Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2024 Vol.34 Issue.02 Published 2024-03-22

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		2024 Vol. 34 (02): 0- [Abstract] ( 17 ) [HTML 1KB] [ PDF 708KB] ( 750 )

	93	Evaluation of renal function after renal transplantation by functional magnetic resonance imaging
		NI Bin1, ZHENG Ming2, WANG Ke3, ZHANG Junqi1, ZHANG Yudong4, HAN Zhijian2, TAO Jun2, JU Xiaobing2, TAN Ruoyun2, GU Min1,2, WANG Zijie2
		Objective： To investigate the value of functional magnetic resonance imaging (MRI) in evaluating the status of kidney grafts after renal transplantation. Methods： A total of 52 patients with stable renal function who underwent renal transplantation in the First Affiliated Hospital of Nanjing Medical University from January 2018 to December 2019 were selected as the study objects. The clearance rate of 99mTc DTPA was used as the reference glomerular filtration rate (rGFR), and the GFR of kidney grafts was estimated by dynamic contrast-enhanced （DCE）-MRI. The estimated DCE-MRI results were compared with rGFR using bias, accuracy, correlation, consistency and diagnostic analysis. Four groups of rat models were established: syngeneic graft group (SYN), renal ischemiareperfusion injury group (IRI), T cell mediated rejection group (TCMR) and antibody mediated rejection group (ABMR). The apparent diffusion coefficient (ADC) was measured by DWI sequence scanning on the 7th day after operation. After the examination, all rats were sacrificed immediately, and the transplanted kidney tissues were harvested for histological examination. Results： There was a significant positive correlation between the estimated GFR by DCE-MRI and rGFR (r=0.71, P<0.01). The consistency analysis showed that the bias was -3.544 mL/(min·1.73 m2), the accuracy was 15.33 mL/(min·1.73 m2), and the 95% confidence interval was 60.07 mL/(min·1.73 m2). DCE-MRI had an area under the curve of 0.91 with sensitivity of 79.17% and specificity of 82.14% in the diagnosis of patients with CKD stage 3 and above ［GFR<60 mL/(min·1.73 m2)］. Further animal experiments showed that there was no statistical difference in renal medulla ADC values between the four groups. In the meantime, renal cortex ADC values in SYN and IRI exhibit no significant difference. It′s worth noting that the cortical ADC values in TCMR group and ABMR group were significantly lower than those in SYN group (P<0.05), while there was no significant difference between TCMR group and ABMR group. In addition, the acute rejection group (TCMR group and ABMR group) had significantly lower renal cortical and medullary ADC values than those in the SYN group and IRI group (both P<0.05). Conclusion： Functional MRI could effectively and safely evaluate the renal function of patients after renal transplantation, with high sensitivity and specificity. In animal experiments, acute rejection after kidney transplantation can be well diagnosed by functional MRI.
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		2024 Vol. 34 (02): 93-98 [Abstract] ( 14 ) [HTML 1KB] [ PDF 1930KB] ( 342 )

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		2024 Vol. 34 (02): 99-103,110 [Abstract] ( 13 ) [HTML 1KB] [ PDF 816KB] ( 306 )

	104	The gene expression profiles of Henle-loop cell subset in renal tissue of the calcium oxalate kidney stone rat model based on single-cell sequencing analysis
		XU Ting1, ZHANG Ying1, DENG Qiong1,2, LIANG Hui1, WANG Zhu1,2
		Objective： To explore the gene expression profiles of Henle-loop cell subset in renal tissue of the calcium oxalate kidney stone rat model. Methods： The rat model of calcium oxalate kidney stone was constructed by ethylene glycol and 1% ammonium chloride. The model was validated by Von Kossa′s staining. Henle-loop cells were identified using single-cell sequencing method. The gene expression profiles of Henle-loop cell subsets in renal tissue of calcium oxalate kidney stone rat model were performed via comparative analysis. The enrichment analysis of differential genes was performed through GO, KEGG, and GSEA bioinformatics tools. Results： Compared with the control group, black or brownish black staining of calcium salt deposition were observed in the kidney tissue of the experimental group rats, indicating the successful construction of the calcium oxalate kidney stone rat model. Single cell sequencing analysis revealed that there was a total of 3 510 differentially expressed genes in the Henle-loop cell subset of the experimental group, of which 880 were upregulated and 2 630 were downregulated. LOC499584, Il7, dual specificity phosphatase 1 (Dusp1), and matrix Gla protein (Mgp) were upregulated most significantly, while HORMA domain containing 2 (Hormad2), LOC361914, Jun dimerization protein 2 (Jdp2), and somatomedinB and thrombospondin type-1 domain-containing protein (Sbspon) were downregulated most significantly. KEGG analysis revealed significant changes in signaling pathways such as oxidative phosphorylation, metabolism pathways, propanoate metabolism, autophagy, lysosome and endocytosis in the experimental group of Henle-loop cells. Conclusion： This study reveals for the first time the gene expression profile characteristics of Henleloop cell subsets in renal tissue of the calcium oxalate kidney stone rat model, providing a molecular basis for the investigation of kidney stone formation and related kidney injury.
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		2024 Vol. 34 (02): 104-110 [Abstract] ( 16 ) [HTML 1KB] [ PDF 9420KB] ( 289 )

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		2024 Vol. 34 (02): 111-117 [Abstract] ( 15 ) [HTML 1KB] [ PDF 813KB] ( 278 )

	118	Effect of KDEL endoplasmic reticulum protein retension receptor 2 on the malignant growth of pancreatic cancer and potential mechanisms
		WANG Shuhang1, WU Xiaoyang2
		Objective： To investigate the effect of KDELR2 on the malignant growth of pancreatic cancer cells, and its underlying molecular mechanisms. Methods： The expression of KDELR2 mRNA in pan-cancer and human pancreatic cancer tissues was analyzed by UCSC XENA database, and the association of its expression with clinicopathological characteristics and prognosis of pancreatic cancer patients was analyzed based on TCGA database. GO and KEGG analyses were used to explore the potential enriched signaling pathways of KDELR2related differentially expressed genes (DEGs). qRT-PCR and Western blotting were respectively applied to detect the KDELR2 mRNA and protein expression levels in different pancreatic cancer cell lines (BXPC-3, MIA PaCa-2, PANC-1, and PaTu 8988-T), as well as normal HPNE, and the KDELR2 protein level of human pancreatic cancer tissues and paracancerous tissues were also tested. Stable pancreatic cancer cells (PaTu 8988-T and PANC-1) containing the lentiviral particles encoding KDELR2 cDNA or KDELR2 shRNA and their scramble control lentiviral particles (“KDELR2-OE” and “Vec”, “shKDELR2S1/S2”and “shNC”) were formed. In addition, CCK-8, EdU staining, Transwell, and cell colony formation experiments were applied to observe the functional change of those stable cell lines. The mitochondrial function of PaTu 8988-T and PANC-1 cells after KDELR2 knockdown was observed by JC-1, MitoSOX and DCFH-DA staining assays and ATP level detection assays respectively. Furthermore, the effect of KDELR2 shRNA on the growth of PANC-1 cells in vivo was explored by constructing a subcutaneous xenograft tumor model in nude mice. Results： KDELR2 was overexpressed in human pancreatic cancer tissues and cells, and its overexpression was positively correlated with higher tumor clinicopathological stages, indicating a poor prognosis of pancreatic cancer patients. GO function and KEGG enrichment analysis suggested that KDELR2 may regulate the growth of pancreatic cancer cells by regulating cellular energy metabolism. After silencing of KDELR2, the growth, proliferation and migration of pancreatic cancer cells were significantly inhibited. Ectopic overexpression of KDELR2 significantly promoted the proliferation and migration of pancreatic cancer cells. After KDELR2 knockdown, pancreatic cancer cells showed a significant decrease in mitochondrial membrane potential and ATP levels, a significant increase in reactive oxygen species levels (all P<0.05). A subcutaneous xenograft model in nude mice demonstrated that KDELR2 knockdown significantly inhibited the growth of PANC-1 cells in vivo. Conclusion： KDELR2 is overexpressed in pancreatic cancer tissues and correlated with poor prognosis of pancreatic cancer patients, and KDELR2 may be involved in regulating mitochondrial function and energy metabolism in cancer cells.
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		2024 Vol. 34 (02): 118-131 [Abstract] ( 14 ) [HTML 1KB] [ PDF 8609KB] ( 290 )

	132	Dynamic expression and significance of Foxp3 in the development of mouse lung
		JIANG Jianfeng, LU Hongyan, ZHU Yue, HE Langyue, ZHU Ying
		Objective： To explore the possible role of forkhead box protein 3 (Foxp3) in lung development by analyzing the dynamic expression of Foxp3 during mouse lung development and its relationship with lung development related markers. Methods： According to the process of lung development, fetal and newborn mice were divided into 6 groups. Lung tissues were taken at 17.5 days of pregnancy and 1 day, 4 days, 7 days, 14 days and 21 days after birth. Lung morphology was observed by HE staining, CD31 content was detected by immunohistochemical staining and pulmonary microvessel density was observed. The expression of Foxp3 and surfactant protein C (SPC), vascular endothelial growth factor A (VEGFA), angiopoietin 1 (Ang-1) mRNA was detected by qRT-PCR, and the protein expression of Foxp3 and SP-C, VEGF-A, Ang-1 was detected by Western blotting. Results： The expression of Foxp3 mRNA in lung tissue was the highest in the tubule stage at E175 d, and then decreased continuously. The expression of SP-C mRNA was the highest at 1 day after birth, and then decreased gradually until the late stage of alveolization. The expression of VEGF-A and Ang-1 mRNA was the highest at E17.5 d, then decreased gradually, and finally tended to be stable. The expression of Foxp3 protein was found in the tubule stage, and the expression was the highest in the tubule phase and vesicular phase, and then gradually stabilized. The expression of VEGF-A and Ang-1 was also the highest in the tubule and vesicular phase, and then decreased gradually, while the expression of SP-C reached the peak at 1 day after birth, then decreased gradually, and tended to be stable in the middle and late stage of alveoli. Correlation analysis showed that Foxp3 was correlated with SP-C, VEGF-A and Ang-1 (r=0.661, 0.630 and 0.738). Conclusion： Foxp3 showed dynamic expression in fetal lung and early postnatal stage. The high expression of Foxp3 in tubule stage and early and middle vesicle stage was positively correlated with SP-C, VEGF-A and Ang-1, suggesting that Foxp3 may promote the proliferation of alveolar epithelial cells and pulmonary vascular endothelial cells and participate in the process of lung development.
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		2024 Vol. 34 (02): 132-137 [Abstract] ( 13 ) [HTML 1KB] [ PDF 3812KB] ( 220 )

	138	Effect of human umbilical cord mesenchymal stem cell-derived extracellular vesicles on house dust mite-induced airway inflammation in asthmatic mice
		LUO Xinkai, ZHENG Tingting, DONG Liyang, XU Xiaowei, GAO Xuerong, GU Weifeng, MAO Chaoming
		Objective： To investigate the effect of human umbilical cord mesenchymal stem cells-derived extracellular vesicles (hUCMSC-EVs) on house dust mite (HDM)-induced airway inflammation in asthmatic mice. Methods： hUCMSC-EVs was extracted by ultra-high speed centrifugation; morphological characteristics of hUCMSC-EVs were observed by transmission electron microscopy. EVs markers, tumor susceptibility gene 101 (TSG101) and heat shock protein 70 (HSP70), were detected by Western blotting. Fifteen female BALB/C mice were randomly divided into 3 groups, 5 mice in each group, including control group (without any treatment), HDM group (HDM treatment) and HDM+EVs group (HDM+hUCMSC-EVs treatment). Hematoxylin and eosin (HE) and periodic acid--Schiff (PAS) staining were used to observe the inflammatory cell infiltration and goblet cell hyperplasia of lung airway in each group. Bronchoalveolar lavage fluid (BALF) was collected, and the numbers of total cells and eosinophils were counted. The contents of IL-4 and IL-13 in BALF were detected by ELISA. The expression of E-cadherin and atresia connexin 1(ZO-1) in the lungs was detected by Western blotting. Human bronchial epithelial BEAS-2B cell lines were divided into control group and EVs group (DIOlabeled hUCMSC-EVs treatment). Confocal microscopy was used to observe the endocytosis of DIO-labeled hUCMSC-EVs cells. BEAS2B cells were divided into three groups: control group, HDM group (HDM treatment), and HDM+EVs group (HDM+hUCMSC-EVs treatment), and the protein expression of E-cadherin and ZO-1 in the cells was detected by Western blotting. Results： hUCMSC-EVs showed a circular membrane structure and expressed HSP70 and TSG101. Compared with HDM group, airway inflammatory score, PAS score, the number of total cells and eosinophils in BALF, the contents of IL-4 and IL-13 in BALF were significantly decreased in HDM+EVs group (all P<0.01), whereas E-cadherin and ZO-1 expressions in lung tissues were markedly increased (P<0.05). In vitro experiments showed that hUCMSC-EVs could be endocytosed by human bronchial epithelial cell line BEAS-2B. Compared with HDM group, the expressions of E-cadherin and ZO-1 were significantly increased in HDM+EVs group (P<0.01 or <0.05). Conclusion： hUCMSC-EVs could ameliorate HDM-induced peri-airway inflammatory cell infiltration and airway goblet cell hyperplasia in mice.
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		2024 Vol. 34 (02): 138-144 [Abstract] ( 13 ) [HTML 1KB] [ PDF 5298KB] ( 312 )

	145	Efficacy and safety of EndoClot intraoperative hemostatic device in Bama miniature pigs heparinized active gastrointestinal ulcer bleeding model
		KE Qili1, ZHANG Heng2, XING Cheng2, LIU Hongfei1
		Objective： To evaluate the efficacy and safety of EndoClot intraoperative hemostatic device for the active bleeding model of heparinized digestive tract ulcer in Bama miniature pigs. Methods： Twelve healthy Bama miniature pigs weighing 40-45 kg were selected and randomly divided into control group ［EndoClot polysaccharide hemostatic system (PHS)］ and experiment group (EndoClotintraoperative hemostatic device), with 6 pigs in each group. The active bleeding model of heparinized digestive tract (upper gastrointestinal tractstomach; lower gastrointestinal tractcolon) was performed. Two ulcer active bleeding wounds were created in each pig, one in each upper and lower digestive tract. The success rate of hemostasis during operation, the time to achieve effective hemostasis, the total amount of hemostatic powder and the wound healing at different time after operation (7, 14, 21, 28 d) were observed and compared between the two groups. Pathological evaluation was performed on stomach, pancreas, lymph nodes, intestinal wounds and brain tissues of the two groups by HE staining. Results： Compared with the control group (11/12, 91.7%), the success rate of hemostasis in the experiment group (12/12, 100.0%) was higher, and the average time to achieve effective hemostasis was shorter and the used amount of hemostatic powder was less, but the differences were not statistically significant (all P>0.05). Compared with the control group, the wound healing score of the experiment group was significantly increased on the 7th and 14th day after surgery (P<0.05), and there was no statistical difference between the two groups on the 21st and 28th day (P>0.05). HE staining showed that no tissue necrosis, foreign body granuloma, ischemia, embolism, capillary hyperplasia, fibrous envelope formation and fat infiltration were observed in the stomach, pancreas, lymph nodes, intestinal wounds and brain in the control group and the experimen group. Conclusion： EndoClot intraoperative hemostatic device could realize the hemostasis of active bleeding of heparinized gastrointestinal ulcer in Bama miniature pigs, which is safe, effective and convenient to operate.
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		2024 Vol. 34 (02): 145-150 [Abstract] ( 14 ) [HTML 1KB] [ PDF 5563KB] ( 348 )

	151	Drop-off ddPCR-based method for detecting mutations in C-KIT gene N822 locus and its clinical application in acute myeloid leukemia
		LI Ting1, JIN Ye1, YUAN Qian2,3, YAO Dongming2,3, XIANG Helin1, XIAO Gaofei2,3, YU Di1, LENG Jiayan1, LIN Jiang2,3, QIAN Jun1,2,3
		Objective： To develop a quantitative drop-off ddPCR technique for identifying of mutations at the C-KIT gene′s N822 locus in patients with acute myeloid leukemia (AML), and assess the methodological performance and its clinical value. Methods： A pair of primers and probes were designed for the exon 17 of C-KIT gene. The system and reaction conditions of drop-off ddPCR were optimized. The method′s specificity, sensitivity, and repeatability were assessed. The established method was used to test bone marrow samples from 140 newly diagnosed AML patients previously Sanger sequenced, and the results were further confirmed by next generation sequencing (NGS). In addition, the KIT-N822 mutation frequency was monitored dynamically in 3 patients by dropoff ddPCR. Results： The optimal annealing temperature of drop-off ddPCR for the KIT-N822 mutation was 54 ℃. With good linearity, the limit of detection was 10.12 copies/μL, and the limit of blank was 1.62 copies/μL. Among 140 AML samples, Sanger sequencing detected only 2 positive cases (1.43%), however, ddPCR identified 7 positive (5.00%) with mutation frequency of 0.29%~7.41%, among which NGS found only 3 positive cases (2.1%) with variant allele frequencies ranging from 1.26% to 8.00%. The results showed that the C-KIT mutation frequency decreased significantly or even to 0 when the treatment reached complete remission. Conclusion： The drop-off ddPCR method for detecting KIT-N822 mutations was developed with better methodological performance and higher sensitivity than both Sanger sequencing and NGS. It maybe employed for monitoring measurable residual disease and guiding treatment following remission in positive patients.
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		2024 Vol. 34 (02): 151-155,160 [Abstract] ( 15 ) [HTML 1KB] [ PDF 4233KB] ( 409 )

	156	Level of fecal calprotectin and lactoferrin in patients with Parkinson′s disease and their correlation with serum IL-6 and TNF-α levels
		ZHAO Jing, SHU Yu, QIAN Jinjun
		Objective： To explore the level of fecal calprotectin (CPT) and lactoferrin (LF) in patients with Parkinson′s disease (PD), and analyze their correlation with clinical symptoms of PD and serum inflammatory factors. Methods： A total of 55 PD patients who first visited the Department of Neurology, the Fourth Affiliated Hospital of Jiangsu University from January 2019 to January 2022 were selected (PD group), and 48 healthy subjects during the same period (control group) were enrolled in the study. The general data of the two groups were collected and compared, and the fecal CPT and LF levels of the two groups as well as the serum tumor cell necrosis factorα (TNFα) and interleukin6 (IL6) levels of the PD group were detected. After 2 months of treatment, fecal CPT and LF levels in the PD group were measured again. The PD group was scored on the Unified Parkinson′s Disease Rating Scale (UPDRS) before and after treatment. Pearson correlation was used to analyze the correlation between fecal CPT, LF levels and UPDRS scores in the PD group, as well as the correlation between ΔCPT, ΔLF and ΔUPDRS scores. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of fecal CPT and LF levels for PD. Results： There were no significant differences in clinical characteristics between PD group and control group (all P>0.05). Before treatment, the fecal levels of CPT and LF in PD group were significantly higher than those in control group (all P<0.01). After treatment, the fecal levels of CPT and LF in PD group, as well as the UPDRS score, were significantly declined compared with those before treatment (all P<0.01). The UPDRS score had a positive correlation with fecal CPT and LF (r=0.605, 0.619, both P<0.01). ΔUPDRS score had a positive correlation with ΔCPT and ΔLF (r=0.735, 0.760, both P<0.01). The fecal CPT and LF levels in PD group before treatment were positively correlated with serum TNF-α and IL-6 respectively (CPT: rTNF-α=0.689, rIL-6=0.591; LF: rTNF-α=0.673, rIL-6=0.616; all P<0.01). The areas under the ROC curves of fecal CPT and LF were 0.892 and 0.874, the sensitivities were 89.58% and 88.32%, and the specificities were 83.29% and 74.55%, respectively. Conclusion： Fecal CPT and LF levels are increased in PD patients, which is correlated with symptom scores and serum TNF-α and IL-6 levels.
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		2024 Vol. 34 (02): 156-160 [Abstract] ( 14 ) [HTML 1KB] [ PDF 1465KB] ( 317 )

	161	Correlation analysis of serum levels of small dense low-density lipoprotein cholesterol and total antioxidant capacity with cognitive dysfunction in patients with cerebral infarction
		MA Juan, HAO Chenguang, HE Dan, MA Jianhua
		Objective： To investigate the serum small dense low-density lipoprotein cholesterol (sdLDL-C) and total antioxidant capacity (T-AOC) in patients with cerebral infarction and its relationship with cognitive dysfunction. Methods： A total of 148 patients with cerebral infarction were selected and divided into non-impaired group (n=77) and impaired group (n=71) according to whether cognitive dysfunction occurred 7 days after onset. The levels of serum sdLDL-C and T-AOC in the two groups were determined by hydroperoxidase and colorimetry, respectively, and compared. The correlation between serum sdLDL-C, T-AOC and Montreal Cognitive Assessment (MoCA) score in patients with cerebral infarction was analyzed by Pearson correlation coefficient method. The risk factors of cognitive dysfunction in patients with cerebral infarction were analyzed by binary Logistic regression. Results： Compared with the non-impaired group, the serum sdLDL-C level in the impaired group was significantly increased (P<0.05), and the T-AOC level was greatly decreased (P<0.05). Correlation analysis showed that serum sdLDL-C level was negatively correlated with MoCA score (r=-0.516, P<0.05), while T-AOC level was positively correlated with MoCA score (r=0.446, P<0.05). Logistic regression analysis showed that hypertension, diabetes, disease severity, serum sdLDL-C and T-AOC were independent factors affecting the occurrence of cognitive dysfunction in patients with cerebral infarction (P<0.05). Conclusion： The level of serum sdLDL-C is increased and the level of T-AOC is decreased in patients with cerebral infarction, which are independent influencing factors for cognitive dysfunction in patients with cerebral infarction.
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		2024 Vol. 34 (02): 161-165,175 [Abstract] ( 16 ) [HTML 1KB] [ PDF 898KB] ( 315 )

	166	Screening of potential chromatin regulators under immune infiltration in irritable bowel syndrome based on bioinformatics
		PENG Jianlan, ZHU Yongping, LIN Shouning, LIAO Dongyan, JIANG Junling, WU Yuexia
		Objective： To screen out potential chromatin regulators (CRs) under immune infiltration in irritable bowel syndrome (IBS) by bioinformatics analysis and to evaluate their links with immune cells and immune functions. Methods： The CRs file and IBS gene expression matrix were integrated, and weighted gene coexpression analysis and differential analysis were performed to take the intersection CRs; based on the CRs obtained from the intersection, proteinprotein interaction (PPI) analysis was performed and the network diagram was constructed. Immune infiltration was extracted and quantified using a gene expression matrix to analyze the correlation and variability between immune cells and between immune functions; and the correlation with CRs in the PPI network diagram was analysed. By constructing a risk model, we analyzed the risk probability of CRs closely related to immune infiltration, and thus screened out the potential CRs. Results： PPI analysis identified a total of 13 interacting CRs in IBS. Among immune cells, the correlation ship between B cells and follicular T helper cells was the strongest and positively correlated (r=0.90). In immune function, the correlation ship between checkpoint and T cell coinhibition was the strongest and positively correlated (r=0.89). Compared with healthy subjects, IBS patients showed a significant increase in co-stimulation of dendritic cells, immature dendritic cells, Th1 cells, and antigen presentation (all P<0.05). The correlation analysis between CRs and immune infiltration showed that SIN3 transcription regulator family member B gene (SIN3B) was positively correlated with natural killer cells, serine/arginine repetitive matrix protein 2 (SRRM2) was positively correlated with Th1 cells and antigen--presenting cells co-stimulation, while DPY30 was negatively correlated with helper T cells and Th1 cells. Risk model results showed that SIN3B and SRRM2 may be independent risk factors for IBS. Conclusion： SIN3B and SRRM2 are potential CRs for immune infiltration in IBS, which may regulate the occurrence and development of IBS by regulating natural killer cells, Th1 cells and antigen presentation co-stimulation.
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		2024 Vol. 34 (02): 166-175 [Abstract] ( 17 ) [HTML 1KB] [ PDF 16361KB] ( 283 )

	176	Clinical characteristics and risk factors of non-cuffed catheter-related bloodstream infection in hemodialysis patients
		XU Lihua, HE Jianqiang
		Objective： To explore the clinical characteristics and risk factors of catheterrelated bloodstream infection (CRBSI) in patients undergoing hemodialysis with non-cuffed catheter (NCC). Methods： A retrospective analysis was performed on 169 patients who underwent hemodialysis treatment with ultrasound-guided percutaneous central venous insertion of non-cuffed catheter at the Blood Purification Center, Affiliated Hospital of Jiangsu University from January 2019 to December 2021. The incidence of CRBSI at different catheterization sites was analyzed, and the infection characteristics and pathogenic bacteria distribution of CRBSI patients were analyzed. The patients were divided into infected group (n=22) and noninfected group (n=147) according to whether CRBSI occurred, and the clinicopathological parameters of the two groups were compared. Further, Logistic regression was used to analyze the influencing factors of CRBSI in NCC patients,and receiver operating characteristic (ROC) curve was used to evaluate the prediction effect. Results： There was no significant difference in incidence of CRBSI between internal jugular vein catheterization and femoral vein catheterization (χ2=0.221, P=0.638). A total of 21 strains of pathogenic bacteria were detected in 22 patients with CRBSI, including 16 strains of Gram-positive coccus, mainly Staphylococcus aureus (10 strains) and 5 strains of Gram-negative bacillus. The levels of serum albumin, high-density lipoprotein and serum iron in infected group were significantly lower than those in non-infected group (all P<0.05). Logistic regression analysis showed that serum albumin and high-density lipoprotein were influencing factors for CRBSI in hemodialysis patients (P=0.006, 0.007). ROC curve showed that the AUC of albumin and high-density lipoprotein was 0.726 and 0.704, respectively. Conclusion： Gram-positive bacteria, especially staphylococcus aureus, were the main pathogens causing CRBSI in non-cuffed catheter hemodialysis patients. The low levels of serum albumin and high-density lipoprotein are risk factors for CRBSI.
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		2024 Vol. 34 (02): 176-179,184 [Abstract] ( 16 ) [HTML 1KB] [ PDF 997KB] ( 298 )

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		2024 Vol. 34 (02): 180-184 [Abstract] ( 15 ) [HTML 1KB] [ PDF 764KB] ( 377 )

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