Abstract:Objective: To detect the expression of eosinophil chemotactic protein 2 (eotaxin-2) in the serum of patients with colorectal cancer (CRC), and investigate its clinical significance. Methods: A total of 50 CRC patients seeking medical care in the Gastroenterology Department of Affiliated Hospital of Jiangsu University from January 2020 to February 2021 were selected as the CRC group, and 40 healthy subjects in the same period were selected as the healthy controls. The serum eotaxin-2 levels were detected by enzyme-linked immunosorbent assay (ELISA). The association between eotaxin-2 level and the clinicopathological features of CRC was analyzed, and the value of serum eotaxin-2 in the diagnosis of CRC was evaluated by the receiver operating characteristic (ROC) curve. Results: The expression level of serum eotaxin-2 was significantly higher in CRC than that in the healthy controls (Z=-3.93, P<0.01). No significant differences were found among the expression levels of serum eotaxin-2 with respect to the gender, age, lesion location and tumor size (all P>0.05), but was significantly correlated with the lymph-node metastasis, distant metastasis and TNM stage (all P<0.01). The area under the ROC was 0.742 (95% CI: 0.639-0.845, P<0.01). The sensitivity and specificity of serum eotaxin-2 for the diagnosis of CRC were 68.0% and 77.5%, respectively. Conclusion: Serum eotaxin2 level is increased in CRC patients, and may be a candidate biomarker for the CRC diagnosis.
[1]Siegel RL, Miller KD, Fuchs HE, et al. Cancer statistics, 2021[J]. CA Cancer J Clin, 2021, 71(1): 7-33.
[2]Worm rntoft MB. Review of bloodbased colorectal cancer screening: How far are circulating cellfree DNA methylation markers from clinical implementation?[J]. Clin Colorectal Cancer, 2018, 17(2): e415-e433.
[3]Chang W, Wu L, Cao F, et al. Development of autoantibody signatures as biomarkers for early detection of colorectal carcinoma[J]. Clin Cancer Res, 2011, 17(17): 5715-5724.
[4]Grothey A, Venook AP. Optimizing adjuvant therapy for localized colon cancer and treatment selection in advanced colorectal cancer[J]. J Natl Compr Canc Netw, 2018, 16(5S): 611-615.
[5]Conlon KC, Miljkovic MD, Waldmann TA. Cytokines in the treatment of cancer[J]. J Interferon Cytokine Res, 2019, 39(1): 6-21.
[7]Korbecki J, Kojder K, Simińska D, et al. CC chemokines in a tumor: A review of procancer and anticancer properties of the ligands of receptors CCR1, CCR2, CCR3, and CCR4[J]. Int J Mol Sci, 2020, 21(21): 8412.
[8]Ahmadi Z, Hassanshahi G, Khorramdelazad H, et al. An overlook to the characteristics and roles played by eotaxin network in the pathophysiology of food allergies: allergic asthma and atopic dermatitis[J]. Inflammation, 2016, 39(3): 1253-1267.
[9]Ablin JN, EntinMeer M, Aloush V, et al. Protective effect of eotaxin2 inhibition in adjuvantinduced arthritis[J]. Clin Exp Immunol, 2010, 161(2): 276-283.
[10]Coleman JM, Naik C, Holguin F, et al. Epithelial eotaxin2 and eotaxin3 expression: relation to asthma severity, luminal eosinophilia and age at onset[J]. Thorax, 2012, 67(12): 1061-1066.
[11]Zajkowska M, Mroczko B. Eotaxins and their receptor in colorectal cancer —A literature review[J]. Cancers (Basel), 2020, 12(6): 1383.
[12]Cheadle EJ, Riyad K, Subar D, et al. Eotaxin2 and colorectal cancer: a potential target for immune therapy[J]. Clin Cancer Res, 2007, 13(19): 5719-5728.
[13]Cho H, Lim SJ, Won KY, et al. Eosinophils in colorectal neoplasms associated with expression of CCL11 and CCL24[J]. J Pathol Transl Med, 2016, 50(1): 45-51.
[14]Tang Z, Kang B, Li C, et al. GEPIA2: an enhanced web server for largescale expression profiling and interactive analysis[J]. Nucleic Acids Res, 2019, 47(W1): W556-W560.
[15]Olsen RS, Nijm J, Andersson RE, et al. Circulating inflammatory factors associated with worse longterm prognosis in colorectal cancer[J]. World J Gastroenterol, 2017, 23(34): 6212-6219.
[16]Gong D, Li Z, Ding R, et al. Extensive serum biomarker analysis in patients with nasopharyngeal carcinoma[J]. Cytokine, 2019, 118: 107-114.
[17]Wang C, Wang Y, Hong T, et al. Blocking the autocrine regulatory loop of Gankyrin/STAT3/CCL24/CCR3 impairs the progression and pazopanib resistance of clear cell renal cell carcinoma[J]. Cell Death Dis, 2020, 11(2): 117.