Abstract:[Abstract]Objective: To investigate the effect of cyclophilin A(CyPA) on ox-LDL-induced activation and apoptosis in RAW264.7 cells. Methods: RAW264.7 cells were incubated with 40 mg/L ox-LDL for 24 h, Western blotting analysis was employed to detect the CyPA expression. After transfected with CyPA siRNA, the cells were incubated with 40 mg/L ox-LDL for 24 h. The positive rate of CD80, CD86 and the apoptosis rate were determinated by flow cytometry analysis. Results: After incubating with ox-LDL, the level of CyPA was upregulated. The percentage of CD80 positive cells,CD86 positive cells and apoptotic cells were significantly decreased in CyPA knockdown cells compared with the negative control siRNA cells after the intervention of oxLDL. Conclusion: CyPA may mediate the oxLDLinduced activation and apoptosis in RAW264.7 cells.
薛志强,李静,张薪茹,周红,严金川,王中群,袁伟. 亲环蛋白A对氧化低密度脂蛋白诱导RAW264.7细胞活化与凋亡的影响[J]. 江苏大学学报:医学版, 2016, 26(02): 128-131.
XUE Zhi-qiang, LI Jing, ZHANG Xin-ru, ZHOU Hong, YAN Jin-chuan, WANG Zhong-qun, YUAN Wei. Effect of CyPA on ox-LDL-induced activation and apoptosis in RAW264.7 cells. Journal of Jiangsu University(Medicine Edition), 2016, 26(02): 128-131.
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