Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2013 Vol.23 Issue.5 Published 2013-09-30

Article

	369	Construction and identification of cag1 gene mutant of Helicobacter pylori
		LING Feng1,2， WANG Xia-chun1， CHEN Cheng1， YU Min1， ZHU Hong1， LUO Cai-feng3， SHAO Shi-he1
		[Abstract]Objective: To establish cag1 gene mutant of Helicobacter pylori(H.pylori) NCTC11637 for exploring the influence of cag1 gene on cytotoxin associated gene pathogenicity island(cag PAI). Methods: The cag1 gene deletion fragment(upstream and downstream of the open reading frames) was amplified by two pairs of primers designed according to the sequence of cag1 and its upper and down streams. Then electroporation was used to transfect the suicide plasmid into H.pylori. Positive clones were screened out on the basis of kanamycin resistance and confirmed by PCR and DNA sequencing. GES-1 cells were infected with either the obtained cag1 gene mutant or the wild-type strain for 8 h. Morphological changes of infected cells were observed under microscope. Results: We generated and identified a cag1 gene mutant of H.pylori. The cag1 gene mutant caused much less scattering and elongation of GES-1 cells than the H.pylori wild-type strain. Conclusion: The cag 1 gene mutant of H.pylori was successfully obtained, which proved that the absence of cag1 strongly affected the virulence of H.pylori.
		2013 Vol. 23 (5): 369-372,376 [Abstract] ( 1451 ) [HTML 1KB] [ PDF 1142KB] ( 1836 )

	373	Puerarin protection against cerebral ischemia via inhibiting nNOS in rats
		WANG Wei-wei
		[Abstract]Objective: To evaluate the neuroprotection of puerarin against cerebral ischemia in SD rat models, and investigate the underlying mechanism. Methods: Fifty rats were randomly assigned into the following groups: shamoperated group, ischemia/reperfusion group, vehicletreated group and drugtreated group. Transient brain ischemia (15 min) and reperfusion was induced by the fourvessel occlusion method (4VO). The expression and activity of neuronal NO synthase （nNOS） and the survival of neurons were performed by Western blotting, cresyl violet staining and TUNEL methods. Results: The drugtreated group showed high levels of neurons, which was consistent with the TUNEL results, and higher levels of nNOS phosphorylation, compared with the ischemia/reperfusion group (both P<0.05). Conclusions: Puerarin could exert its neuroprotection from cerebral ischemia by increasing nNOS (Ser847) phosphorylation.
		2013 Vol. 23 (5): 373-376 [Abstract] ( 1023 ) [HTML 1KB] [ PDF 1020KB] ( 1561 )

	377	Effect of gene CTGF transfection on the expression of MMP-9 and VEGF and proliferation in human pancreatic cancer cells
		JIANG Jian-guo1, JIAO Xia2, ZHU Xiao-wei2
		Objective: To investigate the effect of connective tissue growth factor (CTGF) on the proliferation in human pancreatic cancer cell line SW1990, with a focus on the expression of matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor (VEGF). This study was designed to explore the underlying mechanism for the role of CTGF in the development of pancreatic cancer. Methods: pcDNA3.0CTGF and pcDNA3.0 were transfected into SW1990 cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blotting were employed to identify mRNA and protein expression of CTGF in SW1990 cells, respectively. The expression of MMP-9 and VEGF in positive clones was detected by RT-PCR and Western blotting.Cell viability was assessed by dimethylthiazol diphenyl tetrazoliumbromide (MTT) method. Results: Positive clones C-5 and C-28 with CTGF over-expression were successfully established. Compared with non- transfected control group, the expressions of MMP-9 and VEGF in C-5 and C-28 was increased significantly, and the proliferation level of C-5 and C-28 was increased significantly. Conclusions: CTGF transfection could effectively enhance the expression of MMP-9 and VEGF and the proliferation in SW1990 cells which suggested a potential role for CTGF in gene therapy of pancreatic cancer.
		2013 Vol. 23 (5): 377-381 [Abstract] ( 1240 ) [HTML 1KB] [ PDF 1005KB] ( 1496 )

	382	Culture and identification of mouse bone marrow-derived endothelial progenitor cells in vitro
		DANG Sheng-chun1, CHEN Ji-fang2, FENG Shu2, LIU Bin2, WANG Ping-jiang2, ZHANG Jian-xin1
		[Abstract]Objective: To develope methods of in vitro culture, differentiation and identification of mouse bone marrow derived-endothelial progenitor cells (EPCs)．Methods： Collected EPCs from ICR mouse, differentiated mononuclear cells by density gradient centrifugation，cultured in medium with vascular endothelial growth factor(VEGF), fibroblast growth factor(FGF) and detected the cell surface markers of endothelial cells after 5 days,10 days,15 days,20 days. Results： Cells began to appear epithelial cell morphology after 5 days, and pseudopods appeared;after 10 days of culture, cells began to proliferate, turned round and spindle cells began to appear;after 15 days,cells showed typical morphology of endothelial cells, formed cobblestone like structure，after 20 days,cells performed like long fusiform pulling nets, just similar with mature endothelial cell morphology. Immunofluorescence stain and flow quantitative test results showed, CD34 expression level in EPCs with the extension of incubation time had a gradual weakening trend; VEGFR-2 expression in EPCs with the incubation time had a gradual increasing trend. Conclusions： EPCs from bone marrow cultured in specific conditions could be induced differentiate typical of mature ECs;it was convenient and had strong amplification ability.
		2013 Vol. 23 (5): 382-384,388 [Abstract] ( 1212 ) [HTML 1KB] [ PDF 1682KB] ( 2068 )

	385	Effect of middle layer by liquidphase deposition technology on bonding strength of porcelain and pure titanium
		JIANG Tao1, CHEN Wei-jun2, FENG Ge1, XUAN Zhi-gang1, WU Zhanao1, GAO Yu1
		[Abstract]Objective: To investigate the effect of middle layer with liquidphase deposition（LPD） technology on bonding strength of titanium and porcelain in different condition. Methods： Fifty pure titanium specimens were randomly divided into ten groups（n=5）according to different surface treatments after sandblasting. The surface of titanium was investigated by scanning electron microscope(SEM）.After the middle area of the samples was veneered with porcelain, a threepointflexuretest was used to evaluate the bonding strength of titanium to porcelain.Results： The bonding strength of groups of liquidphase deposition were significantly higher than control group(P＜0.05). The mean bonding strength of pH=9,0.3 mol/L silicate solution group was the highest\[(38.86±2.63)MPa\], it was significantly higher than those of other nine groups(P＜0.05). Conclusion： Middle layer prepared by LPD technology was significant in improving bonding strength of porcelain and pure titanium, the bonding strength was the highest by pH=9,0.3 mol/L silicate solution.
		2013 Vol. 23 (5): 385-388 [Abstract] ( 943 ) [HTML 1KB] [ PDF 1155KB] ( 1412 )

	389	Inhibition of arsenic trioxide on Hedgehog pathway in acute promyelocytic leukemia cell line
		YANG Dong-guang, CAO Feng-lin, YE Xiang-mei, ZHAO Hui, LI Yang, WANG Hong-ling, ZHOU Jin
		Objective: Human acute promyelocytic leukemia (APL)cell line NB4 was used as a model to discuss whether arsenic trioxide (ATO) can inhibit the expression of Hedgehog signaling pathway molecules，so that we can provide a theoretical basis for the application of ATO in other cancers with an aberrant activation of Hedgehog signaling pathway. Methods： Treated with ATO of different concentration, the expression of Hh pathway components such as Smo,Ptch,Gli1 and Gli2 were analyzed with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western Blot in NB4 at mRNA and protein level.Results： After ATO treatment, the expression of Smo, Ptch and Gli2 were significantly downregulated in NB4. Conclusion: ATO may inhibit the expression of Hh signaling pathway through the mRNA and protein inhibition of the key Gli2 target transcription factor and ATO may inhibit APL partly through the inhibition of Hedgehog signaling pathway.
		2013 Vol. 23 (5): 389-392 [Abstract] ( 1421 ) [HTML 1KB] [ PDF 1173KB] ( 1594 )

	393	Effect of PECAM-1 siRNA on cell proliferation and apoptosis of irradiation-induced drug resistance CNE1/R cells of human nasopharyngeal carcinoma
		WEI Ji-lou1, LIU Fang2, JIANG Zhong-xiu3, CHENG Hong-lei2, LUO Su-mei2，WANG Yue-li2, WANG Ruo-yu2
		[Abstract]Objective: To investigate the effect of silencing platelet-endothelial cell adhesion molecule-1(PECAM-1) by small interfering RNA (siRNA) on cell proliferation and apoptosis of irradiation-induced drug resistance CNE1/R cells of human nasopharyngeal carcinoma. Methods: The CNE1/R-specified siRNA was designed and synthesized. The purity of the transfected cells were screened out by G418. The expression of PECAM-1 was detected by western blotting. Cell growth and apotosis was assayed by MTT and flow cytometry, respectively. Results: The expression of PECAM-1 protein in CNE1/R cells was significantly downregulated. Compared with negative group and control group, experiment group showed significantly decreased proliferation ability in CNE1/R cells, whereas cell apoptosis rate was increased. Conclusion: PECAM-1 siRNA could inhitit the growth and promote the apoptosis of CNE1/R cell, which suggested PECAM-1 might play a key role in CNE1/R cell growth and apoptosis.
		2013 Vol. 23 (5): 393-396,401 [Abstract] ( 1665 ) [HTML 1KB] [ PDF 2057KB] ( 1575 )

	397	Construction of psiCHECK-2eGFP-mLumin dual fluorescent protein reporter gene and expression in eukaryotic cells
		PAN Xiao-yuan, WU Wei-jiang, LIU Yun, ZHANG Miao-miao, LIU Xia,DU Feng-yi, XIA Sheng, SHAO Qi-xiang
		Objective: To construct a new dual fluorescent protein reporter gene plasmid, psiCHECK-2-eGFP-mLumin. Methods: The dual fluorescent protein reporters gene plasmid was constructed by inserting the PCR amplification products of enhanced green fluorescent protein (eGFP) and mLumin into psiCHECK-2 vector to replace the firefly luciferase and renilla luciferase, respectively. The sequence of eGFP and mLumin were confirmed by PCR, and blasted online of the National Center for Biotechnology Information. HEK293T cells were transfected with psiCHECK-2-eGFP-mLumin plasmid and the expression efficiency of the fluorescent proteins was valuated. Results: The dual fluorescent protein reporters gene plasmid, psiCHECK-2-eGFP-mLumin, was successfully constructed by inserting the correct sequence of eGFP and mLumin into the psiCHECK-2 plasmid. The psiCHECK-2-eGFP-mLumin successfully mediated the gene expression of eGFP and mLumin in HEK293T cells. Conclusion: The psiCHECK-2-enhanced green fluorescent protein-mLumin plasmid was constructed successfully and expressed well in HEK293T cells.
		2013 Vol. 23 (5): 397-401 [Abstract] ( 3929 ) [HTML 1KB] [ PDF 1686KB] ( 2208 )

	402	Effect of up-regulating the NDRG1 gene expression on lung adenocarcinoma cells proliferation and apoptosis
		JIAO Xia1, ZHU Xiao-wei1, LI Shun-long2, DAI Gui-hong1, JIANG Xiao-qin1,YAO Juan3, XIAO Wei1，YU Hong1
		Objective: To evaluate the effect and mechanism of Nmyc downstream-regulated gene 1 (NDRG1) on the proliferation and apoptosis of lung adenocarcinoma cells. Methods: pcDNA3.0-NDRG1 and pcDNA3.0 were transfected into A549 cells by lipofectamine; and positive clones were screened by G418. Quantitative real time polymerase chain reaction(qRT-PCR) and western blotting were used to identify mRNA and protein expressions of NDRG1 in A549 cells, respectively. The expressions of P21 and marine doubleminute 2(MDM2) in positive clones were detected by qRT-PCR and western blotting. The cell proliferation and apoptosis were observed by MTT assay and flow cytomertry. Results: Positive clone N11 with NDRG1 over-expression were successfully established. Compared with non-transfected control group, the expression of P21 and the apoptosis of N11 were significantly increased, however, the expression of MDM2 and the proliferation of N11 were significantly decreased. Conclusions: NDRG1 transfection could effectively promote the apoptosis and inhibit the proliferation of A549 cells by upregulating P21 expression and downregulating MDM2 expression in A549 cells.
		2013 Vol. 23 (5): 402-406 [Abstract] ( 1409 ) [HTML 1KB] [ PDF 1266KB] ( 1381 )

	407	Therapeutic effect of tendrilleaf fritillary bulb on acute respiratory distress syndrome rats
		YU Ya-jie1, LIU Chang2, ZHANG Yu3, HAN Dong2
		Objective: To observe the effect of tendrilleaf fritillary bulb on acute respiratory distress syndrome (ARDS) rats induced by oleic acid. Methods: The ARDS model was constructed by injection of oleic acid intravenously. Forty model rats were randomly divided into ARDS model group, lowdose tendrilleaf fritillary bulb group, highdose tendrilleaf fritillary bulb group and dexamethasone group. Another ten Wistar rats were selected as the control group. Left lung wet/dry weight (W/D) ratio, lung tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA) content were detected, serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) levels were assayed by enzyme linked immunosorbent assay.Results: Compared with the control group, the model group showed lower levels of lung wet/dry weight (W/D) ratio, MDA,TNF-α, IL-1β, IL-6, but with higher levels of SOD activity （P＜0.01 or P＜0.05）. The tendrilleaf fritillary bulb groups showed lower levels of lung wet/dry weight (W/D) ratio, MDA,TNF-α, IL-1β, IL-6, but higher levels of SOD, compared with the model group (both P<0.05). Conclusion: Tendrilleaf fritillary bulb could reduce lung tissue damage induced by oleic acid, and has the therapeutic effect on ARDS rat, which may be by preventing lipid peroxidation in the course of ARDS.
		2013 Vol. 23 (5): 407-410 [Abstract] ( 1284 ) [HTML 1KB] [ PDF 771KB] ( 1515 )

	411	Relationship between interleukins and microalbuminuria／creatinine ratio in elderly patients with type 2 diabetes mellitus
		LUO Hong-lin, JI Hong-ge, XU Chang-gen, ZHU Yang-quan
		Objective: To explore the interleukins level and its relationship with microalbuminuria／creatinine ratio(ACR) in elderly type 2 diabetes mellitus (T2DM). Methods: A total of 117 elderly patients with T2DM and 30 healthy controls participated in this study. Subjects with T2DM were divided into three groups using urinary albumin-to-creatinine ratio (ACR): a normoalbuminuric group (group NA, n=29, UAER<30 mg／24 h), a microalbuminuria group (group MA, n=48, UAER 30-300 mg／24 h) and a macroalbuminuria group (group ODN, n=40, UAER>300 mg／24 h). The serum interleukin-2 (IL-2)、IL-6、 IL-8、IL-10、IL-18 concentrations were determined by enzyme linked immunosorbent assay (ELISA), albuminuria／creatinine ratio (ACR) was detected by DCA2000. Results: Compared with the control group, the groups of T2DM had higher serum IL-2、IL-6 、IL-8 、IL-18 levels and lower serum IL-10 concentration(P<0.05 respectively). Group ODN had higher serum concentrations of IL-2、 IL-6、IL-8、IL-18 compared with group MA and group NA(P<005 respectively).The serum IL-6、IL-8 levels were positively correlated with ACR level in T2DM patients with DN (r=0.35, P<0.01;r=0.42, P<0.01). Conclusion: IL-6、IL-8 maybe facilitate the development of diabetic nephropathy,and they may be the sensitive indicators to diagnose early stage clinical diabetic nephropathy.
		2013 Vol. 23 (5): 411-413 [Abstract] ( 1171 ) [HTML 1KB] [ PDF 755KB] ( 1440 )

	414	Quantity change and clinical significance of myeloid-derived suppressor cells in peripheral blood of the patients with gastric cancer
		WU Zhi-dan1，2， WANG Sheng-jun1
		Objective: To explore the quantity change and significance of myeloid-derived suppressor cells(MDSCs)in peripheral blood of the patients with gastric cancer.Methods ： Twenty-four patients with gastric cancer were enrolled in this study, collected preoperative peripheral blood 2 mL. And fifteen healthy volunteers were done so too.Selected patients 30 days after operation and restored smoothly 18 cases to be checked,and matched with preoperative data.Collected the peripheral blood and the factors of CD14-/CD11b+/CD33+/HLA-DR- were taken as marks of MDSCs.Analysed the changes of MDSCs number and clinical significance of the proportion using flow cytometry(FCM). At the same time,analyzed the relationship between the expression of MDSCs in peripheral blood and clinical pathological factors.Results： MDSCs in peripheral blood of healthy controls accounted for （0.226±0.044）%,MDSCs proportion of gastric cancer patients with preoperative and postoperative checking in peripheral blood were （1.903±0.247）% and （1.120±0.240）% respectively.The MDSCs proportion difference in gastric cancer pantients and control group was statistically significant(P<0.05),and the MDSCs proportion before and after surgery had significant difference too. MDSCs in the peripheral blood of patients with gastric cancer associated with the degree of tumor differentiation, had nothing to do with gender,age,tumor size, depth of invasion,TNM staging and with or without lymph node metastasis.Conclusions： MDSCs play an important role in gastric cancer patients with immune dysfunction.The bodys antitumor immune function had been some restored after cutting down tumor burden.
		2013 Vol. 23 (5): 414-416 [Abstract] ( 1065 ) [HTML 1KB] [ PDF 749KB] ( 1452 )

	417


		2013 Vol. 23 (5): 417-418,427 [Abstract] ( 1381 ) [HTML 1KB] [ PDF 0KB] ( 533 )

	419	Analysis of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli strains
		YUAN Hong-yan1,2, Guo Bin-fang2, YIN Jing-ping1, SUN Qiang1, GAO Jing-jing1,
		Objective: To analyze aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli（DRECO）strains, and investigate the aminoglycoside resistance mechanisms. Methods: The expressions of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in 20 DRECO strains were assayed by polymerase chain reaction(PCR); and positive PCR products were sequenced by PCR direct automated fluorescence method. Results: In the 20 DRECO strains,18 strains expressed aminoglycoside modifying enzyme genes, the positive rate of aac (3)Ⅱ, aac (6′)Ib, ant (3″)I, aadA4/5 and aph (3′)I were 25%, 25%, 5%, 65% and 55%, respectively, only 1 strains expressed rmtB type 16S rRNA methylase gene, the positive rate was 5%, and the rest genes expressions were not detected. Conclusion: The aadA4/5 and aph (3′)I, not 16S rRNA methylase gene, were closely related with the DRECO aminoglycoside resistance.
		2013 Vol. 23 (5): 419-422,431 [Abstract] ( 1189 ) [HTML 1KB] [ PDF 798KB] ( 1432 )

	423	Association between intraoperative hypotension and outcome after severe traumatic brain injury
		WANG Yun-huan1, XU Min2, CHEN Shu-hua3, LU Xiao-feng2,
		Objective: To analyse association between intraoperative hypotension and outcome after severe traumatic brain injury. Methods: A retrospective analysis was performed of severe TBI patients and recorded next indexes as independent variables: gender, age, diagnosis, preoperative GCS score, pupil, blood pressure, mannitol, intraoperative anesthesia induced hyperventilation, intraoperative hypotension, controlling boost gradient, gradient lower intracranial pressure. Prognostic indicators, including inhospital mortality and days during intensive care unit (ICU), Glasgow outcome score (GOS) at discharge, were recorded as the dependent variables. The differences between each variable was analysed by the binary Logistic regression. Results: There were 41 cases (23.2%) died within one month after surgery, 134 patients survived more than one month. The survival patients′ discharge GOS score 1-3 were 44 cases, and 4-5 90 cases. Intraoperative hypotension was associated with postoperative 1 month mortality and survival ICU stay, but no with hospital discharges GOS. However, with other factors, such as the pupil, the preoperative GCS, intraoperative hypotension did not reach statistical difference, which indicated intraoperative hypotension was not independent relevant factors of postoperative 1 month mortality and ICU stay. Conclusions: Intraoperative hypotension was associated with the mortality in patients with severe traumatic brain injury, but not independent factor and influenced by the severity of the disease. To prevent intraoperative hypotension can improve the prognosis of patients with severe traumatic brain injury.
		2013 Vol. 23 (5): 423-427 [Abstract] ( 1284 ) [HTML 1KB] [ PDF 779KB] ( 1423 )

	428	Effect of dexmedetomidine onshivering after laparoscopic surgery of ectopic pregnancy
		LIU Jian-feng, MA Feng-ya, JIANG Ping
		Objective: To evaluate the effects of dexmedetomidine on shivering after laparoscopic surgery of ectopic pregnancy．Methods: A total of 120 cases ASA I or II patients in the operation under general anesthesia in laparoscopy for ectopic pregnancy were randomly divided into 4 groups(n=30 each)：normal saline control group(group N),dexmedetomidine group(group D) and blood transfusion control group(group Bb),blood transfusion dexmedetomidine group(group Bd).Four groups using the same anesthesia induction and maintenance methods．BIS was maintained at 40-55 during surgery．Group D received dexmedetomidine intravenous infusion at 0.5 μg·(kg·h)-1from immediately after open the vine until 0.5 h before the end of operation， group N received the equal volume of normal saline.Group Bd (when the amount of blood loss greater than 800 mL infusion of blood) received dexmedetomidine intravenous infusion at 0.5 μg·(kg·h)-1 from immediately after opening the vein until 0.5 h before the end of operation and group Bb(when the amount of blood loss greater than 800 mL infusion of blood)received the equal volume of normal saline．The shivering within 1 h after operation was scored and recorded．Results: The incidence rate of postanesthetic shivering was 50％ in group N,57％ in group Bb,20% in group Bd and 10% in group D．Compared with group N，the incidence rate and degree of shivering were decreased in group D(P＜0.05)．Compared with group Bb，the incidence rate and degree of shivering were decreased in group Bd(P＜0.05)．Conclusion: Dexmedetomidineassisted anesthesia can reduce the incidence of postanesthetic shivering in patients with ectopic pregnancy．
		2013 Vol. 23 (5): 428-431 [Abstract] ( 1010 ) [HTML 1KB] [ PDF 770KB] ( 1451 )

	432	Expressions and clinical significance of KPC1 and cytoplasmic p27Kip1 in epithelial ovarian cancer
		WU Ai-min, HAN Yun, CHEN Yan, ZHOU Hong
		Objective: To discuss the expressions and significance of Kip1 ubiquitinationpromoting complex 1（KPC1） and cytoplasmic p27Kip1 in epithelial ovarian cancer. Methods: The expressions of KPC1 and cytoplasmic p27Kip1 in 64 epithelial ovarian cancer samples were detected by immunohistochemical method. The association between their expressions and clinical parameters were analysed. Results: KPC1 and cytoplasmic p27Kip1 expressions were associated with the degree of neoplasm differentiation, FIGO staging and pathological types (both P<0.05). Spearman′s correlation analysis showed KPC1 expression was positively associated with cytoplasmic p27Kip1 (r=0.493,P<0.01). Conclusion: KPC1 and cytoplasmic p27Kip1 might play a synergy role in the oncogenesis and development of ovarian cancer.
		2013 Vol. 23 (5): 432-435 [Abstract] ( 956 ) [HTML 1KB] [ PDF 1274KB] ( 1352 )

	436	Operation approach to children with congenital duodenal diaphragm
		LU Chang-gui, TANG Wei-bing, GEN Qi-ming, XU Xiao-qun, JIANG Wei-wei
		Objective: To determine whether diaphragmectomy or diamond-shaped anastomosis is the more effective treatment in infants with perforate duodenal diaphragm. Methods: Seventy-two infants with congenital duodenal diaphragm were divided into neonatal group(<30 d), younger infant group(30-60 d) and elder infant group(≥60 d). Each age group were divided into diaphragmectomy group and diamond-shaped anastomosis group according to the operation approach. Duration of operation, functional recovery time of alimentary canal, total enteral nutrition, postoperative hospital stay, body weight in 2 months and 6 months after surgery, postoperative incidence of obstructive jaundice, postoperative recurrence incidence of bilious vomiting were recorded and compared. Results: In neonatal group, patients who underwent diaphragmectomy showed shorter operational time, total enteral nutrition time and hospital stay, compared with diamond-shaped anastomosis patients. In younger infant group, patients who underwent diaphragmectomy had shorter operation time and lower weight in 2 months after surgery,but longer total enteral nutrition time and hospital stay, compared with diamond-shaped anastomosis patients. In elder infant group, compared with diamond-shap anastomosis group, patients in diaphragmectomy group showed longer functional recovery time, total enteral nutrition time and hospital stay, higher postoperative recurrence incidence of bilious vomiting (P＜0.05). Conclusion: To younger infants with perforate duodenal diaphragm, the diaphragmectomy was more suitable, while diamond-shaped anastomosis was better to elder ones.
		2013 Vol. 23 (5): 436-439 [Abstract] ( 1235 ) [HTML 1KB] [ PDF 764KB] ( 1851 )

	440	Microanatomy of M1 bifurcation of middle cerebral artery and clinical significance
		ZHAO Hong-xiang1, WANG Zhong2, WU Yu2, YU Nian-zhu3
		Objective: To research on the surface projection of the bifurcation of M1, and the relationship between the M1 bifurcation and the superficial sylvian veins；on purpose it could offer reference data and space quantification concepts for operations operated trough lateral fissure. Methods: On 30 samples of adult heads fixed with formaldehyde solution, we used red latex to pour into the bilateral internal carotids and vertebral arteries, and undertook pterion approach from the left and right separately. We set a coordinate axe on the samples,and measured the number of the projection of the M1 bifurcation on this coordinate.We observed the relationships between the M1 bifurcation and the superficial sylvian veins under different head positions, and measured the vertical distance between them.Results: The projection of the M1 bifurcation was:x-axis of the left (26.56±3.03)mm;y-axis of the left(46.71±4.24)mm;x-axis of the right(27.03±2.92)mm;y-axis of the right: (45.48±4.20)mm.In a circular range with a center located at x=25.0 mm and y=45.0 mm,and a radius of 10 mm,there were 27 samples of projections of M1 segment(90.00%).When the head was turned by 30° toward the opposite side, the M1 bifurcations all lay in the lateral of the superficial sylvian veins, and when turned by 60°, there were 4 samples that lay in the left and 5 samples in the right of the lateral of the superficial sylvian veins, and the rest all lay below the superficial sylvian veins.The vertical distance between the M1 bifurcation and the superficial sylvian vein was:left(14.60 2.27)mm;right(15.03 2.36)mm.Conclusions: The real bifurcation of the M1 segment always lies on the top of the limen of insula.The relationship between the M1 bifurcation and the superficial sylvian vein is closely related to the head position.
		2013 Vol. 23 (5): 440-442,449 [Abstract] ( 1234 ) [HTML 1KB] [ PDF 1297KB] ( 1472 )

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		2013 Vol. 23 (5): 443-445 [Abstract] ( 1018 ) [HTML 1KB] [ PDF 1137KB] ( 1635 )

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		2013 Vol. 23 (5): 446-447 [Abstract] ( 752 ) [HTML 1KB] [ PDF 734KB] ( 1588 )

	448


		2013 Vol. 23 (5): 448-449 [Abstract] ( 872 ) [HTML 1KB] [ PDF 730KB] ( 1542 )

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		2013 Vol. 23 (5): 450-451,456 [Abstract] ( 907 ) [HTML 1KB] [ PDF 1270KB] ( 1278 )

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		2013 Vol. 23 (5): 460-460 [Abstract] ( 539 ) [HTML 1KB] [ PDF 861KB] ( 1598 )

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