4-aminopyridine suppresses cell migration and invasion of lung cancer待cells SPCA1 and PC9
HU Zhe1,2, HUANG Yu-jie1,3, GU Hong-li1,3, LI Dong-ming1,3, WU Bin1,3, ZHEN Yan1
(1. Institute of Respiratory Diseases, 2. Department of Anesthesiology， 3. Department of Respiratory Medicine， the Affiliated Hospital of Guangdong Medical University, Zhanjiang Guangdong 524001, China)
Abstract：Objective： To explore the effect and molecular mechanism of 4-aminopyridine(4-AP) on migration and invasion of human lung cancer cells. Methods：SPCA1 and PC9 cells were cocultured with 4-AP at various concentrations (0, 3, 4.7 or 0, 3.125, 6.25 mmol/L) for 48 h, respectively. The ability of cell migration and invasion was detected by Transwell migration and invasion assays. Western blotting was used to examine the expression of cell migration and invasionassociated epithelialmesenchymal transition proteins. Results：Compared with negative controls, 4-AP reduced cell migration and invasion in Transwell migration and invasion assays. Meantime, expression of mesenchymal-related genes including Snail and N-cadherin was decreased concomitant with increased expression of epithelialmarker E-cadherin in cells with 4-AP treatment. Conclusion：4-AP may suppress cell migration and invasion in lung cancer cells SPCA1 and PC9 via modulating the expression of migration and invasionassociated epithelialmesenchymal transition proteins.
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2016, Vol. 26 
