Foxp3在小鼠肺发育中的动态表达及意义

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (3812 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS) Supporting Info

Guide

Abstract Objective： To explore the possible role of forkhead box protein 3 (Foxp3) in lung development by analyzing the dynamic expression of Foxp3 during mouse lung development and its relationship with lung development related markers. Methods： According to the process of lung development, fetal and newborn mice were divided into 6 groups. Lung tissues were taken at 17.5 days of pregnancy and 1 day, 4 days, 7 days, 14 days and 21 days after birth. Lung morphology was observed by HE staining, CD31 content was detected by immunohistochemical staining and pulmonary microvessel density was observed. The expression of Foxp3 and surfactant protein C (SPC), vascular endothelial growth factor A (VEGFA), angiopoietin 1 (Ang-1) mRNA was detected by qRT-PCR, and the protein expression of Foxp3 and SP-C, VEGF-A, Ang-1 was detected by Western blotting. Results： The expression of Foxp3 mRNA in lung tissue was the highest in the tubule stage at E175 d, and then decreased continuously. The expression of SP-C mRNA was the highest at 1 day after birth, and then decreased gradually until the late stage of alveolization. The expression of VEGF-A and Ang-1 mRNA was the highest at E17.5 d, then decreased gradually, and finally tended to be stable. The expression of Foxp3 protein was found in the tubule stage, and the expression was the highest in the tubule phase and vesicular phase, and then gradually stabilized. The expression of VEGF-A and Ang-1 was also the highest in the tubule and vesicular phase, and then decreased gradually, while the expression of SP-C reached the peak at 1 day after birth, then decreased gradually, and tended to be stable in the middle and late stage of alveoli. Correlation analysis showed that Foxp3 was correlated with SP-C, VEGF-A and Ang-1 (r=0.661, 0.630 and 0.738). Conclusion： Foxp3 showed dynamic expression in fetal lung and early postnatal stage. The high expression of Foxp3 in tubule stage and early and middle vesicle stage was positively correlated with SP-C, VEGF-A and Ang-1, suggesting that Foxp3 may promote the proliferation of alveolar epithelial cells and pulmonary vascular endothelial cells and participate in the process of lung development.

Key words：

Received: 09 May 2023

Fund:

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	JIANG Jianfeng
	LU Hongyan
	ZHU Yue
	HE Langyue
	ZHU Ying

Cite this article:

JIANG Jianfeng,LU Hongyan,ZHU Yue等. Dynamic expression and significance of Foxp3 in the development of mouse lung[J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(02): 132-137.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2024/V34/I02/132

［1］Schittny JC. Development of the lung［J］. Cell Tissue Res, 2017, 367(3): 427-444.

［2］Barkauskas CE, Cronce MJ, Rackley CR, et al. Type 2 alveolar cells are stem cells in adult lung［J］. J Clin Invest, 2013, 123(7): 3025-3036.

［3］DeLisser HM, ChristofidouSolomidou M, Strieter RM, et al. Involvement of endothelial PECAM1/CD31 in angiogenesis［J］. Am J Pathol, 1997, 151(3): 671-677.

［4］Chinoy MR, Graybill MM, Miller SA, et al. Angiopoietin1 and VEGF in vascular development and angiogenesis in hypoplastic lungs［J］. Am J Physiol Lung Cell Mol Physiol, 2002, 283(1): L60-L66.

［5］Lam EW, Brosens JJ, Gomes AR, et al. Forkhead box proteins: tuning forks for transcriptional harmony［J］. Nat Rev Cancer, 2013, 13(7): 482495.

［6］Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells［J］. Nat Immunol, 2003, 4(4): 330-336.

［7］Zacchigna S, Martinelli V, Moimas S, et al. Paracrine effect of regulatory T cells promotes cardiomyocyte proliferation during pregnancy and after myocardial infarction［J］. Nat Commun, 2018, 9(1): 2432.

［8］Zhong Q, Jenkins J, Moldobaeva A, et al. Effector T cells and ischemiainduced systemic angiogenesis in the lung［J］. Am J Respir Cell Mol Biol, 2016, 54(3): 394-401.

［9］Yang S, Liu Y, Li MY, et al. Foxp3 promotes tumor growth and metastasis by activating Wnt/βcatenin signaling pathway and EMT in nonsmall cell lung cancer［J］. Mol Cancer, 2017, 16(1): 124.

［10］Pan H, Deutsch GH, Wert SE, et al. Comprehensive anatomic ontologies for lung development: A comparison of alveolar formation and maturation within mouse and human lung［J］. J Biomed Semantics, 2019, 10(1): 18.

［11］Meng X, Cui G, Peng G. Lung development and regeneration: newly defined cell types and progenitor status［J］. Cell Regen, 2023, 12(1): 5.

［12］唐兴萍, 李坚, 汪毅. 非小细胞肺癌患者外周血中生存素和血管内皮细胞生长因子的表达及临床意义［J］. 江苏大学学报(医学版), 2013, 23(1): 34-38.

［13］王玲, 封志纯, 吕回. 血管内皮生长因子和血管生成素1在高氧诱导新生鼠支气管肺发育不良的表达及其对肺发育的影响［J］. 实用医学杂志, 2014, 30(4): 525-528.

［14］鲍俊峰, 王胜军, 马洁, 等. 小鼠Foxp3蛋白的原核表达及多克隆抗体的制备［J］. 江苏大学学报(医学版), 2008, 18(4): 310-313.

［15］Li J, Yang KY, Tam RCY, et al. Regulatory Tcells regulate neonatal heart regeneration by potentiating cardiomyocyte proliferation in a paracrine manner［J］. Theranostics, 2019, 9(15): 4324-4341.

［16］贺欣然, 孙瑜. 胎儿肺脏发育及其调节机制［J］. 中华围产医学杂志, 2015, 18 (10): 794-797.

［17］D′Alessio FR, Zhong Q, Jenkins J, et al. Lung angiogenesis requires CD4+ forkhead homeobox protein3+ regulatory Tcells［J］. Am J Respir Cell Mol Biol, 2015, 52(5): 603-610.

［18］Mock JR, Garibaldi BT, Aggarwal NR, et al. Foxp3+ regulatory T cells promote lung epithelial proliferation［J］. Mucosal Immunol, 2014, 7(6): 1440-1451.

［19］Zhong Q, Jenkins J, Moldobaeva A, et al. Effector T cells and ischemiainduced systemic angiogenesis in the lung［J］. Am J Respir Cell Mol Biol, 2016, 54(3): 394-401.

[1]	. [J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(02): 99-103,110.