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Inhibition of arsenic trioxide on Hedgehog pathway in acute promyelocytic leukemia cell line |
YANG Dong-guang, CAO Feng-lin, YE Xiang-mei, ZHAO Hui, LI Yang, WANG Hong-ling, ZHOU Jin |
(Central Hematology Laboratory, the First Affiliated Hospital of Harbin Medical University, Harbin Heilongjiang 150525, China) |
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Abstract Objective: Human acute promyelocytic leukemia (APL)cell line NB4 was used as a model to discuss whether arsenic trioxide (ATO) can inhibit the expression of Hedgehog signaling pathway molecules,so that we can provide a theoretical basis for the application of ATO in other cancers with an aberrant activation of Hedgehog signaling pathway. Methods: Treated with ATO of different concentration, the expression of Hh pathway components such as Smo,Ptch,Gli1 and Gli2 were analyzed with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western Blot in NB4 at mRNA and protein level.Results: After ATO treatment, the expression of Smo, Ptch and Gli2 were significantly downregulated in NB4. Conclusion: ATO may inhibit the expression of Hh signaling pathway through the mRNA and protein inhibition of the key Gli2 target transcription factor and ATO may inhibit APL partly through the inhibition of Hedgehog signaling pathway.
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Received: 25 April 2013
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