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Journal of Jiangsu University(Medicine Edition)
 
2020 Vol.30 Issue.01
Published 2020-01-30
1 Acinetobacter baumannii capsular promotes autophagy and apoptosis of murine macrophages by inhibiting PI3K/Akt/mTOR signaling pathway
HAN Chang-ming1,2, DAI Xiao-yue1, DUAN Qian-mei2, QIU Sheng-gang2, SHI Li-xin2,XIA Wen1, ZOU Zhi-qing1, Dinsh Kumar K1, WU Liang1, YIN Qing3, XU Hua-xi 1
[Abstract\]Objective: To study the capability of Acinetobacter baumannii with different thickness capsules induce Raw 264.7 cell autophagy and apoptosis, and its potential mechanisms. Methods: A baumannii was isolated from the sputum of patients with pneumonia. The bacterial capsule was stained by Congo red staining, and the bacterial strains with significantly different capsule thickness were selected for subsequent study. Raw 264.7 cells were infected with thick capsular strain (AbH strain) or thin capsular strain (AbB strain), and the cells were harvested at 1 h, 3 h and 6 h after infection. The expression of autophagyrelated proteins LC3 and Beclin1, as well as the phosphorylation levels of PI3K, Akt and mTOR were determined by Western blotting. Flow cytometry was used to determine the reactive oxygen species(ROS) production and apoptosis rate. Results: After A. baumannii was stained with Congo red, the cells were shown purpleblack, the background was red, and the capsule was not stained. The bacterial capsule thickness was clearly judged. Two strains with significant differences in capsular thickness were grouped, namely thick capsular strain (AbH strain) and thin capsular strain (AbB strain). The expression of autophagyrelated protein Beclin1 of Raw 264.7 cells induced by AbH strain were significantly higher than that of AbB strain (P<0.05), and the apoptosis rate of Raw 264.7 cells induced by AbH strain was also significantly higher than that of AbB strain (P<0.05). Two strains of A. baumannii could induce a significant increase in the production of ROS in Raw 264.7 cells (P<0.05), and the ability of AbH strain to induce ROS production was significantly higher than that of AbB strains (P<0.05). After Raw 264.7 cells were infected with two strains respectively, the phosphorylation levels of PI3K, Akt and mTOR were significantly inhibited, and the inhibition increased when the time prolonged (P<0.05). Conclusion: A. baumannii could induce autophagy and apoptosis of Raw 264.7 cells, and the induction ability is positively correlated with bacterial capsule thickness. A. baumannii with thick capsule has a stronger ability to induce ROS production in Raw 264.7 cells and inhibit the activation of PI3K/Akt/mTOR signaling pathway, which ultimately enhances autophagy and apoptosis of Raw 264.7 cells.

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2020 Vol. 30 (01): 1- [Abstract] ( 37 ) [HTML 1KB] [ PDF 2312KB] ( 624 )
7
The sensitization effect of RNAi silenced Sox2 expression on 5-FU in colorectal cancer cells
The sensitization effect of RNAi silenced Sox2 expression on 5-FU in colorectal cancer cells
[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 7-11,17>')" href="#">		 YUN Ya-jing1, XU Li-Xiao2, SUN Bin1, FENG Xing1
[Abstract]Objective: To investigate the relationship between Sox2 and 5fluorouracil (5FU) induced chemotherapy sensitivity of human colon cancer cells (SW620) and gene expression in drugresistant families,and to explore the mechanism of chemotherapy resistance in colorectal cancer cells. Methods: After silencing SW620 cells with lentiviral packaging interference plasmid Sox2, Western blot, RTPCR and immunofluorescence staining were used to detect the changes in the expression of Sox2 in cells before and after interference,CCK8 assay was used to detect the sensitivity of cells to 5FU,Flow cytometry was used to detect the apoptosis rate of cells and the expression of drugresistant family genes. Results: After targeting Sox2 interference, the expression of Sox2 mRNA and protein was downregulated, and the positive expression rate of Sox2 was significantly reduced. After interfering with Sox2, the sensitivity of SW620 cells to 5FU was higher than that of control cells,the IC50 value decreased significantly,and there was a significant statistical difference between cells (P<0.05).The apoptosis rate of cells after Sox2 interference was significantly higher than that of control cells,and the expression level of ABC drugresistant family genes decreased significantly after Sox2 interference.  Conclusion: After interfering with Sox2, the expression of Sox2 in SW620 cells was effectively inhibited. After interfering with Sox2, the sensitivity of SW620 cells to 5FU was significantly improved,and the expression level of ABC drugresistant genes was decreased, providing the possibility for seeking new gene targeted therapy.
2020 Vol. 30 (01): 7-11,17 [Abstract] ( 40 ) [HTML 1KB] [ PDF 2870KB] ( 543 )
12
Effect of lincRNA-p21 knockdown in macrophage on the biological function of colon cancer cells CT26
Effect of lincRNA-p21 knockdown in macrophage on the biological function of colon cancer cells CT26[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 12-17>')" href="#"> ZHOU Li-ning1,2， LIU Yue-qin3， SU Zhao-liang1， ZHOU Feng2， XU Hua-xi1
[Abstract\]Objective: To investigate the effect of differential expression of lincRNAp21 in macrophage on the tumor growth and tumorbearing mice, and provide a new target for the diagnosis and treatment of cancers. Methods: CT26 tumor cells were cultured and supernatant were collected to stimulate mouse macrophages RAW264.7 . The expression of IL-6 ,IL-4, IL-10, lincRNA-p21 were detected by RTqPCR. The expression of lincRNAp21 in macrophages transfected with si-lincRNA-p21 was detected by RT-qPCR. Co-cultured with CT26 after the macrophages transfected with NC and silincRNAp21, the apoptosis of CT26 was detected by flow cytometry, and the migration ability of CT26 was detected by transwell and wound healing assay. CT26 colon cancer tumor-bearing mice model were established, macrophages transfected with NC and si-lincRNA-p21 were injected to measure the volume and weight of tumor in tumor-bearing mice. Results: The expression of IL-6 was down-regulated (P<0.01) but the expression of IL4 and IL10 were upregulated (P<0.01) compared with the normal RAW264.7. LincRNA-p21 expression in RAW264.7 was higher than that in normal RAW264.7 (P<0.01). Compared with the NC group, the expression of lincRNA-p21 in macrophages transfected with si-lincRNA-p21 was down-regulated (P<0.01). Co-cultured with CT26 after the macrophages transfected with NC and si-lincRNA-p21, the expression of IL-6 was up-regulated (P<0.01) but the expression of IL4 and IL10 were downregulated (P<0.01). Compared with the NC group, the apoptosis of CT26 cells co-cultured with the macrophages transfected with si-lincRNAp21 group were increased (P<0.01), and the migration ability decreased (P<0.05). Compared with the NC group, the tumor volume and weight of tumor in tumorbearing mice injected with silincRNA-p21 macrophages were decreased (P<0.05) . Conclusion: The knockdown of lincRNA-p21 in macrophage can promote the increase of apoptosis of CT26 and inhibit its ability of migration and delay the growth of tumor in tumorbearing mice.
2020 Vol. 30 (01): 12-17 [Abstract] ( 23 ) [HTML 1KB] [ PDF 4723KB] ( 579 )
18
CXCL12-CXCR4 axis promotes proneural-mesenchymal transition in glioblastoma
CXCL12-CXCR4 axis promotes proneural-mesenchymal transition in glioblastoma
[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 18-23>')" href="#">		 XU Xue-wen1, Song Lian1, YANG Xiao-xiao1, SHI Hui1,GONG Ai-hua2, WANG Ming1, ZHANG Li-rong1
[Abstract]Objective: To investigate the effect of CXC chemokine ligand 12CXC chemokine receptor 4 (CXCL12CXCR4) axis on proneuralmesenchymal transition in glioblastoma. Methods: The difference of CXCR4 mRNA in glioma molecular subtyping tumor tissues and its effect on tumor patients′ survival rate were analyzed based on data in the TCGAGBM gene database; human glioma LN428 and U87MG cells were pretreated with different concentrations of CXCL12(0, 20, 40, 60, 80, 100 ng/mL) and 20 μmol/L plerixafor (selective CXCR4 antagonist) for 48 h, the expression levels of OLIG2, Ecadherin, YKL40, Ncadherin and Vimentin were detected by Western blotting, the cell migration and invasion ability were detected by Transwell assay, the proliferation ability was detected by CCK8 assay and colony formation assay. Results: According to the data in the TCGAGBM database, compared with normal group, the mRNA expressions of CXCR4 in glioma patients was significantly increased, and it was negatively correlated with the survival rate of patients. The mRNA expression level of CXCR4 in mesenchymal glioblastoma tissue samples was significantly higher than that in proneural glioblastoma (all P<0.05). Compared with control group, the expressions of mesenchymalassociated proteins YKL40, Ncadherin and Vimentin were significantly increased in CXCL12 group, while the expressions of the proneuralrelated proteins OLIG2, Ecadherin were markedly decreased, migration and invasion ability and proliferative ability of glioma LN428 and U87MG cells were greatly enhanced (all P<0.05). After treatment with plerixafor, migration and invasion ability and proliferative ability of glioma LN428 and U87MG cells were remarkably inhibited, expression of mesenchymalrelated proteins was highly decreased, and expression of proneuralrelated proteins was increased (all P<0.05). Conclusion: The CXCL12CXCR4 axis promotes proneuralmesenchymal transition, migration, invasion and proliferation of glioblastoma.
2020 Vol. 30 (01): 18-23 [Abstract] ( 38 ) [HTML 1KB] [ PDF 5526KB] ( 583 )
24
The influence of cGAS gene silencing on proliferation, migration and apoptosis of esophageal squamous cell carcinoma TE-1 cells
The influence of cGAS gene silencing on proliferation, migration and apoptosis of esophageal squamous cell carcinoma TE-1 cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 24-28>')" href="#"> WANG Yun-fan1, DAI Dong-fang2，*, FU Cong1, CHEN De-yu2

Objective: To investigate the expression of cyclic guanosinemonophosphate adenosine monophosphate synthase(cGAS) protein in esophageal squamous cell carcinoma，and to clarify the influence of cGAS gene silencing on proliferation, migration and apoptosis of esophageal squamous carcinoma cell line TE1. Methods: Sixty cases of esophageal cancer and adjacent tissues were examined by immunohistochemiscal staining. The expression of cGAS in the normal esophageal epithelial cell and esophageal squamous cell was detected by fluorescence quantitative PCR and Western blotting. Lentivirus infection was used to construct stable shcGAS cell lines TE-1. qRT-PCR and Western blotting were used to assess cGAS knockdown efficiency. The proliferation ability of cells was detected by CCK8 assay and clone formation assay. The migration ability of cells was detected by cell scratch test and Transwell test. The apoptosis of cells was detected by flow cytometry. Results: Immunohistochemical evaluation results showed that the expression of cGAS in human ESCC was significantly higher than that of matched adjacent tissues. The expression levels of cGAS was low in normal esophageal epithelial cells Het1A and high in esophageal squamous cell lines TE-1,KYSE-150. The cGAS expression levels of sh-cGAS groups was significantly lower than that in control group by RTPCR and Western blotting. Compared with the control group, CCK8 assay, Colony formation assay, cell scratch test and Transwell test showed that the proliferation and migration abilities of shcGAS groups had significant reductions, whereas flow cytometry showed that the apoptosis of shcGAS groups was significant stronger. Conclusion: Knockdown of cGAS gene can inhibit the proliferation ability and migration ability, and effectively promote apoptosis.
2020 Vol. 30 (01): 24-28 [Abstract] ( 34 ) [HTML 1KB] [ PDF 3670KB] ( 567 )
29  Construction of a stable LncRNA458314 knockout renal cancer cell line using the CRISPR/Cpf1 system and its function evaluation
 Construction of a stable LncRNA458314 knockout renal cancer cell line using the CRISPR/Cpf1 system and its function evaluation[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 29-33>')" href="#"> ZHOU Yang, CHEN Bing-hai, WANG Cheng-yue
[Abstract]Objective: A new gene editing system, CRISPR/Cpf1, was used to stably knock out LncRNA458314 in renal cell carcinoma 769P, and to preliminarily study the role of LncRNA458314 in renal cancer. Methods: LncRNA458314 of renal cell carcinoma 769P was knocked out using the CRISPR/Cpf1 system, and the knockout efficiency was evaluated by fluorescent quantitative PCR. CCK8 assay was used to detect the proliferation of 769P cells after LncRNA458314 knockout; Western blotting was used to detect the expression of pAKT, pERK and LC3A/B proteins in 769P cells after LncRNA458314 knockout. Results: The quantitative PCR results showed that the CRISPR/Cpf1 system successfully knocked out the target LncRNA458314, and after multiple fluorescent quantitative PCR detection and comparison, two 769P/LncRNA458314 monoclonal cells 6 # and 13 # with stable low expression of LncRNA458314 were selected for subsequent experiment. CCK8 experiments showed that after LncRNA458314 was knocked out, the proliferative capacity of renal cancer 769P cells was significantly reduced; Western blotting results showed that the expression levels of pAKT and pERK in 769P cells after knockout of LncRNA458314 were significantly reduced, and LC3A/B expression levels were significantly increased. Conclusion: LncRNA458314 may exert its biological functions by regulating the expression of pAKT, pERK and LC3A/B.
2020 Vol. 30 (01): 29-33 [Abstract] ( 60 ) [HTML 1KB] [ PDF 1212KB] ( 569 )
34
Dynamic changes of regulatory T cells in the process of radiation-induced pulmonary fibrosis in mice
Dynamic changes of regulatory T cells in the process of radiation-induced pulmonary fibrosis in mice[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 34-38>')" href="#"> WANG Cai-hong1,2, PAN Xiao-xian1,2, CHEN Jin-mei2,3,4, HUANG Fei3,4,5, WU Jian-dong2, HONG Jin-sheng2,3,4
[Abstract]Objective: To investigate the dynamic changes of regulatory T cells(Treg cells) in the progress of radiationinduced pulmonary fibrosis. Methods: Eighty eightweekold female C57BL/6 mice were randomly divided into control group and irradiation group, forty mice in each group. Mice thoraxes were irradiated with a single dose of 15 Gy 6 MV Xray delivered by a liner accelerator to establish the mice model of radiationinduced pulmonary fibrosis. The peripheral blood, lungs and spleens of mice were separated on Day 2, Day 17 and Month 3, Month 5 after irradiation. Lungs in irradiation group and control group were subjected to paraffin section, HE staining and Masson staining were performed to analyze pathological changes. The proportion of Treg cells in CD4+T cells in peripheral blood, lungs and spleens was detected by flow cytometry at each time; Using 2×4 factorial design analysis of variance, we detected the differences in the proportion of Treg cells at different time points after irradiation with or without irradiation. Results: The inflammatory pathological characteristics in lungs of irradiation group were observed on the Day 2 and Day 17 after irradiation; on the Month 5 after irradiation, a large amount of collagen deposition was observed in the alveolar space of the irradiated group, the collagen volume fraction in lungs of irradiation group was significantly higher than that of control group(P＜0.01). On the Day 2 and Day 17 after irradiation, the proportion of Treg cells in the peripheral blood, lungs and spleens of irradiated group was significantly higher than that of control group(P＜0.01); on the Month 3 after irradiation, the proportion of Treg cells in peripheral blood, lungs and spleen of irradiation group gradually decreased（P<0.01）; on the Month 5 after irradiation, the proportion of Treg cells in peripheral blood, lungs of irradiated group decreased to the level of control group, while decreased to less than control group in spleen（P<0.01）. There was no significance difference between the control group at each time(P＞0.05). Conclusion: Treg cells showed dynamic pattern change in the progress of irradiationinduced pulmonary fibrosis in mice, and the overall presented a trend of increasing first and then decreasing.
2020 Vol. 30 (01): 34-38 [Abstract] ( 49 ) [HTML 1KB] [ PDF 31976KB] ( 481 )
39
Bioinformatics analysis and function of renal cell carcinoma based on multiple databases
Bioinformatics analysis and function of renal cell carcinoma based on multiple databases[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 39-44>')" href="#"> ZHANG Jin-hu, ZHOU Yang, WANG Cheng-yue, ZOU Yuan-zhang, WANG Hao-yang, LU Qiu, SUN Hao
[Abstract]Objective: To explore the difference in gene expression between renal cancer metastatic tumor cells and primary renal cancer cells by bioinformatics technology, and to find the key differential genes and the potential molecular mechanism. Methods: The gene chip data GSE85258 of human renal cancer metastases and primary renal cancer was downloaded from the GEO gene chip database, and the differentially expressed genes of renal cancer metastases and primary renal cancer were analyzed using the GCBI online tool. By analyzing the differentially expressed genes GO and Pathway, the differential genes that meet both GO and Pathway were screened out; and then we constructed a gene network map of the interactions and coexpression relationships between the differential genes; and constructed a pathway network map of the differential genes. The function of differential genes in renal cancer cells was verified by in vitro cell invasion experiments. Results: A total of 3 000 differential genes were screened in the GSE85258 data set, of which SFTPA2 increased significantly and SLC17A3 decreased markedly. GO analysis found that these differential genes mainly focused on multiple biological functions, such as DNAdependent transcription, signal transduction, and small molecule metabolic processes. Pathway analysis showed that these differential genes are mainly involved in biological processes, such as endoplasmic reticulum protein processing, metabolic pathways, and cancerrelated pathways. Analysis of differential gene interactions revealed that MAPK1 is the core signalling intermediary, and PRKCA, NRAS, and NOS1 genes directly interact with MAPK1. The coexpression analysis of differential genes showed that there were positive and negative correlations between the coexpressions of the differential genes. Among them, the 10 genes most closely related to the surrounding genes were positively correlated with each other. Pathway network analysis shows that the MAPK signal pathway is the most upstream and downstream pathway, including cell cycle, apoptosis, P53 signal pathway, cancerrelated pathway and other signal pathways related to tumorigenesis. Invasion experiments showed that inhibition of SFTPA2 expression could reduce the invasion ability of renal cancer cells (t=18.44, P<0.01). Conclusion: Differentially expressed genes are found in metastatic tumor cells and primary renal cancer cells through bioinformatics techniques. Among them, SFTPA2, MAPK1, PRKCA, and NOS1 may be key differential genes and may play roles in MAPK signaling pathways.
2020 Vol. 30 (01): 39-44 [Abstract] ( 43 ) [HTML 1KB] [ PDF 1217KB] ( 561 )
45 Effect of sulfur fumigation on volatile oils components in chrysanthemum
[Abstract]Objective: To explore the differences of volatile oil components in Chrysanthemum before and after sulfur fumigation. Methods: The volatile oils of chrysanthemum before and after sulfur fumigation were extracted by steam distillation. The components of the volatile oils before and after sulfur fumigation was detected by GCMS/MS. Results: A total of 201 peaks were detected in the volatile oils of chrysanthemum before sulfur fumigation, 227 in the volatile oils of chrysanthemum after sulfur fumigation. There were 27 components in the volatile oils of chrysanthemum before and after sulfur fumigation, 72 components appeared after sulfur fumigation and 27 components disappeared.  Conclusion: The quantity, components and content of volatile oils in Chrysanthemum changed greatly before and after sulfur fumigation, which provided data for the identification and quality control of chrysanthemum and other sulfur fumigated traditional Chinese medicine.
2020 Vol. 30 (01): 45-52 [Abstract] ( 46 ) [HTML 1KB] [ PDF 1062KB] ( 569 )
53  Effect of Hsa_circ_0001947 on proliferation and apoptosis of gastric cancer cells#br#
 Effect of Hsa_circ_0001947 on proliferation and apoptosis of gastric cancer cells#br# [J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 53-57>')" href="#"> CHEN Zheng-wei1, ZHANG An-wei2, ZHANG Yao1, ZHANG Xuan-feng1, YUAN Hai-tao1, BU Xue-feng3
[Abstract]Objective: To investigate the expression of circular RNA hsa_circ_0001947(circ_0001947) in gastric cancer cells and tissues and its effect on proliferation and apoptosis of gastric cancer cells. Methods: Five kinds of circular RNA, differentially expressed in 5 cases of gastric carcinoma and paracancerous tissues, were screened by high throughput sequencing. The most obvious circ_0001947 was selected for subsequent study. Realtime fluorescence quantitative reverse transcription PCR was used to detect the expression of circ_0001947 in 75 cases of clinical gastric carcinoma, paracancerous tissues, human gastric cancer BGC-823, HGC-27, MGC-803, SGC-7901 cell lines and normal gastric epithelial GES-1 cell line. The cell lines BGC823 and SGC7901 were divided into two groups：si-circ_0001947 group(transfected with si-RNA) and si-NC group (transfected with irrelevant sequence). Cell viability was determined by CCK8 assay after 0, 12, 24, 48, 72 h of cell culture. Cloning test was used to determine cell proliferation; the cell apoptosis was determined by flow cytometry, Western blotting was used to determine the expression levels of apoptosis-related proteins Cleaved-caspase-3, Bcl-2 and bax. Results: The differentially expressed cyclic RNA in gastric adenocarcinoma tissues screened by high throughput sequencing were hsa_circ_0000033, hsa_circ_0000038, hsa_circ_0004916, hsa_circ_0058092 and hsa_circ_0001947. The expression of circ_0001947 in gastric carcinoma was significantly higher than that in paracancerous tissue (P<0.01). The expression of circ_0001947 in human gastric cancer cell lines BGC-823, HGC-27, MGC-803, SGC-7901 was significantly higher than that in normal gastric epithelial GES1 cell(P<0.05). si-RNA could significantly inhibit the expression of circ_0001947 in BGC-823 and SGC-7901 cells. The absorbance of the BGC823 and SGC7901 cells in the si-circ_0001947 group was significantly lower than that of the si-NC group (P<0.01). The clone formation rate of BGC823 and SGC7901 cells in si-circ_0001947 group was remarkably lower than that in si-NC group (P<0.01). The results of flow cytometry showed that the apoptosis rate of BGC823 and SGC-7901 cells in si-circ_0001947 group was remarkably higher than that in si-NC group(P<0.01). Compared with siNC group, the expression of Cleaved-caspase-3and Bax in BGC-823, SGC-7901 cells of si-circ_0001947 group was significantly upregulated, while the expression of Bcl2 was significantly down-regulated(P<0.05). Conclusion: circ_0001947 was highly expressed in human gastric cancer cells and tissues. Knockdown of circ_0001947 expression could inhibit the proliferation and induce apoptosis of gastric cancer cells.
2020 Vol. 30 (01): 53-57 [Abstract] ( 46 ) [HTML 1KB] [ PDF 2909KB] ( 665 )
58  Expression of non-coding RNA ArpH in Salmonella enterica serovar
Typhi in the different growth stages and under different stress conditions
 Expression of non-coding RNA ArpH in Salmonella enterica serovar
Typhi in the different growth stages and under different stress conditions
[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 58-62>')" href="#">		 XIONG Chang-yan, LI Xue-jiao, HUANG Xin-xiang
[Abstract]Objective: To investigate the expression characteristics of ArpH under different environmental stress conditions and the effects of two component regulatory system genes and oxygen stress regulator gene on the expression of ArpH in Salmonella enterica serovar Typhi （S. Typhi）. Methods: The ArpH expression of S. Typhi in different growth stages were analyzed by Northern blotting and qRT-PCR. qRT-PCR was used to analyze ArpH expression in S. Typhi under different stress conditions, including acidic, oxidative, hyperosmotic and heat stress. Effects of two component regulation system genes (ompR, rcsB) and oxygen stress regulator gene (oxyR) on the expression of ArpH in S. Typhi were analyzed by qRT-PCR. Results: The expression of ArpH was the highest in S. Typhi at the late logarithmic phase. The expression of ArpH increased gradually from the early logarithmic phase to the stable phase, and decreased after the stable phase. The expression level of ArpH decreased significantly under acidic stress and increased under oxidative stress, while the expression of ArpH was not affected by hyperosmotic stress and heat stress. When ompR, rcsB and oxyR genes were deleted separately, the expression of ArpH decreased significantly. Conclusion: The ArpH was beneficial to bacterial growth in the acid and oxidative stress，the genes ompR, rcsB and oxyR of S. Typhi had a positive regulatory effect on ArpH.
2020 Vol. 30 (01): 58-62 [Abstract] ( 33 ) [HTML 1KB] [ PDF 935KB] ( 553 )
63
Expression of FLCN in cervical squamous cancer tissue and its effect on proliferation and apoptosis in SiHa cells
Expression of FLCN in cervical squamous cancer tissue and its effect on proliferation and apoptosis in SiHa cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 63-67>')" href="#"> ZHANG Yu, YANG Pei-fang, CHEN Xia, LUAN Xiao-jin, YAN Yi-dan, YU Jun
[Abstract]Objective: To investigate the expression of folliculin(FLCN) in cervical squamous cancer tissue and its effect on the cell proliferation and apoptosis in cervical carcinoma. Methods: The mRNA and protein expressions of FLCN in cervical squamous cancer tissue were detected by qRTPCR and immunohistochemical technique. After overexpression or knocking down of FLCN in cervical squamous cancer SiHa cells, the FLCN mRNA expression was detected by qRT-PCR, the cell proliferation was evaluated by CCK8 -and phosphorylated histone H3 (PH3) staining, the cell apoptosis was measured by TUNEL. Results: Compared with the control group, FLCN mRNA and protein level expression in cervical squamous cancer groups were significantly reduced (P<0.05). After FLCN siRNA was transfected, cell proliferation increased and apoptosis decreased, PH3 protein positive ratio increased, TUNEL positive cell ratio decreased (P<0.05 or <0.01 ). Afer overexpression of FLCN, cell proliferation was inhibited and apoptosis was enhanced, the proportion of PH3 proteinpositive cells decreased, and the proportion of TUNELp-ositive cells increased(all P<0.01). Conclusion: FLCN gene was lowexpression in cervical squamous cancer tissue, which could inhibit proliferation and promote apoptosis of SiHa cells.
2020 Vol. 30 (01): 63-67 [Abstract] ( 50 ) [HTML 1KB] [ PDF 3791KB] ( 550 )
68
Protective effects of PCV-VG combined with PEEP on lungs in patients with lung carcinoma undergoing thoracoscopic radical resection
Protective effects of PCV-VG combined with PEEP on lungs in patients with lung carcinoma undergoing thoracoscopic radical resection[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 68-71>')" href="#"> GAO Rong, BIAN Qing-ming
[Abstract]Objective: To evaluate the effects of pressure controlled ventilationvolume guaranteed (PCVVG) combined with positive endexpiratory pressure (PEEP) on hemodynamics, respiratory mechanics, and oxygenation in patients with pulmonary carcinoma undergoing thoracoscopic radical resection. Methods: Forty patients with lung cancer undwent thoracoscopic lung radical resection were randomly divided into 2 groups: in routine group, both two lung ventilation and one lung ventilation were performed, patients received the ventilation protocol consisting of 8 mL/kg VT, 0 cmH2O PEEP under volume controlled ventilation (VCV), while in combination group, patients received 8 mL/kg VT, PEEP 5 cmH2O under PCVVG. Invasive arterial systolic blood pressure(ASBP) and diastolic blood pressure(ADBP) and heart rate were monitored and recorded at baseline(T0), at the time just before one lung ventilation (T1), at 30 min (T2), 60 min (T3), 120 min after one lung ventilation (T4) and 30 min after the restoration of two lung ventilation (T5). PPeak, PPlat, Cdyn were monitored and recorded at the selected time points, arterial blood samples were collected at T1-T5 time points for blood gases analyse and oxygenation index was calculated. Results: The patterns of hemodynamic parameters were similar between the two groups(P>0.05). Compared with routine group, PPeak, PPlat in combination group were significantly decreased at T2-T5 (P<0.05), while Cdyn, PaO2 and oxygenation index at T1-T5 were significantly higher(P<0.05). Conclusion: PCVVG combined with PEEP could provide stable hemodynamics, improve respiratory mechanics and further increase oxygenation for patients with lung carcinoma undergoing thoracoscopic radical resection.
2020 Vol. 30 (01): 68-71 [Abstract] ( 50 ) [HTML 1KB] [ PDF 1454KB] ( 542 )
72  Predictive value of serum interleukin-33 levels in the patients
with atherosclerotic cerebral infarction
 Predictive value of serum interleukin-33 levels in the patients
with atherosclerotic cerebral infarction
[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(01): 72-74>')" href="#">		 WU Zheng-fu1, HUANG Fei-ran2, XU Yu-hao2, YU Ming2
[Abstract]Objective: To explore the prognostic value of serum IL33 in the prediction of the patients with atherosclerotic cerebral infarction (ACI). Methods: A total of 142 patients with ACI were enrolled. Their clinical data and National Institutes of Health Stroke Scale (NIHSS) were collected, and the serum IL33 level was detected by ELISA before treatment. Modified Rankin Scale (mRS) score was performed 90 days, and the patients were divided into the good prognosis group (n=69) and the poor prognosis group (n=73) according to mRS score. The general baseline data and serological data of the patients in the two groups were compared, respectively. The Spearman correlation between NIHSS score and IL33 level was analyzed, and Logistic regression was used to analyze the factors which influence the prognosis of the patients with ACI and the ROC curve of serum IL33 level was further plotted. Results: Age, past history of cerebral infarction and NIHSS score in poor prognosis group were higher than those in good prognosis group (P<0.05), and the serum IL33 level was significantly lower than those in good prognosis group (P<0.01). IL33 level was negatively correlated with the NIHSS score at admission(r=-0.581, P<0.01). Higher NIHSS score at admission was a risk factor for poor prognosis (P<0.01), while higher serum IL33 level was a protective factor (P<0.01). The ROC curve showed that the area under the curve was 0.835, the sensitivity was 91.3%, and the specificity was 72.5%. Conclusion: The decreased serum IL33 levels could be used as a predictor of poor prognosis for patients with ACI.
2020 Vol. 30 (01): 72-74 [Abstract] ( 53 ) [HTML 1KB] [ PDF 822KB] ( 614 )
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2020 Vol. 30 (01): 76- [Abstract] ( 32 ) [HTML 1KB] [ PDF 1117KB] ( 841 )
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2020 Vol. 30 (01): 80-86 [Abstract] ( 45 ) [HTML 1KB] [ PDF 872KB] ( 613 )
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2020 Vol. 30 (01): 86-92 [Abstract] ( 44 ) [HTML 1KB] [ PDF 991KB] ( 1186 )
江苏大学学报:医学版
　

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