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| Construction of a spvC gene-deleted mutant in Salmonella enterica serovar Typhi |
| CHEN Qiang1， WU Chunxue1， YU Xiaojun2， LI Hong3， ZHU Chunhui1， LIU Xiaoyan4 |
| (1.Department of Internal Medicine,Jiangxi Children′s Hospital, Nanchang Jiangxi 330006;2.Department of Clinical Laboratory,Jiangxi Children′s Hospital,Nanchang Jiangxi 330006;3.Department of Laboratory Center，Jiangxi Children′s Hospital,Nanchang Jiangxi 330006;4.Medical Board of Nanchang University，Nanchang Jiangxi 330006,China） |
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Abstract Objective: To investigate the function of spvC gene, the deletion mutant of the spvC gene was constructed in Salmonella enterica serovar Typhi. Methods: As the genomic information, two pair′s primers were designed, upper-and down-stream of the spvC gene to amplify two homologous DNA fragments. The homologous recombinant DNA fragment of the defective target gene was cloned into the suicide plasmid pCVD442 which was then transferred into the target cell of S.enterica serovar Typhi. The recombination was visualized by PCR, and the complete recombinant strain was selected as the spvC gene deleted mutant strain and confirmed by the corresponding sequencing analysis. Results: A deletion of 711 bp of the spvC gene was confirmed by PCR and sequencing analysis. Conclusion: The spvC gene-deleted mutant of S.enterica serovar Typhi was generated successfully, and it was a foundation to study the detail functions of the spvC gene in S. enterica serovar Typhi.
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2014, Vol. 24 
