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| Chemotactic effect of human cytomegalovirus to peripheral blood mononuclear cells in vitro |
| WEI Wei1, GU Shao-qing2, LI Wen-jing1, MA Xiao-meng1, ZHAO Yuan1 |
| （1.School of Clinical Medicine, Jiangsu University, Zhenjiang Jiangsu 212001; 2.Department of
Pediatrics, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002,China) |
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Abstract Objective: To explore the chemotactic effect of human cytomegalovirus (HCMV) on peripheral blood mononuclear cells (PBMC) in vitro, and the influence of dexamethasone onchemotaxis. Methods: We took different concentrations (5, 10, 20, 40, 60, 80, 100, 1 000 TCID50)of HCMV AD169 to vaccinate human embryonic lung fibroblast cells(HELF), and extracted culture media supernatant, which was used to attract PBMC at 24, 48, 72, 96 h. Then we observed and counted the migratory PBMC under high power lens of the microscope, and compared the strength of chemotaxis. We also added dexamethasone at different concentrations (0.125, 1.25, 12.5, 25, 50,100 μg/mL) to infect HELF as control. Results: There were no difference in chemotaxis among different concentration of HCMV between 5 TCID50 and 10 TCID50, 80 TCID50 and 1 000 TCID50, 1 00 TCID50 and 1 000 TCID50 among 24, 48, 72, 96 h (both P＞0.05). The chemotactic effect of HCMV between 10 TCID50 and 20 TCID50, 20 TCID50 and 40 TCID50 , among 24, 48, 72 h had no statistical difference( both P>0.05). Compared with the control goup( 0 μg/mL), the chemotaxis of culture media supernatant of the dexamethasone at 12.5μg/mL and 12.5 μg/mL had no statistical difference(both P＞0.05), while dexamethasone at 25, 50, 100 μg/mL had statistical difference (both P<0.01). Conclusion: The supernatant of HCMV infected HELF had chemotaxis to PBMC, which was increased with the culture time increasing. Dexamethasone could restrain inhibitory effect on chemotaxis, which was increased with the drug concentration increasing.
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2014, Vol. 24 
