Research on transplantation of brainderived neurotrophic factor transfected bone marrow mesenchymal stem cells in therapy of rats with temporary middle cerebral artery occlusion
HE Lan-lan1, ZHANG Zun-sheng2, LIU Yong-hai2
(1.Department of Emergency, the Affiliated People′s Hospital of Zhenjiang, Zhenjiang Jiangsu 212002; 2.Department of Neurology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou Jiangsu 221006, China)
Abstract：Objective: To detect the expression of brain-derived neurotrophic factor(BDNF)transfected bone marrow mesenchymal stem cells(MSCs) in rat brain, which was subjected to ischemia. Methods： MSCs were transfected with BDNF gene by using lipofect technique and its gene expression was detected by immunocytochemistry. Temporary middle cerebral artery occlusion (MCAO) model was established in adult male SD rats, which were randomly divided into control, PBS transplantation,MSCs transplantation and BDNF-transfected MSCs transplantation groups,with 8 rats in each group. At 3 d after modeling, rats in PBS transplantation, MSCs transplantation and BDNF-transfected MSCs transplantation groups were received with phosphate buffered saline, BrdU(bromodeoxyuridine)-labelled MSCs and BDNFtransfected MSCs injection, respectively, whereas control group with no transplantation. Evaluation of nerve function with neurological injury severity score(NSS) was performed after transplantation. The expression of BDNF, neuron-specific enolase and glial fibrillary acidic protein(GFAP) at 30 d after modeling was determined in all groups. Results： The expression of BDNF in labelled MSCs, at 24 h after transfection, was demonstrated by immunohistochhemistry. There were no significant differences in NSS between all groups at 1 d after transplantation. However, the values of NSS in BDNF-transfected MSCs transplantation group were significantly lower than that in other groups at 7, 14, 21, 30 d after transplantation(P＜0.05). At 30 d after transplantation, the number of positive cells of BDNF in BDNF-transfected MSCs transplantation group was higher than that in MSCs transplantation group, and the number of positive cells of NSE and GFAP in BDNF-tansfected group was higher than that in the other three groups. Conclusion： There was expression of BDNF in cerebral ischemia rat, transplanted with BDNF-transfected MSCs, which reduced brain injury by incerasing the expression of NSE and GFAP secreted by nerve cells and glia cells. Transplantation of BDNF-transfected MSCs might be a novel method for treating cerebral ischemia.
收稿日期: 2012-05-31
引用本文:
贺兰兰， 张尊胜， 刘永海. 转染脑源性神经营养因子的骨髓间充质干细胞移植治疗大鼠脑缺血损伤的实验研究[J]. 江苏大学学报:医学版, 2012, 22(6): 476-482.
HE Lan-lan1, ZHANG Zun-sheng2, LIU Yong-hai2. Research on transplantation of brainderived neurotrophic factor transfected bone marrow mesenchymal stem cells in therapy of rats with temporary middle cerebral artery occlusion. Journal of Jiangsu University(Medicine Edition), 2012, 22(6): 476-482.
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2012, Vol. 22 
