耐药大肠埃希菌氨基糖苷类修饰酶和16S rRNA甲基化酶基因的分析

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Abstract Objective: To analyze aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli（DRECO）strains, and investigate the aminoglycoside resistance mechanisms. Methods: The expressions of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in 20 DRECO strains were assayed by polymerase chain reaction(PCR); and positive PCR products were sequenced by PCR direct automated fluorescence method. Results: In the 20 DRECO strains,18 strains expressed aminoglycoside modifying enzyme genes, the positive rate of aac (3)Ⅱ, aac (6′)Ib, ant (3″)I, aadA4/5 and aph (3′)I were 25%, 25%, 5%, 65% and 55%, respectively, only 1 strains expressed rmtB type 16S rRNA methylase gene, the positive rate was 5%, and the rest genes expressions were not detected. Conclusion: The aadA4/5 and aph (3′)I, not 16S rRNA methylase gene, were closely related with the DRECO aminoglycoside resistance.

Received: 01 April 2013

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2013/V23/I5/419

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