The regulation of TACSTD2 in the chemosensitivity of human esophageal squamous cell carcinoma
WU Dan1, ZHOU Ling2, MI Lei1, ZHOU Yue-peng1, CHEN De-yu1
(1. Department of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Intensive Care Unit, Changzhou Wujin People′s Hospital, Changzhou Jiangsu 213017, China)
Abstract：Objective: To investigate the effects of tumorassociated calcium signal transducer 2 (TACSTD2) on cisplatin of esophageal squamous cell carcinoma (ESCC) and the underlying mechanism. Methods: ESCC tissues and adjacent paracarcinoma tissues were collected for chip and IHC analysis. Choosing ESCC cells ECA 109, KYSE 30 and cisplatin(DDP) resistant cells ECA 109/DDP and KYSE 30/DDP as object treated with TACSTD2siRNA, negative controlsiRNA and nontreatment, the expression of TACSTD2 were detected by RTPCR and Western blotting. CCK8 assay and flow cytometry were used to detect the DDP IC50 and the apoptosis of these cells. The expression of TACSTD2, multidrug resistance protein 1(MDR1), mitogenactivated protein kinase(MAPK) and pMAPK proteins were detected by Western blotting. Results: The expression of TACSTD2 in ESCC tumors were higher than those of adjacent paracarcinoma tissues. After transfection with siRNATACSTD2, the relative expression of TACSTD2 and IC50 decreased more significantly than those of negative control group (P<0.01). With the treatment of cisplatin, TACSTD2knockdown cells showed significantly higher apoptotic rate. The protein expression of pMAPK and MDR1 in TACSTD2 transfected groups reduced significantly. Conclusion: Knockdown of TACSTD2 could make ESCC cells more sensitive to cisplatin, which is related to the downregulation of MAPK/MDR1 signaling pathway.
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2019, Vol. 29 
