Changes and clinical significance of myeloid-derived suppressor cells in peripheral blood of patients with chronic sinusitis with nasal polyps
WU Huihui 1,2， YANG Jun 3， ZENG Xuanfu 1， WANG Ya 2， WANG Shengjun 1,3， MA Yongming 2
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Otorhinolaryngology Head and Neck Surgery, People′s Hospital Affiliated to Jiangsu University, Zhenjiang Jiangsu 212002; 3. Department of Laboratory Medicine, People′s Hospital Affiliated to Jiangsu University, Zhenjiang Jiangsu 212002, China)
Abstract：Objective： To investigate the changes in the ratio and function of myeloidderived suppressor cells (MDSCs) and its subgroups in patients with chronic sinusitis with nasal polyp (CRSwNP), and explore the influence of MDSCs on the occurrence and development of CRSwNP. Methods： Flow cytometry (FCM) was used to detect the ratio of HLA-DR-CD11b+CD33+ MDSCs, HLA-DR-CD11b+CD33+CD14-CD15+ PMN-MDSCs and HLA-DR-CD11b+CD33+CD14+CD15- M-MDSC, and the expression of the functional molecule arginase-1 (ARG-1) in peripheral blood in the CRSwNP group (31 cases) and control group (36 cases). At the same time, the concentration of IL-6 in plasma was detected by enzyme-linked immunosorbent assay. Results： The proportion of total MDSCs in the peripheral blood of CRSwNP patients was significantly higher than that of the control group (P<0.01). Among them, the proportion of PMN-MDSCs in the CRSwNP group was significantly increased (P<0.05); while the proportion of M-MDSCs decreased (P<0.05). The average fluorescence intensity of ARG-1 in the total MDSCs in the CRSwNP group increased (P<0.05), and the plasma IL-6 concentration also increased (P<0.05). Conclusion： The expression of total MDSCs in CRSwNP patients is increased and PMN-MDSCs are the dominant ones, and the expression of ARG-1 is also increased. It suggests that MDSCs may be involved in the occurrence and development of chronic sinusitis.
［Key words］chronic sinusitis; nasal polyps; myeloid-derived suppressor cells and their subgroups; immunosuppression; arginase-1; interleukin-6
