基于单细胞测序的草酸钙肾结石大鼠髓袢细胞亚群基因表达谱特征分析

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Abstract Objective： To explore the gene expression profiles of Henle-loop cell subset in renal tissue of the calcium oxalate kidney stone rat model. Methods： The rat model of calcium oxalate kidney stone was constructed by ethylene glycol and 1% ammonium chloride. The model was validated by Von Kossa′s staining. Henle-loop cells were identified using single-cell sequencing method. The gene expression profiles of Henle-loop cell subsets in renal tissue of calcium oxalate kidney stone rat model were performed via comparative analysis. The enrichment analysis of differential genes was performed through GO, KEGG, and GSEA bioinformatics tools. Results： Compared with the control group, black or brownish black staining of calcium salt deposition were observed in the kidney tissue of the experimental group rats, indicating the successful construction of the calcium oxalate kidney stone rat model. Single cell sequencing analysis revealed that there was a total of 3 510 differentially expressed genes in the Henle-loop cell subset of the experimental group, of which 880 were upregulated and 2 630 were downregulated. LOC499584, Il7, dual specificity phosphatase 1 (Dusp1), and matrix Gla protein (Mgp) were upregulated most significantly, while HORMA domain containing 2 (Hormad2), LOC361914, Jun dimerization protein 2 (Jdp2), and somatomedinB and thrombospondin type-1 domain-containing protein (Sbspon) were downregulated most significantly. KEGG analysis revealed significant changes in signaling pathways such as oxidative phosphorylation, metabolism pathways, propanoate metabolism, autophagy, lysosome and endocytosis in the experimental group of Henle-loop cells. Conclusion： This study reveals for the first time the gene expression profile characteristics of Henleloop cell subsets in renal tissue of the calcium oxalate kidney stone rat model, providing a molecular basis for the investigation of kidney stone formation and related kidney injury.

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Received: 18 December 2023

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	Articles by authors
	XU Ting1
	ZHANG Ying1
	DENG Qiong1
	2
	LIANG Hui1
	WANG Zhu1
	2

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XU Ting1,ZHANG Ying1,DENG Qiong1等. The gene expression profiles of Henle-loop cell subset in renal tissue of the calcium oxalate kidney stone rat model based on single-cell sequencing analysis[J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(02): 104-110.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2024/V34/I02/104

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[1]	. [J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(02): 99-103,110.