Effect of miR-155-5p on the apoptosis of acute myeloid leukemia cells and its possible mechanism
#br# DENG Zhi-kui1,2, ZHU Wei1
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Hematology, Huai′an First People′s Hospital, Huai′an Jiangsu 223300, China)
Abstract:Objective: To investigate the effect of miR-155-5p on the apoptosis of acute myeloid leukemia (AML) cells and its possible mechanism. Methods: Bone marrow mononuclear cells of 43 AML patients and 21 iron deficiency anemia patients were collected, and the expression of miR1555p and Bcl2 were detected by quantitative reverse transcriptase PCR (qRT-PCR). After AML cells were transfected with miR-155-5p mimics or inhibitors, CCK8 assay was used to detect the cell activity of AML cells treated with different concentration of cytarabine, and flow cytometry was used to detect the apoptosis rate of AML cells that treated with cytarabine(0.16 μg/mL). The mRNA and protein expression levels of NF-κB and Bcl-2 in AML cells which were transfected with miR1555p inhibitor were detected by qRT-PCR and Western blotting, respectively. Results: The mRNA expression level of miR-155-5p and Bcl-2 in AML patients was significantly higher than those in anemia group. Results of CCK-8 and flow cytometry showed that AML cells transfected with miR-155-5p mimic had higher cell activity and lower apoptosis rate after treatment with cytarabine; In contrast, the cell transfected with miR-155-5p inhibitor had lower activity and higher apoptosis rate(P<0.01). The results of qRTPCR and Western blotting showed that the expression levels of NF-κB and Bcl-2 in miR-155-5p inhibitor group were decreased. Conclusion: miR1555p may inhibit AML cell apoptosis by up-regulating the expression of NF-κB and Bcl2.
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