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| Analysis of correlation between EGR3 or VEGF protein and different degrees of coronary stenosis in plateau area |
| SHI Mengzhen1,2, BAI Yuting2, ZHOU Baili2, SU Xiaoling2, LIU Yanmin2, GUO Wenlu3, WEI Xiaojuan2, WUGENG Suya2 |
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Abstract Objective To investigate the correlation between different degrees of coronary stenosis and protein factors such as human early growth response 3 (EGR3) and vascular endothelial growth factor (VEGF), and to screen out the protein factors with the strongest correlation with EGR3 and VEGF. To explore the potential new biomarkers for rapid diagnosis of coronary heart disease (CHD) and assessment of coronary stenosis degree in plateau area. MethodsA total of 172 patients hospitalized in the Department of Cardiology, Qinghai Provincial Peoples Hospital (2 260 m above sea level) were selected, and divided into case group (n=126, including 38 cases in the atherosclerosis group, 71 cases in the moderate stenosis group and 17 cases in the severe stenosis group) and control group (n=46) according to the degree of coronary stenosis. Protein factors such as EGR3 and VEGF were detected, and statistically analyzed. By using Kyoto Encyclopedia of Genes and Genomes (KECG) analysis, the potential new biomarkers for rapid diagnosis of CHD and evaluation of coronary stenosis degree in plateau area were explored. ResultsThe indexes of human monocyte/megakaryocyte major histocompatibility complex receptor 1 (MMR1), EGR3, human thromboxane B2 (TXB2), human serum response factor (SRF), human platelet activating factor (PAF), VEGF, human cyclicAMP response element binding protein (CREB), human thrombopoietin (TPO), thrombospondin 1 (THBS1) all significantly varied from the atherosclerosis group, moderate stenosis group or severe stenosis group to the control group (all P<005). Spearman correlation analysis showed that serum VEGF was positively correlated with TXB2, SRF, PAF, EGR3, CREB, TPO and THBS1 (r=0.460, 0.644, 0.539, 0.462, 0.740, 0.702, 0.673, all P<0.05). Among them, the correlation between serum CREB and VEGF was the strongest. Serum EGR3 was positively correlated with MMR1, TXB2, SRF, PAF, VEGF, CREB, TPO and THBS1 (r=0.405, 0.682, 0.780, 0.700, 0.462, 0.686, 0.551, 0.245, all P<0.05). Among them, the correlation between serum SRF and EGR3 was the strongest. KEGG analysis showed that SRF regulated proliferation and differentiation in the downstream of VEGF in the pathway hsa04010. In the pathway hsa04926, CREB regulated angiogenesis in the upstream of VEGF. In the pathway hsa04066, hypoxia inducible factor regulated angiogenesis in the upstream of VEGF. ConclusionThe factors of MMR1, EGR3, TXB2, SRF, PAF, VEGF, CREB, TPO and THBS1 are correlated with the severity of coronary stenosis. VEGF, CREB, SRF and EGR3 may be come new biomarkers for the diagnosis of CHD in plateau area.
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