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Abstract
Objective: To investigate the expression of cyclic guanosinemonophosphate adenosine monophosphate synthase(cGAS) protein in esophageal squamous cell carcinoma，and to clarify the influence of cGAS gene silencing on proliferation, migration and apoptosis of esophageal squamous carcinoma cell line TE1. Methods: Sixty cases of esophageal cancer and adjacent tissues were examined by immunohistochemiscal staining. The expression of cGAS in the normal esophageal epithelial cell and esophageal squamous cell was detected by fluorescence quantitative PCR and Western blotting. Lentivirus infection was used to construct stable shcGAS cell lines TE-1. qRT-PCR and Western blotting were used to assess cGAS knockdown efficiency. The proliferation ability of cells was detected by CCK8 assay and clone formation assay. The migration ability of cells was detected by cell scratch test and Transwell test. The apoptosis of cells was detected by flow cytometry. Results: Immunohistochemical evaluation results showed that the expression of cGAS in human ESCC was significantly higher than that of matched adjacent tissues. The expression levels of cGAS was low in normal esophageal epithelial cells Het1A and high in esophageal squamous cell lines TE-1,KYSE-150. The cGAS expression levels of sh-cGAS groups was significantly lower than that in control group by RTPCR and Western blotting. Compared with the control group, CCK8 assay, Colony formation assay, cell scratch test and Transwell test showed that the proliferation and migration abilities of shcGAS groups had significant reductions, whereas flow cytometry showed that the apoptosis of shcGAS groups was significant stronger. Conclusion: Knockdown of cGAS gene can inhibit the proliferation ability and migration ability, and effectively promote apoptosis.

Received: 17 December 2019

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	Articles by authors
	WANG Yun-fan1
	DAI Dong-fang2
	*
	FU Cong1
	CHEN De-yu2

Cite this article:

WANG Yun-fan1,DAI Dong-fang2,*等.

The influence of cGAS gene silencing on proliferation, migration and apoptosis of esophageal squamous cell carcinoma TE-1 cells

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(01): 24-28.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I01/24

［1］Sun L, Wu J, Du F, et al. Cyclic GMPAMP synthase is a cytosolic DNA sensor that activates the type Ⅰ interferon pathway\[J\].Science,2013,339(6121):786-791.

［2］Kato K, Ishii R, Goto E, et al. Structural and functional analyses of DNAsensing and immune activation by human cGAS\[J\]. PLoS One,2013,8(10):e76983.

［3］Li XD, Wu J, Gao D, et al. Pivotal roles of cGAScGAMP signaling in antiviral defense and immune adjuvant effects\[J\]. Science,2013,341（6152）:1390-1394.

［4］Liu H, Zhang H, Wu X, et al. Nuclear cGAS suppresses DNA repair and promotes tumorigenesis\[J\].Nature,2018,563(7729):131-136.

［5］Gray EE, Treuting PM, Woodward JJ, et al. Cutting edge: cGAS is required for lethal autoimmune disease in the Trex1deficient mouse model of AicardiGoutières syndrome\[J\].J Immunol,2015,195(5):1939-1943.

［6］赫捷,邵康. 中国食管癌流行病学现状、诊疗现状及未来对策\[J\].中国癌症杂志, 2011,21(7): 501-504.

［7］Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries\[J\]. CA Cancer J Clin,2018,68(6):394-424.

［8］Pennathur A, Gibson MK, Jobe BA, et al. Oesophageal carcinoma\[J\].Lancet,2013,381(9864)： 400-412.

［9］Wu J, Sun L, Chen X, et al. Cyclic GMPAMP is an endogenous second messenger in innate immune signaling by cytosolic DNA\[J\]. Science, 2013, 339(6121):826-830.

［10］Lhuillier C, Rudqvist NP, Elemento O, et al. Radiation therapy and antitumor immunity: exposing immunogenic mutations to the immune system\[J\].Genome Med,2019,11(1):40.

［11］Sato H, Okonogi N, Yoshimoto Y, et al. Radiotherapy and PDL1 expression\[J\].Gan To Kagaku Ryoho,2019,46(5):845-849.

［12］Otto G. Inhibition by nuclear cGAS\[J\]. Nat Rev Mol Cell Biol, 2018,19 (12): 752-753.

［13］Harding SM, Benci JL, Irianto J, et al. Mitotic progression following DNA damage enables pattern recognition within micronuclei\[J\]. Nature,2017, 548(7668):466-470.

［14］Mackenzie KJ, Carroll P, Martin CA, et al. cGAS surveillance of micronuclei links genome instability to innate immunity\[J\]. Nature, 2017, 548(7668): 461-465.