Abstract：Objective To observe the relationship between blood pressure instant variability(BPIV), heart rate variability(HRV) and respiration in rabbits separately before and after hibateral vagus nerves cutting and respiratory arrest under respiratory rate regulation. Methods Ten standard experiment rabbits were selected and rabbit model of mechanical ventilation was established. The rabbits were conducted with mechanical ventilation under anesthesia by ventilator, with a constant tide volume of 10 mL/kg and respiratory rate of 70 times/min. Continuous recording of ECG, respiration and blood pressure was performed separately before and after hibateral vagus nerves cutting and respiratory arrest. The relationship between changes of HRV, BPIV and respiratory rate was analyzed. Results Before cutting hibateral vagus nerves, R-R interval and BP-BP were synchronized with the respiratory phase, with the same trend. The central frequencies of HRV and BPIV were both consistent with the central frequency of respiratory power spectral density. After cutting hibateral vagus nerves, the synchronous periodic change and trend consistency of R-R interval, BP-BP and respiration was destroyed; the central frequency of HRV and BPIV almost disappeared and was inconsistent with the central frequency of respiratory power spectral density; HRV and BPIV disappeared after respiratory arrest. Conclusion The changes of HRV and BPIV under respiratory rate regulation accord with the physiological mechanism of respiratory sinus arrhythmia. It is the secondary effect of R-R interval change which reflects the result of balance adjustment of autonomic nerve. It also coincides with the mechanism of HeringBreuer reflex induced by excitation of the tow receptor in the lung.
[1] Dick TE,Mims JR,Hsieh YH ,et al.Increased Cardio-respiratory coupling evoked by slow deep breathing can persist in normal humans[J].Respir Physiol Neurobiol,2014,204: 99-111.[2] 朱蕾,刘又宁,纽善福.临床呼吸生理学[M]. 北京：人民卫生出版社, 2008:227-234.[3] 朱妙章, 唐朝枢, 朱大年，等.心血管生理学基础与临床[M]. 北京:高等教育出版社, 2011:73-81, 104-121.[4] Malik M，Bigger JT，Camm AJ，et al．Heart rate variability: Standards of measurement，physiological interpretation，and clinical use[J]．Eur Heart J，1996，17( 3) : 354-381[5] 陆再英. 心率变异分析方法学的标准化及结果的正确评价[J]. 中国心脏起搏与心电生理杂志, 1996,10(4): 222-224.[6] Taralov ZZ, Terziyski KV, Kostianev SS. Heart Rate Variability as a Method for Assessment of the Autonomic Nervous System and the Adaptations to Different Physiological and Pathological Conditions[J].Folia Med (Plovdiv),2015,57(3-4):173-180.[7] Mancia G, Grassi G. Mechanisms and clinical implications of blood pressure variability[J]. J Cardiovasc Phaimacol, 2000, 35(7 Suppl 4):S15-19.[8] Parati G, Ochoa JE, Lombardi C, et al. Assessment and management of blood-pressure variability[J] . Nat Rev Cardiol, 2013 ,10 (3):143-155.[9] Gosmanova EO, Mikkelsen MK, Molnar MZ,et al, Association of Systolic Blood Pressure Variability With Mortality, Coronary Heart Disease, Stroke, and Renal Disease[J].J Am Coll Cardiol,2016,68(13):1375-1386.[10] 袁艳敏, 郭艺芳.人体血压昼夜节律特征及其调节机制[J]. 中华高血压杂志, 2008, 16(6) :483-485.[11] 血压变异性在临床中的意义—Giuseppe Mancia教授访谈[OL].国际循环网络版,2012,5. http://esh2012.icirculation.com/NewsView-24260-374-0.html.[12] 秦雪冰,俞森洋.家兔呼吸机所致肺损伤HSP70与IκBα表达的意义[J]. 心肺血管病杂志，2007，26(4):217-220.[13] 钟南山,王辰.呼吸内科学[M].北京:人民卫生出版社，2008：589-590.[14] 柴继红,吴水才,白燕萍,等. 心率变异性分析方法的现状与展望[J]. 生物医学工程与临床， 2004，8(1):53-60.[15] Olmstead AL, Kramer R, Dodam JR,et al. Sex hormone modulation of heart rate variability in miniature swine[J]. FASEB J， 2005,19:A618.[16] Poletto R, Janczak AM, MarchantForde RM,et al. Identification of low and high frequency ranges for heart rate variability and blood pressure variability analyses using pharmacological autonomic blockade with atropine and propranolol in swine[J]. Physiol Behav,2011,103(2): 188-196.[17] 沈仲元，Shin Lin，余平，等.调节呼吸频率对心率变异的影响[J].江苏实用心电学杂志, 2009, 18(5): 323-325.[18] 王步青,张政波,王卫东.引导呼吸下的呼吸性窦性心律不齐的研究[J].生物医学工程学杂志,2012,29(1):44-51.[19] ElOmar M, Kardos A, Casadei B. Mechanisms of respiratory sinus arrhythmia in patients with mild heart failure[J]. Am J Physiol Heart Circ Physiol, 2001, 280(1):H125-131.[20] 沈仲元，Shin Lin，阎世彪，等.家兔呼吸调节下血压即时变异与心率变异关系的研究[J].上海中医药杂志，2013，47(３):72-75.[21] Sun XG，Guo ZY．Decreased magnitudes of arterial. CO2 and CO2 oscillation explain CHeyneStokes periodic breatuing.Pattern in heart failure patients[J].FASEB J,2011,25：A847.[22] 孙兴国.整体整合生理学医学新理论体系概论Ⅰ：呼吸调控新视野[J].中国应用生理学杂志,2015,31(4):295-301.[23] 孙兴国.整体整合生理学医学新理论体系：人体功能一体化自主调控[J].中国循环杂志,2013,28（2）:88-92.[24] 贺书云，胡三觉,王贤辉，等.迷走神经在心率变异性中的作用[J].生理学报，2002,54（2）：129-132.[25] Warner MR, deTarnowsky JM, Whitson CC, et al. Beatbybeat modulation of AV conduction. II. Autonomic neural mechanisms[J]. Am J Physiol ,1986,251(6 Pt 2): H1134-H1142.[26] Harris MB,Milsom WK. Parasympathetic influence on heart rate in euthermic and hibernating ground squirrels[J]. J Exp Biol,1995,198(Pt 4): 931-937.[27] Perlini S, Sold PL, Piepoli M, et al. Determinants of respiratory sinus arrhythmia in the vagotomized rabbit[J]. Am J Physiol,1995,269:(3 Pt 2) H909-H915.
2017, Vol. 26 
