Expression analysis of lysophosphatidylcholine acyltransferase 1 gene in patients with acute myeloid leukemia
CHEN Qin1, XU Zijun1, LIN Jiang1, QIAN Jun2, QIAN Wei3
(1. Laboratory Center, 2. Department of Haematology, 3. Department of OtolaryngologyHead and Neck Surgery, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)
Abstract:Objective:To explore the expression status of lysophosphatidylcholine acyltransferase 1 (LPCAT1) gene and its clinical significance in patients with acute myeloid leukemia (AML). Methods:Quantitative realtime PCR was used to examine the LPCAT1 mRNA expression in bone marrow samples from 60 primary AML patients and 27 healthy donors. Results: LPCAT1 mRNA expression was significant lower in AML than controls(P<0.001). LPCAT1 mRNA lowexpression was found in 27 (45%) of 60 AML cases but none in the 27 controls, the difference was statistically significant (χ2=17.618,P<0.001).There was no correlation between the lowexpression of LPCAT1 mRNA with the age, sex, white blood cell count, platelet count, hemoglobin concentration, FAB subtypes and AML karyotypic groups (P>0.05). The receiver operating characteristic (ROC) curve revealed LPCAT1 mRNA expression might serve as a potential diagnostic marker for differentiating AML from controls with an area under the ROC curve of 0.896 (95% CI:0.825~0.967, P<0.001). At the cutoff value of 0.173 of LPCAT1 mRNA expression, the sensitivity and the specificity were 88.3% and 81.5%, respectively.Conclusion: LPCAT1 lowexpression is a frequent molecular event in AML, and it may be used to assist the diagnosis of AML.
[1]Santos CR, Schulze A. Lipid metabolism in cancer[J]. FEBS J, 2012, 279 (15): 2610-2623.
[2]Grupp K, Sanader S, Sirma H, et al. High lysophosphatidylcholine acyltransferase 1 expression independently predicts high risk for biochemical recurrence in prostate cancers[J]. Mol Oncol, 2013, 7(6): 1001-1011.
[3]Zhou X, Lawrence TJ, He Z, et al. The expression level of lysophosphatidylcholine acyltransferase 1 (LPCAT1) correlates to the progression of prostate cancer[J]. Exp Mol Pathol, 2012, 92(1): 105-110.
[4]ShidaSakazume T, EndoSakamoto Y, Unozawa M, et al. Lysophosphatidylcholine acyltransferase 1 overexpression promotes oral squamous cell carcinoma progression via enhanced biosynthesis of plateletactivating factor[J]. PLoS One, 2015, 10(3): e0120143.
[5]Morita Y, Sakaguchi T, Ikegami K, et al. Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression[J]. J Hepatol, 2013, 59(2): 292-299.
[6]Lebok P, von Hassel A, Meiners J, et al. Upregulation of lysophosphatidylcholine acyltransferase 1 (LPCAT1) is linked to poor prognosis in breast cancer[J]. Aging (Albany NY), 2019, 11(18): 7796-7804.
[7]Abdelzaher E, Mostafa MF. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) upregulation in breast carcinoma contributes to tumor progression and predicts early tumor recurrence[J]. Tumour Biol, 2015, 36(7): 5473-5483.
[8]Wei C, Dong X, Lu H, et al. LPCAT1 promotes brain metastasis of lung adenocarcinoma by upregulating PI3K/AKT/MYC pathway[J]. J Exp Clin Cancer Res, 2019, 38(1): 95.
[9]Bennett JM, Catovsky D, Daniel MT, et al. Proposed revised criteria for the classification of acute myeloid leukemia. A report of the FrenchAmericanBritish Cooperative Group[J]. Ann Intern Med, 1985, 103(4): 620-625.
[10]Vardiman JW, Harris NL, Brunning RD. The World Health Organization (WHO) classification of the myeloid neoplasms[J]. Blood, 2002, 100(7): 2292-2302.
[12]Wang K, Wu Z, Si Y, et al. Identification of LPCAT1 expression as a potential prognostic biomarker guiding treatment choice in acute myeloid leukemia[J]. Oncol Lett, 2021, 21(2): 105.
[13]Luo X, Xu L, Wu X, et al. Decreased SATB1 expression promotes AML cell proliferation through NFκB activation[J]. Cancer Cell Int, 2019, 19: 134.
[14]ZareAbdollahi D, Safari S, Movafagh A, et al. Intact expression status of RASSF1A in acute myeloid leukemia[J]. Med Oncol, 2014, 31(1): 770.
[15]Pietrzak J, Mirowski M, Jeleń A, et al. Decreased MMP1 gene expression in acute myeloid leukaemia[J]. Mol Biol Rep, 2019, 46(2): 2293-2298.