (3): 211- [Abstract] ( 1898 ) [HTML 1KB] [ PDF 4244KB] ( 2276 )

	216	Effect of cardiac fibroblasts on cardiomyocyte hypertrophy induced by high glucose
		ZHAO Long, Liu-Ling-Ling, Li-Xiao-Li, Zhou-Yan-Fang, Wang-Hao, Zhang-Ying-Yu, Kong-Biao, Shen-Dong-Li, Zhang-Guo-Hui
		Objective: To investigate the effect of cardiac fibroblast on cardiomyocyte hypertrophy induced by high glucose. Methods: Neonatal CFs and CMs isolated from 0to 3dayold Sprague-Dawley rats were cultured in vitro,then randomly divided into six groups:CM low glucose group（group A）,CM high glucose group（group B）,CM and CF co-culture high glucose group（group C）,CM-CF co-culture + TGF-β1 neutralizing antibody high glucose group（group D）,CF low glucose group（group E）,CF high glucose group（group F）.After cultured different times (0,6,12,24,48,72),cellular morphologies were observed under the inverted phase contrast microscope, the mRNA levels of cardiomyocyte hypertrophy markers ANP、β-MHC were determinated by RT-PCR, the expression of TGF-β1 evaluated by ELISA. Results: Compared with group A, cell surface area of group B cultured for 48 h increased significantly (P<0.05), group C cultured for 24 h increased significantly (P<0.05) respectively. RT-PCR showed that compared with group A ,the expression of ANP、β-MHC mRNA of group B and D cultured for 12 h increased significantly(P<0.05) ,cultured for 24 h increased at most, group C cultured for 6 h increased significantly (P<0.05),cultured for 12 h increased at most respectively. ELISA showed that compared with group A ,the expression of TGF-β1 of group B cultured for 12 h increased significantly (P<0.05) ,group C cultured for 6 h increased significantly (P<0.05) respectively；compared with group E ,6 h later group F increased significantly (P<0.05) respectively. Conclusion: Cardiac fibroblastsmay via paracrine transforming growth factor-β1 promote cardiomyocyte hypertrophy induced by high glucose.
		2014 Vol. 24 (3): 216- [Abstract] ( 1167 ) [HTML 1KB] [ PDF 3846KB] ( 2120 )

	221	Effect of hepatocyte growth factor on doxorubicininduced H9C2 cells apoptosis
		LIU Ling-Ling, Zhao-Long, Li-Xiao-Li, Zhang-Ying-Yu, Wang-Hao, Zhou-Yan-Fang, Zhang-Guo-Hui
		Objective: To investigate the effect of hepatocyte growth factor(HGF) on doxorubicin(DOX)induced H9C2 cells apoptosis. Methods: The third generation of mesenchymal stem cells(MSCs) were divided into three groups:cultured alone,HGF-siRNA-MSCs and NC-siRNA-MSCs.The concentration of HGF and transforming growth factor β1(TGF-β1) were measured with both ELISA and Western-blot kit.H9C2 cells were exposed to 1.0 μmol/L doxorubicin for 4 hours.Then they were divided into four groups: cultured alone,co-cultured with MSCs,co-cultured with HGF-siRNA-MSCs and co-cultured with NC-siRNA-MSCs.After 24 hours，the apoptosis rate was determined by flow cytometry (FCM). Results: Compared with other groups，the concentration of TGF-β1 in MSCs were significantly increased in the HGF-siRNA-MSCs[(519.23±24.34）pg/mL］ than cultured alone\[（459.65±11.78）pg/mL] and NC-siRNA-MSCs [（459.33±11.78）pg/mL,P<0.05］.The apoptosis rate of H9C2 cells was increased significantly in co-cultured with HGF-siRNA-MSCs（18.54±0.64)% than co-cultured with MSCs(6.65±0.49)% and NC-siRNA-MSCs ［(9.70 ± 1.62)%,P<0.05］. Conclusion: HGF prevented DOX-induced H9C2 cellsapoptosis by inhibition of TGF-β1.
		2014 Vol. 24 (3): 221- [Abstract] ( 1193 ) [HTML 1KB] [ PDF 4540KB] ( 2146 )

	226	Chemotactic effect of human cytomegalovirus to peripheral blood mononuclear cells in vitro
		WEI Wei-1, Gu-Shao-Qing-2, Li-Wen-Jing-1, Ma-Xiao-Meng-1, Zhao-Yuan-1
		Objective: To explore the chemotactic effect of human cytomegalovirus (HCMV) on peripheral blood mononuclear cells (PBMC) in vitro, and the influence of dexamethasone onchemotaxis. Methods: We took different concentrations (5, 10, 20, 40, 60, 80, 100, 1 000 TCID50)of HCMV AD169 to vaccinate human embryonic lung fibroblast cells(HELF), and extracted culture media supernatant, which was used to attract PBMC at 24, 48, 72, 96 h. Then we observed and counted the migratory PBMC under high power lens of the microscope, and compared the strength of chemotaxis. We also added dexamethasone at different concentrations (0.125, 1.25, 12.5, 25, 50,100 μg/mL) to infect HELF as control. Results: There were no difference in chemotaxis among different concentration of HCMV between 5 TCID50 and 10 TCID50, 80 TCID50 and 1 000 TCID50, 1 00 TCID50 and 1 000 TCID50 among 24, 48, 72, 96 h (both P＞0.05). The chemotactic effect of HCMV between 10 TCID50 and 20 TCID50, 20 TCID50 and 40 TCID50 , among 24, 48, 72 h had no statistical difference( both P>0.05). Compared with the control goup( 0 μg/mL), the chemotaxis of culture media supernatant of the dexamethasone at 12.5μg/mL and 12.5 μg/mL had no statistical difference(both P＞0.05), while dexamethasone at 25, 50, 100 μg/mL had statistical difference (both P<0.01). Conclusion: The supernatant of HCMV infected HELF had chemotaxis to PBMC, which was increased with the culture time increasing. Dexamethasone could restrain inhibitory effect on chemotaxis, which was increased with the drug concentration increasing.
		2014 Vol. 24 (3): 226- [Abstract] ( 1137 ) [HTML 1KB] [ PDF 3662KB] ( 1830 )

	230	A pilot study of serum microRNA profiles as a novel biomarker for primary biliary cirrhosis
		PAN Teng-Li-1, 2 , Sun-Li-2, Zhou-Xing-Bei-2, Chen-Li-2, Yu-Xue-Jun-2, Tan-You-Wen-2
		Objective: To screen serum microRNA profiles by high throughput sequencing technology method as diagnostic markers for primary biliary cirrhosis (PBC). Methods: First, we employed Illumina GA IIx deep sequencing for the initial screening to indicate the read numbers of microRNAs expression in serum pool of 3 PBC and 3 healthy controls, respectively. Second, qRT-PCR validation study was conducted in more samples. Finally, computer analysis was conducted to predict target genes and biological functions. Results: Twenty-two miRNAs, whose fold change were greater than 2, were filtered out by high throughput sequencing technology. After qRT-PCR validation, the serum expression levels of miR-122, miR-34a and miR-141 were significantly higher in PBC, consistent with the sequencing results. Target genes of prediction results showed that microRNAs played a role in signal transduction, in the nucleus and cytoplasm, through combined with protein. Conclusion: The distinguishing expressed microRNAs had certain reference value on the diagnosis of PBC.
		2014 Vol. 24 (3): 230- [Abstract] ( 1379 ) [HTML 1KB] [ PDF 3975KB] ( 1668 )

	235	*Construction of a spvC gene-deleted mutant in Salmonella enterica* serovar Typhi**
		CHEN Qiang-1, Wu-Chun-Xue-1, Yu-Xiao-Jun-2, Li-Hong-3, Zhu-Chun-Hui-1, Liu-Xiao-Yan-4
		Objective: To investigate the function of spvC gene, the deletion mutant of the spvC gene was constructed in Salmonella enterica serovar Typhi. Methods: As the genomic information, two pair′s primers were designed, upper-and down-stream of the spvC gene to amplify two homologous DNA fragments. The homologous recombinant DNA fragment of the defective target gene was cloned into the suicide plasmid pCVD442 which was then transferred into the target cell of S.enterica serovar Typhi. The recombination was visualized by PCR, and the complete recombinant strain was selected as the spvC gene deleted mutant strain and confirmed by the corresponding sequencing analysis. Results: A deletion of 711 bp of the spvC gene was confirmed by PCR and sequencing analysis. Conclusion: The spvC gene-deleted mutant of S.enterica serovar Typhi was generated successfully, and it was a foundation to study the detail functions of the spvC gene in S. enterica serovar Typhi.
		2014 Vol. 24 (3): 235- [Abstract] ( 1398 ) [HTML 1KB] [ PDF 3530KB] ( 2154 )

	240	Effect of short-term continuous subcutaneous insulin infusion treatment on newly diagnosed type 2 diabetes related to serum high-sensitive C-reactive protein
		TANG Bing-Qian, Hu-Hao, Qian-Wei-Yun, Zhu-Tian-Yi, Yu-Shu-Qin, Sun-Wen-Jun, Yang-Ling, Wang-Dong, Wang-Ji-Fang, Yuan-Guo-Yue
		Objective: To investigate the changes of short-term continuous subcutaneous insulin infusion (CSII) on serum hs-CRP levels in patients with newlydiagnosed type 2 diabetes mellitus, and to identify the influencing factors for changes of hs-CRP. Methods: A total of 70 normal controls, 120 patients with newly diagnosed type 2 diabetes mellitus were treated with CSII for 2 weeks. Oral glucose tolerance test and insulin releasing test were performed before and after intensive insulin therapy; meanwhile, blood lipid, hs-CRP and other parameters were measured. Results: ①Serum hs-CRP was significantly higher ［2.76(0.81~6.33) mg/L］ in newly diagnosed type 2 diabetes mellitus than normal controls ［0.48(0.18~0.98)mg/L］. ② In the T2DM group，there was a significant decrease in fasting plasma glucose (FPG), 2 h plasma glucose (2hPG), HOMA-IR and hs-CRP (P<0.01 or P<0.05), a significant increase in homeostasis model assessment for insulin function.③The changes of serum CRP was positively correlated with Δ SBP,ΔFPG, ΔFINS, ΔTC,ΔLDL-C, ΔHOMA-β, ΔHOMA-IR (r=0.287,0. 324, 0.236, 0.257,0.366, 0.201, 0.230, P<0.05). The multiple regression analysis showed that ΔFPG(β=0.214，P<0.05), ΔFINS(β=0.376，P<0.01), ΔLDL-C(β=0.263，P<0.05), ΔHOMA-β(β=0.352，P<0.01), ΔHOMA-IR(β=0.279，P<0.05)were independent related factors in the influence of the changes of hs-CRP levels. Conclusion: In type 2 diabetic patients, elevated serum hs-CRP levels were significantly decreased after CSII treatment. The multiple regression analysis showed that ΔFPG, ΔLDL-C, ΔFINS, ΔHOMA-β and ΔHOMA-IR were independent related factors in the influence of the changes of hs-CRP levels.
		2014 Vol. 24 (3): 240- [Abstract] ( 1054 ) [HTML 1KB] [ PDF 3538KB] ( 1893 )

	245	Association between neck circumference, insulin resistance and highly sensitive C-reactive protein in newly diagnosed type 2 diabetes patients with metabolic syndrome
		QIAN Wei-Yun, Yu-Shu-Qin, Zhu-Tian-Yi, Tang-Bing-Qian, Sun-Wen-Jun, Hu-Hao, YE Jing-Jing, Wang-Dong, Wang-Ji-Fang, Yang-Ling, Yuan-Guo-Yue
		Objective: To investigate neck circumferences in patients with type 2 diabetes mellitus (T2DM) with metabolic syndrome(MS), and to study the association between neck circumference, insulin resistance and highly sensitive C-reactive protein. Methods: A total of 165 subjects were enrolled in the study, including 55 healthy subjects and 110 patients with newlydiagnosed type 2 diabetes(T2DM) which were divided into T2DM group and T2DM with MS group. Oral glucose tolerance test(OGTT) and insulin releasing test were performed in all subjects, meanwhile, neck circumference, blood lipid, hs-CRP and other items were measured. Results: Compared with control group and T2DM group, the neck circumference was increased in MS group[(38.47±3.68) vs (35.17±3.09)cm, (38.47±3.68) vs (36.86±2.92)cm, P<0.05 or P<0.01, respectively]. The serum hs-CRP in MS group was higher than in normal control group and T2DM group[2.77(1.19~5.55) vs 0.40(0.18~0.98)mg/L, 2.77(1.19~5.55) vs 1.74(0.81~5.33)mg/L, P<0.01, respectively]. Subjects in the upper neck circumference fertile had higher levels of BMI, WC, WHR, FINS, PSI, TG, HOMAIR and hsCRP, while lower levels of HDLC when compared to subjects in the middle or lower neck circumference fertile(P<0.05 or P<0.01). The neck circumference was positively correlated with BMI, WC, WHR, DBP, FPG, 2hPG, FINS, PSI, TG， LDL-C, HOMAIR and hsCRP, while negatively correlated with HDL-C(P<0.05 or P<0.01). Logistic regression analysis showed that neck circumference, WC and TG were independent risk factors for T2DM with MS, while HDL-C was the protective one(P<0.01). Conclusion: Newlydiagnosed type 2 diabetes mellitus patients with MS tend to have bigger neck circumference, the neck circumference was associated with insulin resistance and hs-CRP. The neck circumference was the risk factor for T2DM with MS.
		2014 Vol. 24 (3): 245- [Abstract] ( 856 ) [HTML 1KB] [ PDF 4066KB] ( 1568 )

	250	Expression of NDRG-1，COX-2 and VEGF in cervical squamous cell carcinoma tissue and its clinical significance
		YUAN Dong-Lan-1, Qian-Hua-1*, Wang-Hua-1, Jiang-Jia-Bao-2, Zhu-Xiao-Wei-3, Jiao-Xia-3, Jiang-Xiao-Qin-3, Xiao-Wei-3, Yu-Hong-3
		Objective: To observe the expression of N-myc downstream regulated gene-1(NDRG-1), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in cervical squamous cell carcinoma tissue and to elucidate the mechanism of cervical squamous cell carcinoma invasion and metastasis. Methods: Expressions of NDRG-1, COX-2 and VEGF in tissue samples from 80 cervical squamous cell carcinoma cases and 15 healthy control cases were detected by immunohistochemistry; and their correlation with characteristics of clinical materials and prognosis in cervical squamous cell carcinoma were evaluated. Results: Compared with the normal cervical tissue, the positive expression rate of NDRG-1 in cervical squamous cell carcinoma tissue was significantly lower(P<0.01), while the positive rate of COX-2， VEGF was higher(both P<0.01).There was no significant difference between positive rate of NDRG-1,COX-2， VEGF expressions and clinicopathologic data of cervical squamous cell carcinoma patients including age and tumor size(P>0.05). Positive expression rate of NDRG-1, COX-2 and VEGF were significant correlated with tumor histopathological grade,clinical FIGO stages and lymph node metastasis(P<0.01).There was statistically significant positive correlation among NDRG-1,COX-2 and VEGF expressions in cervical squamous cell carcinoma tissue(P<0.01). There was statistically correlation between positive rate of NDRG-1,COX-2 and VEGF expression with survival time by KaplanMeier analysis. Conclusion: The abnormal expressions of NDRG-1,COX-2 ,VEGF might participate in the invasion and metastasis of cervical squamous cell carcinoma. The joint detection of NDRG-1, COX-2, VEGF might provide some guiding significance to judge the prognosis of cervical squamous cell carcinoma.
		2014 Vol. 24 (3): 250- [Abstract] ( 1246 ) [HTML 1KB] [ PDF 4728KB] ( 2048 )

	255	Effects of polyunsaturated fatty acids on the recurrence of atrial fibrillation: a metaanalysis
		LI Xiao-Li, Zhang-Bao-Wei, Liu-Ling-Ling, Zhao-Long, Shen-Dong-Li, Kong-Biao, Zhang-Guo-Hui
		Objective: To evaluate the efficacy of polyunsaturated fatty acids (PUFA) on the recurrence of atrial fibrillation. Methods: The databases of Medline, EMBASE, Elsevier and Cochrane library were searched for randomized controlled trials. Quality assessment and data extraction were performed by two independent reviewers. Statistical analyses were conducted with RevMan 5.0 and Stata 10.0 software. Results: Studies with 1 839 patients were enrolled into this metaanalysis. Meta-analysis result showed that treatment with PUFA did not reduce the rate of recurrence of atrial fibrillation (RR=0.96, 95% CI 0.75-1.22, P=0.74). Sub-group analysis showed that PUFA could reduce the rate of recurrence of atrial fibrillation in the sub-group that patients were treated with PUFA more than 28 days before the following-up (RR=0.720,95% CI 0.622-0.835, P<0.01). Conclusion: The results of the present meta-analysis of randomized controlled trials suggested that treatment with PUFA did not reduce the rate of recurrence of atrial fibrillation.
		2014 Vol. 24 (3): 255- [Abstract] ( 777 ) [HTML 1KB] [ PDF 3275KB] ( 1678 )

	260	Expression and clinical significance of long non-coding RNA HOTAIR in patients with esophageal squamous cell carcinoma
		ZHANG Zhao-Yue-1, Yin-Yue-1, Mou-Xiao-1, Wu-Shi-1, Mao-Chao-Ming-2, Chen-De-Yu-3
		Objective: To explore the expression and clinical significance of long non-coding RNA HOTAIR (lncRNA HOTAIR) in esophageal squamous cell carcinoma (ESCC) and analyze its correlation with transforming growth factor β1（TGF-β1）. Methods: Quantitative real-time polymerase chain reaction was used to detect the expressions of HOTAIR mRNA and TGF-β1 mRNA in 57 ESCC tissues and matched tumor-free tissues. The relationship between HOTAIR mRNA and clinico-pathological features of ESCC was also analyzed. Results: In comparison to matched tumor-free tissues, the expressions of HOTAIR mRNA (Z=-2.507，P=0.012) and TGF-β1 mRNA (Z=-2.038，P=0.042) were found to be elevated and lowered, respectively, in ESCC tissues. Up-regulation of HOTAIR expression was correlated with tumor differentiation degree and clinical stage. The expression of HOTAIR was negative correlated with TGF-β1 (r=-0.406，P=0.002). Conclusion: lncRNA HOTAIR in ESCC tissues may play a significant role in the occurrence and development of ESCC through TGF-β1 signaling pathways.
		2014 Vol. 24 (3): 260- [Abstract] ( 1599 ) [HTML 1KB] [ PDF 3799KB] ( 1559 )

	264
		ZHAO Zi-Yu, Xiang-Jing-Ying, Zhang-Yun

		2014 Vol. 24 (3): 264- [Abstract] ( 796 ) [HTML 1KB] [ PDF 2081KB] ( 1705 )

	267
		MENG Fan-Wen-1, Wu-Xiao-Liang-1, Liu-Mei-Xia-2, Wang-Li-1, Wang-Jia-Wei-1, Chen-Zu-Xian-1

		2014 Vol. 24 (3): 267- [Abstract] ( 700 ) [HTML 1KB] [ PDF 2201KB] ( 1778 )

	270
		MA Jing, Ye-Wei, Guo-Jian-Hua, Lu-Jun, Yuan-Guo-Qing

		2014 Vol. 24 (3): 270- [Abstract] ( 886 ) [HTML 1KB] [ PDF 2079KB] ( 1744 )

	272
		ZHAO Jing-1, Qian-Jin-Jun-1, Gong-Qi-Xia-2

		2014 Vol. 24 (3): 272- [Abstract] ( 1250 ) [HTML 1KB] [ PDF 2099KB] ( 1555 )

	275


		2014 Vol. 24 (3): 275- [Abstract] ( 572 ) [HTML 1KB] [ PDF 1101KB] ( 1660 )

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