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Effect of tetrandrine on human colorectal cancer HCT116 cell line and its potential mechanism
WANG Yue-xia1, ZHANG Zhi-jin2, ZHANG Yu-hao2, FU Jun2, QIN Xian-ju2
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Gastroenterology, Shanghai Eighth People′s Hospital, Shanghai 200235, China)
Abstract:Objective: To explore the effect of tetrandrine (TET) on human colorectal cancer HCT116 cell line and its potential mechanism. Methods: CCK8 assay was used to assess cells in viability and its 50% inhibitory concentration following treatment with TET. HCT116 cells were treated with different concentrations of TET (0, 2.5, 5 and 10 μmol/L), and cell colony formation assay was used to detect cell proliferation. Cell apoptosis was determined by using flow cytometry, mitochondrial membrane potential (MMP) assay and Hoechst 33342 fluorescent staining, and cell migration was determined by migration assay and Wound healing assay. The protein expression level of epithelial-mesenchymal transition marker protein and PI3K/AKT/NF-κB signal pathway after TET treatment was determined by Western blotting. Results: The activity of HCT116 cells decreased with the increase of TET concentration, and the 50% inhibition concentration of TET was 9.441 μmol/L. Compared with 0 μmol/L group, the number of colony forming cells in 2.5, 5 and 10 μmol/L TET groups decreased (all P<0.05), and the proportion of cells in G0/G1 phase increased, the proportion of cells in S phase and G2/M phase decreased in 5 and 10 μmol/L TET groups(all P<0.05). Compared with 0 μmol/L group, 2.5, 5 and 10 μmol/L TET group had more apoptosis and less migration ability (all P<0.05), and cells appeared round in 10 μmol/L TET group. Compared with 0 μmol/L group, the expression of Ecadherin was increased in 2.5, 5 and 10 μmol/L TET groups, while N-cadherin and Vimentin expression were decreased, and the phosphorylation of AKT and NFκB (p65), the key molecules in PI3K/AKT/NF-κB signaling pathway was inhibited(all P<0.05). Conclusion: TET may reverse the epithelial-mesenchymal transformation of HCT116 cells by inhibiting PI3K/Akt/NFκB signal pathway, and then reduce the ability of cell proliferation and migration and induce apoptosis.
基金资助:上海市徐汇区医学科研重大项目(SHXH201704)
通讯作者:
秦贤举(通讯作者),主任医师,教授,E-mail:qinxj@hotmail.com
作者简介: 王月霞(1992—),女,硕士研究生
引用本文:
王月霞1, 张治进2, 张玉浩2, 傅骏2, 秦贤举2. 粉防己碱对人结直肠癌HCT116细胞株生物功能的影响及其机制[J]. 江苏大学学报:医学版, 2020, 30(04): 336-341.
WANG Yue-xia1, ZHANG Zhi-jin2, ZHANG Yu-hao2, FU Jun2, QIN Xian-ju2.
#br#
Effect of tetrandrine on human colorectal cancer HCT116 cell line and its potential mechanism
. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 336-341.
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