rL-IL29对肺腺癌A549细胞生物学特性的影响及相关机制

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摘要目的: 探讨表达IL-29的重组新城疫病毒LoSota株（recombinant Newcastle disease virus LoSota line expressing IL29，rLIL29）对肺腺癌A549细胞增殖、迁移和侵袭的作用及其机制。方法: 体外培养肺腺癌A549细胞，以rL-IL29感染的A549细胞为rL-IL29组，LoSota株新城疫病毒（NDVL）感染的A549细胞为NDVL组及PBS处理的A549细胞为对照组。蛋白质印迹法检测各组A549细胞中NDV HN、IL-29蛋白的表达；CCK8法、划痕试验及Transwell侵袭实验检测rLIL29对肺腺癌A549细胞增殖、迁移和侵袭能力的影响；蛋白质印迹法检测细胞E-钙黏蛋白、MMP2、波形蛋白及AKT/NFκB通路相关蛋白的表达水平。结果： A549细胞感染rL-IL29后，NDV HN及IL-29蛋白均稳定表达，且明显高于NDVL组及对照组；rL-IL29及NDVL感染A549细胞后能够抑制细胞的增殖、迁移及侵袭，且rL-IL29作用更强；与对照组及NDVL组比较，rL-IL29感染后A549细胞中E-钙黏蛋白表达量明显增加，波形蛋白、MMP2（66 kDa/72 kDa）、pAKT及P65蛋白表达明显减弱（均P＜0.05）。结论： rL-IL29可能通过阻碍AKT/NF-κB信号通路上调E-钙黏蛋白表达和下调波形蛋白、MMP2蛋白表达，从而抑制肺腺癌A549细胞增殖、侵袭及迁移。

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关键词 ：重组新城疫病毒, 白细胞介素-29, 肺腺癌, 迁移, 侵袭

Abstract：Objective: To investigate the effect of recombinant Newcastle disease virus expressing IL29（rLIL29） on the proliferation, migration and invasion of lung adenocarcinoma A549 cells and explore the potential mechanism. Methods: A549 cells were cultured in vitro, then infected by rLIL29, Newcastle disease virus LoSota(NDVL) or treated by PBS. The expression of NDV HN and IL29 protein in cells was detected by Western blotting method.The effects of rL-IL29 on the proliferation, migration and invasion of lung adenocarcinoma A549 cell lines were detected by CCK8, scratch test and Transwell invasion test.The expression level of E-cadherin, MMP2, vimentin and AKT/NF-κB pathway associated proteins were detected by Western blotting. Results: NDV and IL-29 protein were stablely expressed in A549 cells infected by rL-IL29, and significantly higher than those of NDVL and control group. After infected with rL-IL29 and NDVL, the ability of proliferation,migration and invasion of A549 cells was inhibited, and rL-IL29 group is more powerful than the control group and NDVL group.The expression level of E-cadherin in the A549 cells was significantly increased after rL-IL29 infection, and the expression of vimentin,MMP2 (66 kDa/72 kDa), p-AKT and P65 protein was all significantly decreased(all P＜0.05). Conclusion: rL-IL29 inhibited the proliferation,invasion and migration of lung adenocarcinoma A549 cells,and the mechanism may be up-regulation of of E-cadherin protein expression and downregulation of MMP2 and vimentin protein expression by inhibiting the AKT/NF-κB signaling pathway.

收稿日期: 2018-01-12

基金资助:

国家自然科学基金资助项目（81672999）;江苏省自然科学基金资助项目（BK20151333）

通讯作者: 步雪峰(通讯作者)，博士，主任医师，硕士生导师，E-mail:xuefengbu05@163.com

作者简介: 严玉兰(1967—)，女，博士，主任医师，硕士生导师。*为共同第一作者；

引用本文:

杭敏1，严玉兰2,*，邵小美1，张日婷1，步雪峰3. rL-IL29对肺腺癌A549细胞生物学特性的影响及相关机制[J]. 江苏大学学报:医学版, 2018, 28(02): 99-103. HANG Min1, YAN Yu-lan2,△, SHAO Xiao-mei1, ZHANG Ri-ting1, BU Xue-feng3. The effect of rL-IL29 on biological characteristics of lung adenocarcinoma A549 cells and related mechanism. Journal of Jiangsu University(Medicine Edition), 2018, 28(02): 99-103.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2018/V28/I02/99

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