Alterated expression and role of calcium sensing receptor and calpain induced by anoxia/reoxygenation in rat spinal cord neurons
JIANG Run-yu1, SUN Ji-fu1, HE Wei1, ZHANG Zhi-jian2, HUANG Yong-hui1
(1. Department of Orthopaedics, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Basic and Clinic Medical Research Center, School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To investigate the alterated expression and role of calcium sensing receptor(CaSR) and calpain in apoptosis of rat spinal cord neurons induced by anoxia/reoxygenation (A/R). Methods: After spinal anoxia/reoxygenation model was established by culturing primary spinal cell of neonatal rat in vitro, the spinal cells were divided into four groups randomly：the control group，A/R group，calcimimetic group(GdCl3 group) and inhibitor group (NPS2390)．The apoptotic rate of spinal cells was determined by Hoechst 33258 assay，the expression of CaSR and calpain was detected by fluorescence immunoassay and Western blotting. Results: In A/R group，there was a significant increase in the apoptotic rate and the levels of CaSR, calpain and Bax compared to the control group(P<0.01)．Howerer，the above markers increased more in GdCl3 group，and these parameters decreased in inhibitor group. Conclusion: During the process of hypoxia/reoxygenation injury in rat spinal cord neurons, the expressions CaSR and calpain increased, accompanied with increasing of the apoptosis rate.
2016, Vol. 26 
