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| Diosgenin affect human gastric cancer BGC-823 and SGC-7901 cells through MAPK pathways |
| WU Yuan-yuan1， CUI Guo-xing1， MA Tie-liang2， DING Wei-liang2， GE Zhi-jun3， TANG Zhi-an4 |
| （1.Department of Gastroenterology,2.Central Laboratory，3.Department of Critical Care Medicine，4.Department of Traditional Chinese Medicine，the Affiliated Yixing Hospital of Jiangsu University，Yixing Jiangsu 214200，China） |
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Abstract Objective: To discuss the role of diosgenin in the proliferation, invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells via MAPK signaling pathways. Methods: Human gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro and treated with diosgenin. Proliferation rate, cell migration and invasion were measured by MTT method via Transwell assay. And protein expression of apoptosis-associated protein (AAP) BAX, apoptosis inhibitor protein Bcl-2 and Erk1/2,JNK and p38 proteins of MAPK pathways were measured by Western Blot. Results: When BGC-823 and SGC-7901 cells were treated with diosgenin，the proliferation, migration and invasion of BGC-823 and SGC-7901 cells were significantly decreased. The apoptosis of both cells was enhanced to a certain extent. As to the correlation of gastric cancer cells and MAPK pathways，we found that p-p38 protein expression level after diosgenin treated was dramatically down-regulated; however，the expression levels of Erk1/2，JNK，p38，p-Erk1/2 and p-JNK were negligible. Conclusion: Diosgenin might affect the proliferation，invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells through p-p38 of MAPK pathways.
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| [1] |
. [J]. Journal of Jiangsu University(Medicine Edition), 2018, 28(05): 454-458. |
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